Large-scale double-staining of rat fetal skeletons using Alizarin Red S and Alcian Blue

Teratology

Volumn 61, Issue 4, 2000, Pages 273-276

  Young, Angela D ^a   Phipps, Donal E ^a   Astroff, A Barry ^a  

^a BAYER CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALCIAN BLUE; ALIZARIN RED S;

ANIMAL TISSUE; ARTICLE; CARTILAGE; FETUS; FETUS DEVELOPMENT; GESTATIONAL AGE; INDUSTRY; LABORATORY; NONHUMAN; OSSIFICATION; PRIORITY JOURNAL; RAT; SKELETON; STAINING; TERATOLOGY; TOXICITY TESTING;

ALCIAN BLUE; ANIMALS; ANTHRAQUINONES; BONE AND BONES; COLORING AGENTS; EMBRYONIC AND FETAL DEVELOPMENT; FEMALE; GESTATIONAL AGE; MALE; OSTEOGENESIS; PREGNANCY; RATS; RATS, SPRAGUE-DAWLEY;

ANIMALIA; RODENTIA;

EID: 0034072785     PISSN: 00403709     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-9926(200004)61:4<273::AID-TERA5>3.0.CO;2-2     Document Type: Article

References (6)

1. Inouye M. 1976. Differential staining of cartilage and bone in fetal mouse skeleton by Alcian Blue and Alizarin Red S. Congenital Anomalies 16:171-173.
2. Kimmel CA, Trammel C. 1981. A rapid procedure for routine double staining of cartilage and bone in fetal and adult animals. Stain Technol 56:271-273.
3. Namiki M, Tanaka E, Iwase T, Ikeda Y. 1992. Practical technique for double-staining of unskinned rat fetuses. Teratology. 46:36B.
4. Webb GN, Byrd RA. 1994. Simultaneous differential staining of cartilage and bone in rodent fetuses: an Alcian Blue and Alizarin Red S procedure without glacial acetic acid. Biotechnol Histochem 69:181-185.
5. Whitaker J, Dix KM. 1979. Double staining techniques for rat foetus skeletons in teratological studies. Lab. Anim 13:309-310.
6. US EPA. 1998. Health effects test guidelines, OPPTS 870.3700. Prenatal developmental toxicity study. EPA 712-C-98-207.