A simplified procedure for the purification of human N- acetylglucosaminyltransferase-III from human hepatocellular carcinoma tissues

Biotechnology Letters

Volumn 22, Issue 3, 2000, Pages 201-204

  Song, Eun Young ^a   Kim, Kyoung A ^a   Kim, Yung Dai ^a   Kwon, Dur Han ^a   Lee, Hong Soo ^a   Kim, Cheorl Ho ^b   Chung, Tae Wha ^a   Byun, Si Myung ^c  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Dongguk University   (South Korea)

^c Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Hepatocellular carcinoma; Immunoaffinity column; Monoclonal antibody; N acetylglucosaminyltransferase

Indexed keywords

ENZYME ANTIBODY; ISOENZYME; N ACETYLGLUCOSAMINYLTRANSFERASE; SEPHAROSE;

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOAFFINITY CHROMATOGRAPHY; LIVER CELL CARCINOMA;

EID: 0034051786     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005641115696     Document Type: Article

References (5)

1. Ihara Y, Nishikawa A, Tohma T, Soejima H, Nikawa N, Taniguchi N (1993) cDNA cloning, expression, and chromosomal localization of human N-acetylglucosaminyltransferase III (GnT-III). J. Biochem. 113: 692-698.
2. Ishibashi K, Nishikawa A, Hayashi N, Kasahara A, Sato N, Fujii S, Kamada T, Taniguchi N (1989) N-acetylglucosaminyltransferase III in human serum, and liver and hepatoma tissues: increased activity in liver cirrhosis and hepatoma patients. Clin. Chim. Acta 185: 325-332.
3. Kim YJ, Ko JH, Chung DH, Chung TW, Choe IS, Kim CH (1996) Sequence analysis of the 5′-flanking region of the gene encoding human N-acetylglucosaminyltransferase III. Gene 170: 281-283.
4. Mori S, Aoyagi Y, Yanagi M, Suzuki Y, Asakura H (1998) Serum N-acetylglucosaminyltransferase III activities in hepatocellular carcinoma. J. Gastroenterol. Hepatol. 13: 610-619.
5. Nishikawa A, Ihara Y, Hatakeyama M, Kangawa K, Taniguchi N (1992) Purification, cDNA cloning, and expression of UDP-N-acetylglucosamin: β-D-mannoside β-1,4 N-acetylglucosaminyltransferase-III from rat kidney. J. Biol. Chem. 267: 18199-18204.