Simultaneous immunofluorescent detection of coentrapped cells in gel beads

Applied and Environmental Microbiology

Volumn 66, Issue 5, 2000, Pages 2216-2219

  Prioult, Guenolee ^a   Lacroix, Christophe ^a   Turcotte, Carl ^a   Fliss, Ismaïl ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DAIRY INDUSTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; FERMENTATION; IMMUNOFLUORESCENCE TEST; LACTIC ACID BACTERIUM; LACTOCOCCUS LACTIS CREMORIS; NONHUMAN;

BIFIDOBACTERIUM; CELLS, IMMOBILIZED; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; GELS; LACTOCOCCUS; LACTOCOCCUS LACTIS; MICROSCOPY, CONFOCAL;

BACTERIA (MICROORGANISMS); LACTOCOCCUS LACTIS SUBSP. CREMORIS; POSIBACTERIA; STREPTOCOCCUS;

EID: 0034058048     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.66.5.2216-2219.2000     Document Type: Article

References (15)

1. Cowan, S. E., E. Gilbert, A. Khlebnikov, and J. D. Keasling. 2000. Dual labeling with green fluorescent proteins for confocal microscopy. Appl. Environ. Microbiol. 66:413-418.
2. Haugland, R. P. 1998. Special offer on Alexa probes, p. 12-13. In R. P. Haugland (ed.), BioProbes 27 - new products and applications, February. Molecular Probes, Eugene, Oreg.
3. Hugenholtz, J., H. Veldkamp, and W. N. Konings. 1987. Detection of specific strains and variants of Streptococcus cremoris in mixed cultures by immunofluorescence. Appl. Environ. Microbiol. 53:149-155.
4. Hunik, J. H., M. P. Van den Hoogen, W. de Boer, M. Smit, and J. Tramper. 1993. Quantitative determination of the spatial distribution of Nitrosomas europaea and Nitrobacter agilis cells immobilized in k-carrageenan gel beads by specific fluorescent-antibody labelling technique. Appl. Environ. Microbiol. 59:1951-1954.
5. 3). J. Immunol. Methods 223:155-163.
6. Lacroix, C., I. Sodini, and G. Corrieu. 1996. Microbiological stability of an immobilized cell bioreactor with mixed lactic acid bacteria during continuous fermentation of milk, p. 600-607. In R. Wijffels (ed.), Immobilized cells: basics and applications. Elsevier Science, London, United Kingdom.
7. Lamboley, L., C. Lacroix, C. P. Champagne, and J. C. Vuillemard. 1997. Continuous mixed strain mesophilic lactic starter production in supplemented whey permeate medium using immobilized cell technology. Biotechnol. Bioeng. 56:502-516.
8. Lamboley, L., C. Lacroix, J. M. Artignan, C. P. Champagne, and J. C. Vuillemard. 1999. Long term mechanical and biological stability of an immobilized cell reactor for continuous mixed-strain mesophilic lactic starter production in whey permeate. Biotechnol. Prog. 15:646-654.
9. Mitsuoka, T. 1990. Bifidobacteria and their role in human health. J. Ind. Microbiol. 6:263-267.
10. Ouellette, V., P. Chevalier, and C. Lacroix. 1994. Continuous fermentation of a supplemented milk with immobilized Bifidobacterium infantis. Biotechnol. Tech. 8:45-50.
11. Prévost, H., C. Diviès, and E. Rousseau. 1985. Continuous yoghurt production with Lactobacillus bulgaricus and Streptococcus thermophilus entrapped in Ca-alginate. Biotechnol. Lett. 7:247-252.
12. Roy, D., I. Mainville, and F. Mondou. 1997. Selective enumeration and survival of Bifidobacteria in fresh cheese. Int. Dairy J. 7:785-793.
13. Scott, K. P., D. K. Mercer, L. A. Glover, and H. J. Flint. 1998. The green fluorescent protein as a visible marker for lactic acid bacteria in complex ecosystems. FEMS Microbiol. Ecol. 26:219-230.
14. Sodini, I., C. Y. Boquien, G. Corrieu, and C. Lacroix. 1997. Microbial dynamics of co- and separately entrapped mixed cultures of mesophilic lactic acid bacteria during the continuous prefermentation of milk. Enzyme Microb. Technol. 20:381-388.
15. Sodini-Gallot, I., G. Corrieu, C. Y. Boquien, E. Latrille, and C. Lacroix. 1995. Process performance of continuous inoculation and acidification of milk with immobilized lactic acid bacteria. J. Dairy Sci. 78:1407-1420.