Molecular cloning and targeted deletion of PEP2 which encodes a novel aspartic proteinase from Aspergillus fumigatus

International Journal of Medical Microbiology

Volumn 290, Issue 1, 2000, Pages 85-96

  Reichard, Utz ^a   Cole, Garry T ^a   Rüchel, Reinhard ^c   Monod, Michel ^b  

^a MEDICAL COLLEGE OF OHIO   (United States)

^b LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^c CHUV ^*

Author keywords

Aspartic proteinase; Aspergillus fumigatus; Cell wall; Fungus; PEP2; Proteinase; Vacuolar proteinase; Vacuole

Indexed keywords

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ASPARTIC PROTEINASE; ASPERGILLOSIS; ASPERGILLUS NIGER; CELL VACUOLE; CELL WALL; COMPLEMENTARY DNA; DELETION MUTAGENESIS; ENZYME ACTIVITY; FUNGAL GROWTH; GENE PROBE; MOLECULAR CLONING; PEP2 GENE; PH; PROTEINASE A; SACCHAROMYCES CEREVISIAE; SIGNAL PEPTIDE;

ASPERGILLUS FUMIGATUS; PICHIA PASTORIS;

EID: 0034051579     PISSN: 14384221     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1438-4221(00)80111-3     Document Type: Article

References (43)

1. Ammerer, G., Hunter, C. P., Rothman, J. H., Saari, G.C., Valls, L. A., Stevens, T. H.: PEP4 gene of Saccharomyces cerevisiae encodes proteinase A, a vacuolar enzyme required for processing of vacuolar precursors. Mol. Cell Biol. 6, 2490-2499 (1986).
2. Anson, M. L.: The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin. J. Gen. Physiol. 22, 79-91 (1938).
3. Barrett, A. J.: Classification of peptidases. Methods Enzymol. 244, 1-15 (1994).
4. Bodey, G. P., Vartivarian, S.: Aspergillosis. Eur. J. Clin. Microbiol. Infect. Dis. 8, 413-437 (1989).
5. Cove, D. J.: The induction and repression of nitrate reductase in the fungus Aspergillus nidulans. Biochim. Biophys. Acta 113, 51-56 (1966).
6. Denning, D.W.: Invasive aspergillosis. Clin. Infect. Dis. 26, 781-805 (1998).
7. Jarai, G., van den Hombergh, H., Buxton, F. P.: Cloning and characterization of the pepE gene of Aspergillus niger encoding a new aspartic protease and regulation of pepE and pepC. Gene 145, 171-178 (1994).
8. Jaton-Ogay, K., Paris, S., Huerre, M., Quadroni, M., Falchetto, R., Togni, G., Latgé, J. P.,Monod, M.: Cloning and disruption of the gene encoding an extracellular metalloprotease of Aspergillus fumigatus. Mol. Microhiol. 14, 917-928 (1994).
9. Jaton-Ogay, K., Suter, M., Crameri, R., Falchetto, R., Fatih, A., Monod, M.: Nucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline protease of Aspergillus fumigatus. FEMS Microbiol. Lett. 71, 163-168 (1992).
10. Jones, F.W., Webb, G.C., Hiller, M.A.: Biogenesis and Function of the Yeast Vacuole. In: The Molecular and Cellular Biology of the Yeast Saccharomyces: Cell Cycle and Cell Biology (J. R. Pringle, J. R. Broach and E. W. Jones, eds.), vol. 3, pp. 363-470. Cold Spring Harbor Laboratory Press, New York 1997.
11. Klionsky, D.J., Herman, P. K., Emr, S.D.: The fungal vacuole: composition, function, and biogenesis. Microbiol. Rev. 54, 266-292 (1990).
12. Kwon-Chung, K. J., Bennett, J.: Aspergillosis. In: Medical Mycology, pp. 201-247. Lea & Febiger, Philadelphia 1992.
13. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685 (1970).
14. Latgé, J. P.: Aspergillus fumigatus and aspergillosis. Clin. Microbiol. Rev. 12, 310-350 (1999).
15. Lee, J. D., Kolattukudy, P. E.: Molecular cloning of the cDNA and gene for an elastinolytic aspartic proteinase from Aspergillus fumigatus and evidence of its secretion by the fungus during invasion of the host lung. Infect. Immun. 63, 3796-3803 (1995).
16. Lott, T. J., Page, L. S., Boiron, P., Benson, J., Reiss, E.: Nucleotide sequence of the Candida albicans aspartyl proteinase gene. Nucleic Acids Res. 17, 1779 (1989).
17. Marshall, R. D.: Glycoproteins. Annu. Rev. Biochem. 41, 673-702 (1972).
18. Money, N. P., Hill, T. W.: Correlation between endoglucanase secretion and cell wall strength in oomycete hyphae: implications for growth and morphogenesis. Mycologia 89, 777-785 (1997).
19. Monod, M., Paris, S., Sanglard, D., Jaton-Ogay, K., Bille, J., Latgé, J. P.: Isolation and characterization of a secreted metalloprotease of Aspergillus fumigatus. Infect. Immun. 61, 4099-4104 (1993).
20. Monod, M., Togni, G., Rahalison, L., Frenk, E.: Isolation and characterisation of an extracellular alkaline protease of Aspergillus fumigatus. J. Med. Microbiol. 35, 23-28 (1991).
21. Moudni, F. B., Rodier, M. H., Babin, P., Jacquemin, J. L.: Metallopeptidase in Candida albicans: immunolocalization in yeast. J. Mycol. Med. 7, 5-9 (1997).
22. Paris, S.: Isolation of protease negative mutants of Aspergillus fumigatus by insertion of a disrupted gene. In: Molecular Biology of Pathogenic fungi. A Laboratory Manual (B. Maresca and G. S. Kobayashi, eds), pp. 49-55. Telos Press, New York 1994.
23. Punt, S. J., Oliver, P. O., Dingemanse, M. A., Pouwels, P. H., van den Hondel, C. A. M. J. J.: Transformation of Aspergillus based on hygromycin B resistance marker from Escherichia coli. Gene 56, 117-124 (1987).
24. Rawlings, N. D., Barrett, A. J.: Families of aspartic peptidases, and those of unknown catalytic mechanism. Methods Enzymol. 248, 105-120 (1995).
25. Reichard, U., Büttner, S., Eiffert, H., Staib, F., Rüchel, R.: Purification and characterisation of an extracellular serine proteinase from Aspergillus fumigatus and its detection in tissue. J. Med. Microbiol. 33, 243-251 (1990).
26. Reichard, U., Eiffert, H., Rüchel, R.: Purification and characterization of an extracellular aspartic proteinase from Aspergillus fumigatus. J. Med. Vet. Mycol. 32, 427-436 (1994).
27. Reichard, U., Monod, M., Odds, F., Rüchel, R.: Virulence of an aspergillopepsin-deficient mutant of Aspergillus fumigatus and evidence for another aspartic proteinase linked to the fungal cell wall. J. Med. Vet. Mycol. 35, 189-196 (1997).
28. Reichard, U., Monod, M., Rüchel, R.: Molecular cloning and sequencing of the gene encoding an extracellular aspartic proteinase from Aspergillus fumigatus. FEMS Microbiol. Lett. 130, 69-74 (1995).
29. Reichard, U., Monod, M., Rüchel, R.: Expression pattern of aspartic proteinase antigens in aspergilli. Mycoses 39, 99-101 (1996).
30. Rothman, J. H., Hunter, C. P., Valls, L. A., Stevens, T. H.: Overproduction-induced mislocalization of a yeast vacuolar protein allows isolation of its structural gene. Proc. Natl. Acad. Sci. USA 83, 3248-3252 (1986).
31. Rüchel, R.: Properties of a purified proteinase from the yeast Candida albicans. Biochim. Biophys. Acta 659, 99-113 (1981).
32. Rüchel, R., Reichard, U.: Pathogenesis and clinical presentation of aspergillosis. In: Aspergillus fumigatus. Biology, Clinical Aspects and Molecular Approaches to Pathogenicity (A. Brakhage, B. Jahn and A. Schmidt, eds), vol. 2, pp. 21-43. Karger AG, Basel 1999.
33. Sambrook, J., Fritsch, E. F., Maniatis, T.: Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, New York 1989.
34. Scharmann, W., Balke, E.: [Studies on a protease (elastase) from Pseudomonas aeruginosa, I: formation and purification of the protease (author's transl)]. Hoppe Seylers Z. Physiol. Chem. 355, 443-450 (1974).
35. Tang, C. M., Cohen, J., Krausz, T., Van Noorden, S., Holden, D.W.: The alkaline protease of Aspergillus fumigatus is not a virulence determinant in two murine models of invasive pulmonary aspergillosis. Infect. Immun. 61, 1650-1656 (1993).
36. Tilburn, J., Scazzocchio, C., Taylor, G. G., Zabicky-Zissman, J. H., Lockington, R. A., Davies, R. W.: Transformation by integration in Aspergillus nidulans. Gene 26, 205-221 (1983).
37. van den Hombergh, J. P., Sollewijn Gelpke, M. D., van de Vondervoort, P. J., Buxton, F. P., Visser, J.: Disruption of three acid proteases in Aspergillus niger - effects on protease spectrum, intracellular proteolysis, and degradation of target proteins. Eur. J. Biochem. 247, 605-613 (1997).
38. Vazquez-Laslop, N., Tenney, K., Bowman, B. J.: Characterization of a vacuolar protease in Neurospora crassa and the use of gene RIPing to generate protease-deficient strains. J. Biol. Chem. 271, 21 944-21 949 (1996).
39. von Heijne, G.: A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 14, 4683-4690 (1986).
40. Warburg, O., Christian, W.: Isolierung und Kristallisation des Gärungsfermentes Enolase. Biochemische Zeitschrift 310, 384-421 (1942).
41. Woolford, C. A., Daniels, L.B., Park, F. J., Jones, E. W., Van Arsdell, J. N., Innis, M. A.: The PEP4 gene encodes an aspartyl protease implicated in the posttranslational regulation of Saccharomyces cerevisiae vacuolar hydrolases. Mol. Cell. Biol. 6, 2500-2510 (1986).
42. Yuan, L., Cole, G. T.: Isolation and characterization of an extracellular proteinase of Coccidioides immitis. Infect. Immun. 55, 1970-1978 (1987).
43. Zubenko, G. S., Jones, E. W.: Protein degradation, meiosis and sporulation in proteinase-deficient mutants of Saccharomyces cerevisiae. Genetics 97, 45-64 (1981).