Influence of aging on the synthesis and morphology of the aggrecans synthesized by differentiated human articular chondrocytes

Osteoarthritis and Cartilage

Volumn 8, Issue 3, 2000, Pages 170-179

  Verbruggen, G ^a   Cornelissen, M ^a   Almqvist, K F ^a   Wang, L ^a   Elewaut, D ^a   Broddelez, C ^a   de Ridder, L ^a   Veys, E M ^a  

^a GHENT UNIVERSITY   (Belgium)

Author keywords

Aggrecans; Aging; Chondrocytes

Indexed keywords

AGGRECAN; HYALURONIC ACID;

ADULT; AGED; AGING; ARTICLE; ARTICULAR CARTILAGE; CARTILAGE CELL; CELL PROLIFERATION; CELL SUBPOPULATION; CONTROLLED STUDY; EXTRACELLULAR MATRIX; FEMALE; HUMAN; HUMAN CELL; MALE; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ASSEMBLY; PROTEIN IMMOBILIZATION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS;

EID: 0034066830     PISSN: 10634584     EISSN: None     Source Type: Journal    
DOI: 10.1053/joca.1999.0287     Document Type: Article

References (37)

1. Maroudas A. Glycosaminoglycan turnover in articular cartilage. Phylos Trans R Soc Lond (Ser B). 271:1975;293-313
2. Maroudas A. Metabolism of cartilagenous tissues: A quantitative approach. Maroudas A, Holborow J. Studies in Joint Disease, vol 1. 1980;59-86 Pitman, London
3. Johnstone B, Bayliss MT. The large proteoglycans of the human intervertebral disc. Changes in their biosynthesis and structure with age, topography, and pathology. Spine. 20:1995;674-684
4. Dudhia J, Davidson CM, Wells TM, Vynios DH, Hardingham TE, Bayliss MT. Age-related changes in the content of the C-terminal region of aggrecan in human articular cartilage. Biochem J. 313:1996;933-940
5. Roughley PJ, White RJ. Age-related changes in the structure of the proteoglycan subunits from human articular cartilage. J Biol Chem. 255:1980;217-224
6. Roughley PJ, White RJ. The use of caesium sulphate density gradient centrifugation to analyse proteoglycans from human articular cartllages of different ages. Biochim Biophys Acta. 759:1983;58-66
7. Theocharis DA, Tsiganos CP. Age-related changes of proteoglycan subunits from sheep nasal cartilage. Int J Biochem. 17:1985;479-484
8. Buckwalter JA, Kuettner KE, Thonar EJ-M. Age-related changes in articular cartilage proteoglycans: electron microscopic studies. J Orthop Res. 3:1985;251-257
9. Thonar EJ, Buckwalter JA, Kuettner KE. Maturation-related differences in the structure and composition of proteoglycans synthesised by chondrocytes from bovine articular cartilage. J Biol Chem. 261:1986;2467-2474
10. Buckwalter JA, Roughley PJ, Rosenberg LC. Age-related changes in cartilage proteoglycans. Microsc Res Tech. 28:1994;quantitative electron microscopic studies-408
11. Buckwalter JA, Rosenberg LC. Electron microscopic studies on cartilage proteoglycans. Electron Microsc Rev. 1:1988;87-112
12. Cornelissen M, Verbruggen G, Malfait AM, Veys EM, Dewulf M, Hellebuyck P. Size distribution of native aggrecan aggregates of human articular chondrocytes in agarose. In Vitro Cell Dev Biol. 29:1993;356-358
13. Cornelissen M, Verbruggen G, Malfait AM, Veys EM, Broddelez C, De Ridder L. The study of representative populations of native aggrecan aggregates synthesised by human chondrocytes in vitro. J Tiss Cult Meth. 15:1993;139-146
14. Green WT Jr. Behaviour of articular chondrocytes in cell culture. Clin Orthop. 75:1971;248-260
15. Kuettner KE, Pauli BU, Gall G, McMemoli VA, Schenk RK. Synthesis of cartilage matrix by mammalian chondrocytes in vitro. I. Isolation, culture characteristics and morphology. J Cell Biol. 93:1982;743-750
16. Benya PD, Shaffer JD. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell. 30:1982;215-224
17. Verbruggen G, Veys EM, Wieme N, Malfait AM, Gijselbrecht L, Nimmegeers J. The synthesis and immobilisation of cartilage-specific proteoglycan by human chondrocytes in different concentrations of agarose. Clin Exp Rheumatol. 8:1990;371-378
18. Verbruggen G, Malfait AM, Veys EM, Gyselbrecht L, Lambert J, Almqvist KF. Influence of interferon-gamma on isolated chondrocytes from human articular cartilage. Dose dependent inhibition of cell proliferation and proteoglycan synthesis. J Rheumatol. 20:1993;1020-1026
19. Lebarca C, Paigen K. A simple, rapid, and sensitive DNA assay procedure. Anal Biochem. 102:1980;344-352
20. Pearson JP, Mason RM. The stability of bovine nasal proteoglycans during isolation and storage. Biochim Biophys Acta. 498:1977;176-188
21. Verbruggen G, Malfait AM, Dewulf M, Broddelez C, Veys EM. Standardization of nutrient media for isolated human articular chondrocytes in gelified agarose suspension culture. Osteoarthritis Cart. 3:1995;249-259
22. Malfait, AM, An in vitro model to study interactions between inflammatory cells and chondrocytes, University of Ghent, Gent, 1994, 63, 6
23. Kleinschmidt AK, Zahn RK. Über Desoxyribonucleinsäure Molekulen in Proteinmischfilmen. Z Naturforsch. 146:1959;770-779
24. Delbruck A, Dressow B, Gurr E, Reale E, Schroder H. In vitro culture of human chondrocytes from adult subjects. Connect Tiss Res. 15:1985;155-172
25. Ng CK, Handley CJ, Preston BN, Robinson HC. The ectracellular processing and catabolism of hyaluronan in cultured adult articular cartilage explants. Arch Biochem Biophys. 298:1992;70-79
26. Hardingham TE, Muir H. Binding of Oligosaccharides of Hyaluronic Acid to Proteoglycans. Biochem J. 135:1973;905-908
27. Hascall VC, Heinegard D. Aggregation of cartilage proteoglycans. II. Oligosaccharide competitors of the proteoglycan-hyaluronic interaction. J Biol Chem. 249:1974;4242-4249
28. Fessler JH, Fessler LI. Electron microscopic visualization of the polysaccharide hyaluronic acid. Proc Natl Acad Science 56:1966;141-147
29. Brandt KD, Muir H. Heterogeneity of protein-polysaccharides of porcine articular cartilage. The sequential extraction of chondroitinsulphate-proteins with iso-osmotic neutral sodium acetate. Biochem J. 121:1971;261-270
30. Brandt KD, Muir H. Heterogeneity of protein-polysaccharides of porcine articular cartilage. The chondroitin-sulphate proteins associated with collagen. Biochem J. 123:1971;747-755
31. Bayliss MT. Proteoglycan structure and synthesis. Changes during development and ageing of human articular cartilage. Verbruggen G, Veys EM. Degenerative joints. 1984;13-19 Excerpta Medica, Amsterdam
32. Tang LH, Buckwalter JA, Rosenberg LC. Effect of link protein concentration on articular cartilage proteoglycan aggregation. J Orthop Res. 14:1996;334-339
33. Mundlos S, Meyer R, Yamada Y, Zabel B. Distribution of cartilage proteoglycan (aggrecan) core protein and link protein gene expression during human skeletal development. Matrix. 11:1991;339-346
34. McDougall S, Fu YH, Lowe GN, Williams A, Polendo R, Benya PD. Surface adhesion-mediated regulation of chondrocyte-specific gene expression in the nontransformed RCJ 3.1C5.18 rat chondrocyte cell line. J Bone Miner Res. 11:1996;1130-1138
35. Curtis AJ, Ng CK, Handley CJ, Robinson HC. Effect of insulin-like growth factor-I on the synthesis and distribution of link protein and hyaluronan in explant cultures of articular cartilage. Biochim Biophys Acta. 1135:1992;309-317
36. Holmes MW, Bayliss MT, Muir H. Hyaluronic acid in human articular cartilage. Age-related changes in content and size. Biochem J. 250:1988;435-441
37. Sandy JD, O'Neill JR, Ratzlaff LC. Acquisition of hyaluronate-binding affinity in vivo by newly synthesised cartilage proteoglycans. Biochem J. 258:1989;875-880