Shuttle vectors for genetic manipulations in Ustilago maydis

Canadian Journal of Microbiology

Volumn 46, Issue 4, 2000, Pages 333-338

  Kojic, Milorad ^a   Holloman, William K ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

Carboxin; Expression vectors; G418; Hygromycin; Nourseothricin

Indexed keywords

ANTIBIOTIC G 418; CARBOXIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYGROMYCIN; NOURSEOTHRICIN;

ARTICLE; ENZYME ACTIVITY; EXPRESSION VECTOR; GENETIC ANALYSIS; MOLECULAR GENETICS; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; USTILAGO MAYDIS;

FUNGI; MYXOGASTRIA; USTILAGO MAYDIS;

EID: 0034067410     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/w00-002     Document Type: Article

References (19)

1. Bölker, M., Bohnert, H.U., Braun, K.H., Gorl, J., and Kahmann, R. 1995. Tagging pathogenicity genes in Ustilago maydis by restriction enzyme-mediated integration (REMI). Mol. Gen. Genet. 248: 547-552.
2. Bottin, A., Kamper, J., and Kahmann, R. 1996. Isolation of a carbon source-regulated gene from Ustilago maydis. Mol. Gen. Genet. 253: 342-352.
3. Gold, S.E., Bakkeren, G., Davies, J.E., and Kronstad, J.W. 1994. Three selectable markers for transformation of Ustilago maydis. Gene, 142: 225-230.
4. Huffman, C.S., and Winston, F. 1987. A ten minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene, 57: 267-272.
5. Holden, D.W., Kronstad, J.W., and Leong, S.A. 1989. Mutation in a heat-regulated hsp70 gene of Ustilago maydis. EMBO J. 8: 1927-1934.
6. Keon, J.P.R., White, G.A., and Hargreaves, J.A. 1991. Isolation, characterization and sequence of a gene conferring resistance to the systemic fungicide carboxin from the maize smut pathogen, Ustilago maydis. Curr. Genet. 19: 475-481.
7. Kinal, H., Tao, J., and Bruenn, J.A. 1991. An expression vector for the phytopathogenic fungus Ustilago maydis. Gene, 98: 129-134.
8. Kinal, H., Park, C.M., and Bruenn, J.A. 1993. A family of Ustilago maydis expression vectors: new selectable markers and promoters. Gene, 127: 151-152.
9. Krügel, H., Fiedler, G., Smith, C., and Baumberg, S. 1993. Sequence and transcriptional analysis of nourseothricin acetyltransferase-encoding gene nat-1 from Streptomyces noursei. Gene, 127: 127-131.
10. Langridge, J., Langridge, P., and Bergquist, P.L. 1980. Extraction of nucleic acids from agarose gels. Anal. Biochem. 103: 264-271.
11. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
12. Smith. T.L., and Leong, S.A. 1990. Isolation and characterization of a Ustilago maydis glyceraldehyde-3-phosphate dehydrogenase-encoding gene. Gene, 93: 111-117.
13. Straffon, M.J., Hynes, M.J., and Davis, M.A. 1996. Characterization of the ugatA gene of Ustilago maydis, isolation by homology to the gatA gene of Aspergillus nidulans. Curr. Genet. 29: 360-369.
14. Thelen, M.P., Onel, K., and Holloman, W.K. 1994. The REC1 gene of Ustilago maydis involved in the cellular response to DNA damage encodes an exonuclease. J. Biol. Chem. 269: 747-754.
15. Tsang, T., Copeland, V., and Bowden, G.T. 1991. A set of cassette cloning vectors for rapid and versatile adaptation of restriction fragments. Biotechniques, 10: 330.
16. Tsukuda, T., Carleton, S., Fotheringham, S., and Holloman, W.K. 1988. Isolation and characterization of an autonomously replicating sequence from Ustilago maydis. Mol. Cell. Biol. 8: 3703-3709.
17. Tsukuda, T., Bauchwitz, R., and Holloman, W.K. 1989. Isolation of the REC1 gene controlling recombination in Ustilago maydis. Gene, 85: 335-341.
18. Vieira, J., and Messing, J. 1982. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene, 19: 259-268.
19. Wang, J., Holden, D.W., and Leong, S.A. 1988. Gene transfer system for the phytopathogenic fungus Ustilago maydis. Proc. Natl. Acad. Sci. U.S.A. 85: 865-869.