Changes in the electrophoretic profiles of gill mucus proteases of the eastern oyster Crassostrea virginica in response to infection by the turbellarian Urastoma cyprinae

Journal of Invertebrate Pathology

Volumn 75, Issue 2, 2000, Pages 163-170

  Brun, Nicole T ^a   Ross, Neil W ^b   Boghen, Andrew D ^a  

^a UNIVERSITÉ DE MONCTON   (Canada)

^b NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Crassostrea virginica; Eastern oyster; Gills; Mucus; Proteases; SDS PAGE electrophoresis; Turbellaria; Urastoma cyprinae

Indexed keywords

ANIMALIA; BIVALVIA; CRASSOSTREA VIRGINICA; OSTREIDAE; TURBELLARIA; URASTOMA CYPRINAE;

EID: 0034069073     PISSN: 00222011     EISSN: None     Source Type: Journal    
DOI: 10.1006/jipa.1999.4917     Document Type: Article

References (42)

1. Alexander J. B., Ingram G. A. Noncellular nonspecific defence mechanisms of fish. Annu. Rev. Fish Dis. 1992;249-279.
2. Aranishi F., Mano N., Nakane M., Hirose H. Epidermal response of the Japanese eel to environmental stress. Fish Physiol. Biochem. 19:1998;197-203.
3. Avila E. E., Calderon J. Entamoeba histolytica trophozoites: A surface-associated cysteine protease. J. Exp. Parasitol. 76:1993;232-241.
4. Beith J. G. Leukocyte elastase. Barrett A. J., Rawlings N. D., Woessner J. F. Handbook of Proteolytic Enzymes. 1998;54-60 Academic Press, London.
5. Berasain P., Goñi F., McGonigle S., Dowd A., Dalton J. P., Frangione B., Carmona C. Proteinases secreted by Fasciola hepatica degrade extracellular matrix and basement membrane components. J. Parasitol. 83:1997;1-5.
6. Boghen, A. D, Allard, J, and, Bataller, E. 1993, Rapport Final et Recommandations sur le Programme de Monitoring pour la Côte Est du Nouveau-Brunswick, Centre de recherche en sciences de l'environnement, Université de Moncton, Moncton, Nouveau-Brunswick, 76 pp.
7. Bower S. M., McGladdery S. E., Price I. M. Synopsis of infectious diseases and parasites of commercially exploited shellfish. Annu. Rev. Fish Dis. 4:1994;1-199.
8. Bradford M. M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal. Biochem. 72:1976;248-254.
9. Braun R., Arnesen J. A., Rinne A., Hjelmeland K. Immunohistological localization of trypsin in mucus-secreting cell layers of Atlantic salmon, Salmo salarL. J. Fish. Dis. 13:1990;233-238.
10. Brun N. T., Boghen A. D., Allard J. Attraction of Urastoma cyprinae Graff, 1882 (Turbellaria:Urastomidae) to the Eastern Oyster Crassostrea virginica. Dis. Aquat. Org. 37:1999a;139-144.
11. Brun N. T., Boghen A. D., Allard J. Distribution of the turbellarian Urastoma cyprinae on the gills of the Eastern Oyster Crassostrea virginica. J. Shell Res. 18:1999b;175-179.
12. Burt M. D. B., Drinnan R. E. A microturbellarian found in oysters off the coast of Prince Edward Island. J. Fish. Res. Bd. Can. 25:1968;2521-2522.
13. Canicatti C., Ville P., Pagliara P., Roch P. Hemolysins from the mucus of Spirographis spallanzani (Polychaeta:Sabellidae). Mar. Biol. 114:1992;453-458.
14. Cornick J. W., Stewart J. E. Interaction of the pathogen Gaffkya homari with natural defense mechanisms of Homarus americanus. J. Fish. Res. Bd. Can. 25:1968;695-709.
15. Ellis A. E. Non-specific defense mechanisms in fish and their role in disease processes. Dev. Biol. Stand. 49:1981;337-352.
16. Ellis A. E., Masson N., Munro A. L. S. A comparison of proteases extracted from Caligus elongatus (Nordmann, 1832) and Lepeophtheirus salmonis (Kroyer, 1838). J. Fish. Dis. 13:1990;163-165.
17. Fisher W. S. Occurrence of agglutinins in the pallial cavity mucus of oysters. J. Exp. Mar. Biol. Ecol. 162:1992;1-13.
18. Fisher W. S., DiNuzzo A. R. Agglutination of bacteria and erythrocytes by serum from six species of marine molluscs. J. Invertebr. Pathol. 57:1991;380-394.
19. Fleming L. C., Burt M. D. B., Bacon G. B. On some commensal Turbellaria of the Canadian East Coast. Hydrobiologia. 84:1981;131-137.
20. Fleming L. C. Occurrence of symbiotic turbellarians in the oyster, Crassostrea virginica. Hydrobiologia. 132:1986;311-315.
21. Garreis K. A., La Peyre J. F., Faisal M. The effects of Perkinsus marinus extracellular products and purified proteases on oyster defence parameters in vitro. Fish Shellfish Immunol. 6:1996;581-597.
22. Halfon S., Craik C. S. Trypsin. Barrett A. J., Rawlings N. D., Woessner J. F. Handbook of Proteolytic Enzymes. 1998;12-21 Academic Press, London.
23. Hassel M., Klenk G., Frohme M. Prevention of unwanted proteolysis during extraction of proteins from protease-rich tissue. Anal. Biochem. 242:1996;274-275.
24. Hjelmeland K., Christie M., Raa J. Skin mucus protease from rainbow trout, Salmo gairdneri Richardson, and its biological significance. J. Fish. Biol. 23:1983;13-22.
25. Ingram G. A. Substances involved in the natural resistance of fish to infection - A review. J. Fish. Biol. 16:1980;23-60.
26. Kamata I., Yamada M., Uchikawa R., Matsuda S., Arizono N. Cysteine protease of the nematode Nippostrongylus brasiliensis preferentially evokes an IgE/IgG1 antibody response in rats. Clin. Exp. Immunol. 102:1995;71-77.
27. Knox D. P., Jones D. G. Studies on the presence and release of proteolytic enzymes (proteinases) in gastro-intestinal nematodes of ruminants. Int. J. Parasitol. 20:1990;243-249.
28. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
29. McGladdery S. E., Boghen A. D., Allard J. Urastoma cyprinae (Turbellaria) in Eastern Oyster Crassostrea virginica - Population dynamics and effects. Bull. Aquacul. Assoc. Can. 92:1992;17-19.
30. McGladdery S. E., Drinnan R. E., Stephenson M. F. A manual of parasites, pests and diseases of Canadian Atlantic bivalves. Can. Tech. Rep. Fish. Aquat. Sci. 1993.
31. McKerrow J. H. Parasite proteases. Exp. Parasitol. 68:1987;111-115.
32. McKerrow J. H. Workshop summary: The role of proteases in the pathogenesis and immune response to parasitic disease. Agabian N., Goodman H., Nogueira N. Molecular Strategies of Parasitic Invasion. 1989;553-557 A. R. Liss, New York.
33. Perkins P. S., Haley D., Rosenblatt R. Proteolytic enzymes in the blood-feeding parasitic copepod, Phrixocephalus cincinnatus. J. Parasitol. 83:1997;6-12.
34. Plourde S. Incidence du Turbellarié Urastoma cyprinae chez l'Huître Native, Crassostrea virginica. 1991;Université de MonctonThèse d'Initiation à la Recherche, Moncton.
35. Robledo J. A. F., Càceres-Martinez J., Sluys R., Figueras A. The parasitic turbellarian Urastoma cyprinae (Plathyhelminthes:Urastomidae) from blue mussel Mytilus galloprovincialis in Spain: Occurrence and pathology. Dis. Aquat. Org. 18:1994;203-210.
36. Salvesen G. S. Cathepsin G. Barrett A. J., Rawlings N. D., J. F. Woessner. Handbook of Proteolytic Enzymes. 1998;60-62 Academic Press, London.
37. Sung K. C., Dresden M. H. Cysteinyl proteinases of Shistosoma mansoni eggs: Purification and partial characterization. J. Parasitol. 72:1986;891-900.
38. Swain M., Ross N. W. A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate-polyacrylamide gels. Electrophoresis. 16:1995;948-951.
39. Ward J. E. Biodynamics of suspension-feeding in adult bivalve molluscs: Particle capture, processing, and fate. J. Invertebr. Biol. 115:1996;218-231.
40. Ward J. E., Newell R. I. E., Thompson R. J., MacDonald B. A. In vivo studies of suspension-feeding processes in the Eastern Oyster, Crassostrea virginica (Gmelin). Biol. Bull. 186:1994;221-240.
41. White C. A. Jr., Baig S., Robinson P. Taenia saginata oncosphere excretory/secretory peptidases. J. Parasitol. 82:1996;7-10.
42. Woessner J. F. Jr. Matrix metalloproteinases and their inhibitors in connective tissue remodeling. FASEB J. 5:1991;2145-2154.