Cloning, overexpression and mutagenesis of cDNA encoding dihydrolipoamide succinyltransferase component of the porcine 2-oxoglutarate dehydrogenase complex

European Journal of Biochemistry

Volumn 267, Issue 10, 2000, Pages 3005-3016

  Koike, Kichiko ^a   Suematsu, Takashi ^a   Ehara, Masahiko ^b  

^a NAGASAKI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b NAGASAKI UNIVERSITY   (Japan)

Author keywords

CDNA; Cloning; Dihydrolipoamide succinyltransferase; Expression; Mutagenesis

Indexed keywords

DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE; OXOGLUTARATE DEHYDROGENASE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE OVEREXPRESSION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; SWINE;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CLONING, MOLECULAR; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; KETOGLUTARATE DEHYDROGENASE COMPLEX; KINETICS; MICROSCOPY, ELECTRON; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OLIGONUCLEOTIDES; PROTEIN BINDING; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SWINE;

EID: 0034071536     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2000.01320.x     Document Type: Article

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