Comparative image analysis of EGF immunoreaction in rat submandibular gland using 3,3'-diaminobenzidine with metal enhancer substrate

Biotechnic and Histochemistry

Volumn 75, Issue 1, 2000, Pages 15-18

  Tajima, Yoshifumi ^a   Kawasaki, Mina ^a   Ohno, Jun ^a   Kusama, Kaoru ^a   Maruyama, Shichiro ^a   Kato, Kohtaro ^a  

^a MEIKAI UNIVERSITY SCHOOL OF DENTISTRY   (Japan)

Author keywords

DAB nickel reagent; EGF immunohistochemistry; Image analysis; Rat; Submandibular gland

Indexed keywords

DIAMINOBENZIDINE; EPIDERMAL GROWTH FACTOR;

ANIMAL TISSUE; ARTICLE; COMPARATIVE STUDY; IMAGE ANALYSIS; IMAGE ENHANCEMENT; IMMUNOHISTOCHEMISTRY; NONHUMAN; PROTEIN ANALYSIS; RAT; STAINING; SUBMANDIBULAR GLAND;

EID: 0034062576     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.3109/10520290009047979     Document Type: Article

References (7)

1. Gresik, E. W. 1994. The granular convoluted tubule (GCT) cell of rodent submandibular glands. Microsc. Res. Tech. 27: 1-24.
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3. Hiramatsu, M., Kashimata, M., Takayama, M. and Minami, N. 1994. Developmental changes in and hormonal modulation of epidermal growth factor concentration in the rat submandibular gland. J. Endocrinol. 140: 357-363.
4. Kato, K., Yokose, S. and Tajima, Y. 1991. Use of silver enhancement technique for immunohistochemical detection of epidermal growth factor in rat submandibular gland. Biotech. & Histochem. 1: 87-88.
5. Mori, M. 1991. Histochemistry of the Salivary Glands. CRC Press, Boca Raton, FL. pp. 201-207.
6. Tajima, Y., Kato, K., Utsumi, N. and Hosoi, K. 1994. Computer-aided image analysis applied to immunogold-silver staining: evaluation of proliferating cell nuclear antigen (PCNA)-reactive sites in paraffin sections. Histochemistry 102: 177-181.
7. Tajima, Y., Kato, K., Maruyama, S. and Hosoi, K. 1996. In vivo modulation of proliferating cell nuclear antigen in growth plate chondrocytes from normal, hypophysectomized, and growth hormone-treated hypophysectomized rats: a comparative immunohistochemical study with image analysis. J. Histochem. Cytochem. 44: 713-720.