DNA and buffers: The hidden danger of complex formation

Biopolymers

Volumn 54, Issue 2, 2000, Pages 137-142

  Stellwagen, Nancy C ^a   Gelfi, Cecilia ^b   Righetti, Pier Giorgio ^c  

^a UNIVERSITY OF IOWA   (United States)

^b CNR   (Italy)

^c UNIVERSITY OF VERONA   (Italy)

Author keywords

Agarose gels; DNA; DNA capillary electrophoresis; DNA gel electrophoresis; DNA borate complexes; Electroendosmotic flow

Indexed keywords

BORON COMPOUNDS; COMPLEXATION; ELECTROCHEMISTRY; ELECTROPHORESIS; GELS; IONS; POLYACRYLATES; SODIUM; SUGARS;

ELECTROENDOSMOTIC FLOW;

DNA;

BORIC ACID; BUFFER; DNA;

AGAR GEL ELECTROPHORESIS; ARTICLE; CAPILLARY ELECTROPHORESIS; COMPLEX FORMATION; DNA BINDING; DNA HELIX; ELECTROCHEMISTRY; MOLECULAR DYNAMICS; MOLECULAR INTERACTION;

ACETATES; BORATES; BORIC ACIDS; BUFFERS; DNA; EDETIC ACID; ELECTROPHORESIS; ETHYLENEDIAMINES; GELS; TROMETHAMINE;

EID: 0034045438     PISSN: 00063525     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0282(200008)54:2<137::AID-BIP6>3.0.CO;2-I     Document Type: Article

References (29)

1. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular Cloning: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Plainview NY, 1987.
2. Stellwagen, N. C.: Gelfi, C.; Righetti, P. G. Biopolymers 1997, 42, 687-703.
3. Olivera, B. M.: Baine, P.; Davidson, N. Biopolymers 1964, 2, 245-257.
4. Manning, G. Q. Rev Biophys 1978, 11, 179-246.
5. Overbeek, J. T. G.; Wiersema, P. H. In Electrophoresis: Bier, M., Ed.; Academic: New York, 1967; pp 1-52.
6. Strutz, K.; Stellwagen, N. C. Electrophoresis 1998, 19, 635-642.
7. Holmes, D. L.; Stellwagen, N. C. Electrophoresis 1991, 12, 254-263.
8. Holmes, D. L.; Stellwagen, N. C. Electrophoresis 1990, 11, 5-11.
9. West, R. Biopolymers 1987, 26, 607-608.
10. . Simó-Alfonso, E.; Gelfi, C.; Sebastiano, R.; Citterio, A.; Righetti, P. G. Electrophoresis 1996, 17, 732-737.
11. Stellwagen, N. C. Electrophoresis 1992, 13, 601-603.
12. Mohanty, U.; Stellwagen, N. C. Biopolymers 1999, 49, 209-214.
13. Costantino, L.; Liquori, A. M.; Vitagliano, V. Biopolymers 1964, 2, 1-8.
14. Ross, P. D.; Scruggs, R. L. Biopolymers 1964, 2, 231-236.
15. Anderson, C. F.; Record, M. T., Jr.; Hart, P. A. Biophys Chem 1978, 7, 301-316.
16. Wenner, J. R.; Bloomfield, V. A. Anal Biochem 1999, 268, 201-212.
17. Muetterties, E. L. The Chemistry of Boron and Its Compounds; John Wiley & Sons: New York, 1967.
18. Ferguson, K. A. Metabolism 1964, 13, 985-1002.
19. Morris, C. J. O. R.; Morris, P. Biochem J 1971, 124, 517-528.
20. Rodbard, D.; Chrambach, A. Proc Natl Acad Sci USA 1970, 65, 970-977.
21. Rees, D. A.; Morris, E. R.; Thom, D.; Madden, J. K. In The Polysaccharides, Aspinall, G. O., Ed.; Academic: New York, 1982; Vol 1, pp 238-245.
22. Foster, A. B. In Advances in Carbohydrate Chemistry; Wolfrom, M. L., Ed.; Academic: New York, 1957; Vol 12, pp 81-115.
23. Klockow, A.; Amado, R.; Widmer, H. M.; Paulus, A. Electrophoresis 1996, 17, 110-119.
24. Denuziere, A.: Taverna, M.; Ferrier, D.; Domard, A. Electrophoresis 1997, 18, 745-750.
25. Plocek, J.; Chmelik, J. Electrophoresis 1997, 18, 1148-1152.
26. The Agarose Monograph, 4th ed.; FMC Corporation: Rockland, ME, 1988.
27. Serwer, P. Electrophoresis 1983, 4, 375-382.
28. Gelfi, C.; Mauri, E.; Perduca, M.; Stellwagen, N. C.; Righetti, P. G. Electrophoresis 1998, 19, 1704-1710.
29. Stellwagen, N. C.; Gelfi, C.; Righetti, P. G. J Chromatogr A 1999, 838, 179-189.