The melibiose carrier of Escherichia coli: Cysteine substitutions for individual residues in helix XI

Journal of Membrane Biology

Volumn 174, Issue 2, 2000, Pages 135-140

  Ding, P Z ^a   Wilson, T H ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Cotransporter; Cysteine mutagenesis; Helix XI; Melibiose carrier

Indexed keywords

AMINO ACID; CYSTEINE; MELIBIOSE;

AMINO ACID SUBSTITUTION; ARTICLE; CELL MEMBRANE TRANSPORT; ESCHERICHIA COLI; NONHUMAN; PROTEIN STRUCTURE; PROTEIN TRANSPORT; SITE DIRECTED MUTAGENESIS;

4-CHLOROMERCURIBENZENESULFONATE; AMINO ACID SUBSTITUTION; BIOLOGICAL TRANSPORT; CELL MEMBRANE; CYSTEINE; ESCHERICHIA COLI; LITHIUM; MELIBIOSE; MEMBRANE TRANSPORT PROTEINS; MUTAGENESIS, SITE-DIRECTED; PHENOTYPE; SODIUM; SYMPORTERS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034071910     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002320001038     Document Type: Article

References (21)

1. 1. Akabas, M.H., Stayrrerm, D.A., Xu, M., Karlin, A. 1992. Acetylcholine receptor channel structure probed in cysteine-substitution mutants. Science 258:307-310
2. 2. Botfield, M.C., Wilson, T.H. 1988. Mutations that simultaneously alter both sugar and catin specificity in the melibiose carrier of Escherichia coli. J. Biol. Chem. 263:12909-12915
3. 3. Botfield, M.C., Wilson, T.H. 1989. Peptide-specific antibody for the melibiose carrier of Escherichia coli localizes the carboxyl terminus to the cytoplasmic face of the membrane. J. Biol. Chem. 264:11649-11652
4. 4. Botfield, M.C., Naguchi, K., Tsuchiya, T., Wilson, T.H. 1992. Membrane topology of the melibiose carrier of Escherichia coli. J. Biol. Chem. 267:1818-1822
5. 5. Flitsch, S.L., Khorana, H.G. 1989. Structural studies on transmembrane proteins. 1. model study using bacteriorhodopsin mutants containing single cysteine residues. Biochemistry 28:7800-7805
6. 6. Francis, G., Brennen, L., Stretton, S., Ferenci, T. 1991. Genetic mapping of starch- and lambda-receptor sites in maltoporin: identification of substitutions causing direct and indirect effects of binding sites by cysteine mutagenesis. Mol. Microbiol. 5:2293-2301
7. 7. Gwizdek, C., Leblanc, G., Bassilana, M. 1997. Proteolytic mapping and substrate protection of the Escherichia coli melibiose permease. Biochemistry 36:8522-8529
8. 8. Lolkema, S., Puttner, I.B., Kaback, H.R. 1988. Site-directed mutagenesis of Pro327 in the lac permease of Escherichia coli. Biochemistry 27:8307-8310
9. 9. Lynch, B.A., Koshland, D.E. Jr. 1991. Disulfide cross-linking studies of the transmembrane regions of the aspartate sensory receptor of Escherichia coli. Proc. Natl. Acad. Sci. USA 88:10402-10406
10. 10. Matsuzaki, S., Weissborn, A.C., Tamai, E., Tsuchiya, T., Wilson, T.H. 1999. Melibiose carrier of Escherichia coli: use of cysteine mutagenesis to identify the amino acids on the hydrophilic face of transmembrane helix 2. Biochim. Biophys. Acta 1420:63-72
11. + sugar symport is catalyzed by a single polypeptide. Biochemistry 34:4412-4426
12. 12. Pourcher, T., Bibi, E., Kaback, H.R., Leblanc, G. 1996. Membrane topology of the melibiose permease of Escherichia coli studies by melB-phoA fusion analysis. Biochemistry 35:4161-4168
13. 13. Rosen, B., Tsuchiya, T. 1979. Preparation of everted membrane vesicles from Escherichia coli for the measurement of calcium transport. Methods Enzymol. 56:233-241
14. 14. Sahin-Toth, M., Dunten, R.N., Gonzalez, A., Kaback, H.R. 1992. Functional interactions between putative intramembrane charged residues in the lactose permease of Escherichia coli. Proc. Natl. Acad. Sci. USA 89:10547-10551
15. 15. Weissborn, A.C., Botfield, M.C., Kuroda, M., Tsuchiya, T., Wilson, T.H. 1997. The construction of a cysteine-less melibiose carrier from E. coli. Biochim. Biophys. Acta 1329:237-244
16. 16. D.M. Wilson, T.H. Wilson. 1987. Cation specificity for sugar substrates of the melibiose carrier of Escherichia coli. Biochim. Biophys. Acta 904:191-200
17. 17. Wilson, D.M., Hama, H., Wilson, T.H. 1995. Gly113→Asp can restore activity to the Asp51→Ser mutant in the melibiose carrier of Escherichia coli. Biochem. Biophys. Res. Commun. 209:242-249
18. 18. Wilson, T.H., Wilson, D.M. 1998. Evidence for a close association between helix IV and helix XI in the melibiose carrier of Escherichia coli. Biochim. Biophys. Acta 1374:77-82
19. 19. Yan, R.T., Maloney, P.C. 1995. Residues in the pathway through a membrane transporter. Proc. Natl. Acad. Sci. USA 92:5973-5976
20. 20. Yazyu, H., Shiota-Niiya, S., Shimamoto, T., Kanazawa, H., Futai, M., Tsuchiya, T. 1984. Nucleotide sequence of the melB gene and characteristics of deduced amino acid sequence of the melibiose carrier in Escherichia coli. J. Biol. Chem. 259:4320-4326
21. 21. Zhuang, J., Prive, G.G., Werner, G.E., Ringler, P., Kaback, H.R., Engel, A. 1999. Two-dimensional crystallization of Escherichia coli lactose permease. J. Struct. Biol. 125:63-75