Molecular analysis of hemolysin-mediated secretion of a human interleukin-6 fusion protein in Salmonella typhimurium

FEMS Immunology and Medical Microbiology

Volumn 27, Issue 4, 2000, Pages 333-340

  Li, Yuanyi ^a   Hess, Claudia ^a   Von Specht, Bernd Ulrich ^a   Hahn, Heinz P ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Fusion protein; Hemolysin; Interleukin 6; Protein secretion; Proteolytic cleavage

Indexed keywords

BACTERIAL ANTIGEN; CYTOKINE; HEMOLYSIN; HYBRID PROTEIN; INTERLEUKIN 6; VIRULENCE FACTOR;

ARTICLE; BACTERIAL VIRULENCE; BACTERIUM CULTURE; IMMUNOBLOTTING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN SYNTHESIS; SALMONELLA; SALMONELLA TYPHIMURIUM;

ANIMALS; BACTERIAL PROTEINS; BLOTTING, WESTERN; CELL FRACTIONATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC VECTORS; HEMOLYSIN PROTEINS; HUMANS; INTERLEUKIN-6; MICE; RECOMBINANT FUSION PROTEINS; SALMONELLA TYPHIMURIUM;

EID: 0034057435     PISSN: 09288244     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0928-8244(99)00211-4     Document Type: Article

References (30)

1. Curtiss III, R. (1990) Attenuated Salmonella strains as live vectors for the expression of foreign antigens. In: New Generation Vaccine (Woodrow, G.C. and Levine, M.M., Eds.), pp. 161-188. Marcel Dekker Press, New York.
2. Cardenas L., Clements J. Oral immunization using live attenuated Salmonella ssp. as carriers of foreign antigens. Clin. Microbiol. Rev. 5:1992;328-342.
3. Catmull J., Wilson M.E., Kirchhoff L.V., Metwali A., Donelson J.E. Induction of specific cell-mediated immunity in mice by oral immunization with Salmonella expressing Onchocerca volvulus glutathione S-transferase. Vaccine. 17:1998;31-39.
4. c expressing urease A and B subunits. Infect. Immun. 66:1998;581-586.
5. Akira S., Taga T., Kishimoto T. Interleukin-6 in biology and medicine. Adv. Immunol. 54:1993;1-78.
6. Ramsay A.J., Husband A.J., Ramshaw I.A., Bao S., Matthaei K.I., Koehler G., Kopf M. The role of interleukin-6 in mucosal IgA antibody responses in vivo. Science. 264:1994;561-563.
7. Hess J., Gentschev I., Miko D., Wenzel M., Label C., Goebel W., Kaufmann S.H.E. Superior efficacy of secreted over somatic antigen display in recombinant Salmonella vaccine induce protection against Listeriosis. Proc. Natl. Acad. Sci. USA. 93:1996;1458-1463.
8. Gentschev I., Glaser I., Goebel W., McKeever D.J., Musoke A., Heussler V.T. Delivery of the p67 sporozoite antigen of Theileria parva by using recombinant Salmonella dublin: secretion of the product enhances specific antibody responses in cattle. Infect. Immun. 66:1998;2060-2064.
9. Dunstan S.J., Ramsay A.J., Strugnell R.A. Studies of immunity and bacterial invasiveness in mice given a recombinant salmonella vector encoding murine interleukin-6. Infect. Immun. 64:1996;2730-2736.
10. Gentschev I., Mollenkopf H.-J., Sokolovic Z., Ludwig A., Tengel C., Gross R., Hess J., Demuth A., Goebel W. Synthesis and secretion of bacterial antigens by attenuated Salmonella via the Escherichia coli hemolysin secretion system. Behring Inst. Mitt. 95:1994;57-66.
11. Gentschev I., Dietrich G., Mollenkopf J., Sokolovic Z., Hess J., Kaufmann S.H.E., Goebel W. The Escherichia coli hemolysin secretion apparatus: a versatile antigen delivery system in attenuated Salmonella. Behring Inst. Mitt. 98:1997;103-113.
12. Gentschev I., Mollenkopf H., Sokolovic Z., Hess J., Kaufmann S.H.E., Goebel W. Development of antigen-delivery systems, based on the Escherichia coli hemolysin secretion pathway. Gene. 179:1996;133-140.
13. Mollenkopf H.-J., Gentschev I., Goebel W. Conversion of bacterial gene products to secretion-competent fusion proteins. Biotechniques. 21:1996;854-860.
14. Jarchau T., Chakraborty T., Garcia F., Goebel W. Selection for transport competence of C-terminal polypeptides derived from Escherichia coli hemolysin: the shortest peptide capable of autonomous HlyB/HlyD-dependent secretion comprises the C-terminal 62 amino acids of HlyA. Mol. Gen. Genet. 245:1994;53-60.
15. Kern I., Ceglowski P. Secretion of streptokinase fusion proteins from Escherichia coli cells through the hemolysin transporter. Gene. 163:1995;53-57.
16. Hahn H.P., Hess C., Gabelsberger J., Domdey H., von Specht B.U. A Salmonella typhimurium strain genetically engineered to secrete effectively a bioactive human interleukin (hIL)-6 via the Escherichia coli hemoysin secretion apparatus. FEMS Immunol. Med. Microbiol. 20:1998;111-119.
17. Hess, C. (1999) Expression und Sekretion von humanem Interleukin-6 in lebenden Vakzine Vektoren. PhD Thesis, Eberhard-Karls-University, Tuebingen.
18. Schoedel F., Kelly S.M., Peterson D.L., Milich D.R., Curtiss R. III. Hybrid hepatitis B virus core-pre-S proteins synthesized in avirulent Salmonella typhimurium and Salmonella typhi for oral vaccination. Infect. Immun. 62:1994;1669-1676.
19. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
20. Aarden L.A., De Groot E.R., Schaap O.L., Lansdorp P.M. Production of hybridoma growth factor by human monocytes. Eur. J. Immunol. 17:1987;1411-1416.
21. Elsinghorst E.A. Measurement of invasion by gentamycin resistance. Methods Enzymol. 236:1994;405-420.
22. Krüttgen A., Rose-John S., Möller C., Wroblowski B., Wollmer A., Müllberg J., Hirano T., Kishimoto T., Heinrich P.C. Structure-function analysis of human interleukin-6. Evidence for the involvement of the carboxy-terminus in function. FEBS Lett. 262:1990;323-326.
23. Krüttgen A., Rose-John S., Dufhues G., Bender S., Lütticken C., Freyer P., Heinrich P.C. The three carboxy-terminal amino acids of human interleukin-6 are essential for its biological activity. FEBS Lett. 273:1990;95-98.
24. Vogel M., Hess J., Then I., Juarez A., Goebel W. Characterization of a sequence (hlyR) which enhances synthesis and secretion of hemolysin in Escherichia coli. Mol. Gen. Genet. 212:1988;76-84.
25. Wagner W., Kuhn M., Goebel W. Active and inactive forms of hemolysin in (HlyA) from Escherichia coli. Biol. Chem. Hoppe Seyler. 369:1988;39-46.
26. Brakenhoff J.P.J., Hart M., Aarden L.A. Analysis of hIL-6 mutants expressed in E. coli. Biological activities are not affected by deletion of amino acids 1-28. J. Immunol. 143:1989;1175-1182.
27. Lipinska B., Fayet O., Zylicz M., Georgopoulos C. The HtrA (DegP) protein, essential for Escherichia coli survival at high temperatures, is an exopeptidase. J. Bacteriol. 172:1990;1791-1797.
28. Johnson K., Charles I., Dougon G., Pickard D., O'Gaora P., Costa G., Ali T., Miller I., Hormaeche C. The role of a stress-response protein in Salmonella typhimurium virulence. Mol. Microbiol. 5:1991;401-407.
29. Grodberg J., Dunn J.J. Comparison of Escherichia coli K-12 outer membrane protease Omp T and Salmonella typhimurium E protein. J. Bacteriol. 171:1989;2903-2905.
30. Silver K.R., Keiler K.C., Sauer R.T. Tsp: a tail-specific protease that selectively degrades proteins with nonpolar C-termini. Proc. Natl. Acad. Sci. USA. 89:1992;295-299.