Direct eye visualization of Cy5 fluorescence for immunocytochemistry and in situ hybridization

Journal of Histochemistry and Cytochemistry

Volumn 48, Issue 3, 2000, Pages 437-444

  Ferri, Gian Luca ^a   Isola, Jorma ^d   Berger, Peter ^b   Giro, Gabriele ^c  

^a UNIVERSITY OF CAGLIARI   (Italy)

^b ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^c CNR   (Italy)

^d University of Cagliari at Monserrato ^*

Author keywords

Cy5; FISH; Fluorescence; Fluorescence filter; Fluorochrome; Immunofluorescence; Immunohistochemistry; Multiple staining

Indexed keywords

CYANINE DYE; FLUOROCHROME;

ARTICLE; CAMERA; FLUORESCENCE IN SITU HYBRIDIZATION; FLUORESCENCE MICROSCOPY; IMAGE ANALYSIS; IMMUNOCYTOCHEMISTRY; OBSERVATION; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

EID: 0034054061     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540004800314     Document Type: Article

References (14)

1. Berger P, Staindl B, Wick G (1988) Antigenic features of human protein hormones elucidated by monoclonal antibodies. In Paul JP, McCruden AB, Schuetz PW, eds. Strathclyde Bioengineering Seminars. The Influence of New Technology on Medical Practice. London, MacMillan Press, 212-219
2. Brelje TC, Wessendorf MW, Sorenson RL (1993) Multicolor laser scanning confocal immunofluorescence microscopy: practical applications and limitations. In Matsumoto B, ed. Methods in Cell Biology. Vol 38. New York, Academic Press, 97-181
3. Bridger J, Lampen S, Lichter P (1998) Fluorescence in situ hybridization to DNA. In Spector DL, Goldman RD, Leinwand LA, eds. Cells, a Laboratory Manual. Vol 3. Subcellular Localization of Genes and their Products. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press, 1-45
4. Cullander C (1994) Imaging in the far-red with electronic light microscopy: requirements and limitations. J Microsc 176:281-286
5. Ferri G-L, Gaudio RM, Castello IF, Berger P, Giro G (1997) Quadruple immunofluorescence: a direct visualization method. J Histochem Cytochem 45:155-158
6. Ferri G-L, Gaudio RM, Cossu M, Rinaldi AM, Polak JM, Berger P, Possenti R (1995a) The VGF protein in rat adenohypophysis: sex differences, changes during the estrous cycle and after gonadectomy. Endocrinology 136:2244-2251
7. Ferri G-L, Gaudio RM, Tirolo C, Anedda A, Corpino R (1995b) Simultaneous dual-fluorochrome visualization for co-localization studies. Cell Vis 2:413-419
8. Haugland RP (1996) Handbook of Fluorescent Probes and Research Chemicals. 6th ed. Eugene, OR, Molecular Probes
9. Lennie P, Pokorny J, Smith VC (1993) Luminance. J Opt Soc Am |A| 10:1283-1293
10. Marcus DA (1988) High-performance optical filters for fluorescence analysis. Cell Motil Cytoskel 10:62-70
11. McLean IW, Nakane PK (1974) Periodate-lysine-paraformaldehyde fixative. A new fixative for immunoelectron microscopy. J Histochem Cytochem 22:1077-1083
12. Mujumdar RB, Ernst LA, Mujumdar SR, Lewis CJ, Waggoner AS (1993) Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters. Bioconjug Chem 4:105-111
13. Reichman J (1994) Handbook of Optical Filters for Fluorescence Microscopy. Brattleboro, VT, Chroma Technology
14. Smith AG (1986) Astronomical hypersensitization techniques applied to photomicrography: Kodak Technical Pan film 2415. J Microsc 144:39-44