Transport and consumption rate of O2 in alginate gel beads entrapping hepatocytes

Biotechnology Letters

Volumn 22, Issue 10, 2000, Pages 865-870

  Annesini, M Cristina ^a   Castelli, Giuseppe ^a   Conti, Filippo ^a   Conti De Virgiliis, Laura ^b   Marrelli, Luigi ^a   Miccheli, Alfredo ^a   Satori, Elisabetta ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b UNIVERSITY OF L'AQUILA   (Italy)

Author keywords

Alginate; Bioartificial liver; Hepatocytes

Indexed keywords

ALGINIC ACID;

ANIMAL CELL; ARTICLE; ARTIFICIAL LIVER; BIOREACTOR; CELL DENSITY; IMMOBILIZED CELL; LIVER CELL; MALE; NONHUMAN; OXYGEN CONSUMPTION; OXYGEN TRANSPORT; OXYGENATION; RAT;

EID: 0034046682     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005623732252     Document Type: Article

References (19)

1. 2 supply during prolonged isolated rat liver perfusion. J. Pharmacol. Toxicol. Meth. 39: 185-192.
2. Foy BD, Lee J, Morgan J, Toner M, Tompkins RJ, Yarmush ML (1993) Optimization of hepatocyte attachment to microcarriers: importance of oxygen. Biotechnol. Bioeng. 42: 579-588.
3. Jauregui HO (1997) The technology of biological extracorporeal liver assist devices: from infancy to adolescence. Artif. Organs 21: 1163-1168.
4. Jen AC, Wake MC, Mikos AG (1996) Review: hydrogels for cell immobilization. Biotechnol. Bioeng. 50: 357-364.
5. Kamlot A, Rozga J, Watanabe FD, Demetriou AA (1996) Review: artificial liver support systems. Biotechnol. Bioeng. 50: 382-391.
6. 2 limitation. Biotechnol. Lett. 20: 549-552
7. King G, Goosen MFA (1993) Cell immobilization technology: an overview. In: Goosen MFA, ed. Fundamentals of Animal Cell Encapsulation and Immobilization. Boca Raton: CRC Press, pp. 1-6
8. Kurosawa H, Matsumura M, Tanaka H (1989) Oxygen diffusivity in gel beads containing viable cells. Biotechnol. Bioeng. 34: 926-932.
9. Martinsen A, Skjak-Braek G, Smidsrod O (1989) Alginate as immobilization material: I. Correlation between chemical and physical properties of alginate gel beads. Biotechnol. Bioeng. 33: 79-89.
10. Miccheli A, Capuani G, Tomassini A, Di Cocco ME, Manetti C, Conti De Virgiliis L, Falasca L, Manganaro M, Conti F (1998) NMR evaluation of viability and metabolism of hepatocytes entrapped at high cell density in alginate gel beads in a bioreactor. Int. J. Artif. Organs 21: 656.
11. Moldeus P, Hogberg J, Orrenius S (1978) Isolation and use of liver cells. Meth. Enzymol. 52: 60-68.
12. Nishikawa M, Uchino J, Matsushita M, Takahashi M, Taguchi K, Koike M, Kamachi H, Kon H (1996) Optimal oxygen tension conditions for functioning cultured hepatocytes in vitro. Artif. Organs 20: 169-177.
13. Ohno K, Maier P (1994) Cultured rat hepatocytes adapt their cellular glycolytic activity and adenilate energy status to tissue oxygen tension: influences of extracellular matrix component, insulin and glucagon. J. Cell Physiol. 160: 358-366.
14. Riordan S, Williams SR (1997) Bioartificial liver support: developments in hepatocyte culture and bioreactor design. Br. Med. Bull. 53: 730-744.
15. Rotem A, Toner M, Bhatia S, Foy BD, Tompkins RG, Yarmush ML (1994). Optimal oxygen tension conditions for functioning cultured hepatocytes in vitro. Biotechnol. Bioeng. 43: 654-660.
16. Selden C, Roberts E, Stamp G, Parker K, Winlove P, Ryder T, Platt H, Hodgson H (1998) Comparison of three solid phase support for promoting three-dimensional growth and function of human liver cell lines. Artif. Organs 22: 308-319.
17. Shoichet MS, Li RH, White ML, Winn SR (1996) Stability of hydrogels used in cell encapsulation: an in vitro comparison of alginate and agarose. Biotechnol. Bioeng. 50: 374-381.
18. Tanaka H, Matsumura M, Veliky IA (1984) Diffusion characteristics of substrates in Ca-alginate gel beads. Biotechnol. Bioeng. 26: 53-58.
19. Wolfe D, Jungermann K (1985) Long-term effects of physiological ozygen concentration on glycolysis and gluconeogenesis in hepatocyte cultures. Eur. J. Biochem. 151: 299-303.