Cloning and secreted expression of the extracellular domain of the mumps virus fusion protein in Pichia pastoris

Virus Genes

Volumn 20, Issue 2, 2000, Pages 127-133

  McAleer, Barry ^a,b   Rima, Bertus ^a  

^a Medical Biology Centre   (United Kingdom)

^b ANIMAL HEALTH TRUST   (United Kingdom)

Author keywords

AOX1; Metabolic labeling; One step transformation; Secreted expression; Signal peptide; Western blotting

Indexed keywords

HYBRID PROTEIN; PROTEINASE; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; VIRUS PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; MOLECULAR CLONING; MUMPS VIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SECRETION; SACCHAROMYCES CEREVISIAE; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CERCOPITHECUS AETHIOPS; CLONING, MOLECULAR; METHANOL; MOLECULAR SEQUENCE DATA; MUMPS VIRUS; PICHIA; PRECIPITIN TESTS; PROTEIN STRUCTURE, TERTIARY; TEMPERATURE; TRANSFORMATION, GENETIC; VERO CELLS; VIRAL FUSION PROTEINS;

MUMPS VIRUS; PICHIA PASTORIS; RNA VIRUSES; SACCHAROMYCES CEREVISIAE;

EID: 0034045105     PISSN: 09208569     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008162329499     Document Type: Article

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