Preparation of radiolabelled very-low-density lipoprotein in high yield by extended rat liver perfusion

Biotechnology Letters

Volumn 22, Issue 4, 2000, Pages 301-306

  Bennett, Mark J ^a   Hauton, David ^a   Hole, David G ^a   Evans, Rhys D ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Lipoprotein; Liver perfusion; Radiolabelling; Rat liver; Very low density lipoprotein (VLDL)

Indexed keywords

FATTY ACID; LIPID; TRIACYLGLYCEROL; VERY LOW DENSITY LIPOPROTEIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ISOTOPE LABELING; LIPID OXIDATION; LIVER; LIVER PERFUSION; MALE; NONHUMAN; RAT; SYNTHESIS;

EID: 0034058697     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005678517109     Document Type: Article

References (20)

1. Boren J, Veniant MM, Young SG (1998) Apo B100-containing lipoproteins are secreted by the heart. J. Clin. Invest. 101: 1197-1202.
2. Elovson J, Chatterton JE, Bell GT, Schumaker VN, Reuben MA, Puppione DL, et al. (1988) Plasma very low density lipoproteins contain a single molecule of apolipoprotein B. J. Lipid Res. 29: 1461-1473.
3. Evans RD, Wang X (1997) Measurement of fatty acid oxidation in isolated perfused rat heart. A simple and accurate method. Biotechnol. Tech. 11: 127-132.
4. Fielding BA, Callow J, Owen RM, Samra JS, Matthews DR, Frayn KN (1996) Postprandial lipemia: the origin of an early peak studied by specific dietary fatty acid intake during sequential meals. Am. J. Clin. Nutr. 63: 36-41.
5. Gibbons GF, Burnham FJ (1991) Effect of nutritional state on the utilization of fatty acids for hepatitic triacylglycerol synthesis and secretion as very-low-density lipoprotein. Biochem. J. 275: 87-92.
6. Goldfarb S, Pilot HC (1971) Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase. J. Lipid Res. 12: 512-515.
7. Hamilton RL, Williams MC, Fielding CJ, Havel RJ (1976) Discoidal bilayer structure of nascent high density lipoproteins from perfused rat liver. J. Clin. Invest. 58: 667-680.
8. Hems R, Ross BD, Berry MN, Krebs HA (1966) Gluconeogenesis in the perfused rat liver. Biochem. J. 101: 284-292.
9. Hole DG, Bennett MJ, Evans RD (1998) Effect of endotoxin on very-low-density lipoprotein metabolism in rat heart. Biochem. Soc. Trans. 26: S204.
10. Humphreys SM, Frayn KN (1988) Micro-method for preparing perchloric extracts of blood. Clin. Chem. 34: 1657.
11. Krebs HA, Henseleit K (1932) Hoppe-Seyler's Physiol. Chem. 210: 33-66.
12. Lanza Jacoby S, Wong SH, Tabares A, Baer D, Schneider T (1992) Disturbances in the composition of plasma lipoproteins during gram-negative sepsis in the rat. Biochim. Biophys. Acta 1124: 233-240.
13. Marsh JB (1974) Lipoproteins in a nonrecirculating perfusate of rat liver. J. Lipid Res. 15: 544-550.
14. Mayes PA, Felts JM (1976) Effect of haematocrit value and pO2 on the redox state and metabolism of the perfused liver. Biochem. J. 156: 685-689.
15. Nielsen LB, Veniant M, Boren J, Raabe M, Wong JS, Tam C, et al (1998) Genes for apolipoprotein B and microsomal triglyceride transfer protein are expressed in the heart: evidence that the heart has the capacity to synthesize and secrete lipoproteins. Circulation 98: 13-16.
16. Noel SP, Rubinstein D (1981) Uptake of cholesterol from the very low density lipoprotein or its remnants by the perfused rat liver. Can. J. Biochem. 59: 447-453.
17. Schroeder F, Wilcox HG, Keyes WG, Heimberg M (1982) Effects of thyroid status on the structure of the very low density lipoprotein secreted by the perfused liver. Endocrinology 110: 551-562.
18. Wagener H (1967) Methods for separation and determination of lipids. In: Schettler G, ed. Lipids andLipidoses. Berlin: Springer-Verlag, pp. 190-210.
19. Wilcox HG, Dunn GD, Heimberg M (1975) Effects of several common long chain fatty acids on the properties and lipid composition of the very low density lipoprotein secreted by the perfused rat liver. Biochim. Biophys. Acta 398: 39-54.
20. Zilversmit DB, Shea TM (1989) Quantitation of apoB-48 and apoB-100 by gel scanning or radio-iodination. J. Lipid Res. 30: 1639-1646.