Fluorolabeling of antibody variable domains with green fluorescent protein variants: Application to an energy transfer-based homogeneous immunoassay

Protein Engineering

Volumn 13, Issue 5, 2000, Pages 369-376

  Arai, Ryoichi ^a   Ueda, Hiroshi ^a,b   Tsumoto, Kouhei ^c   Mahoney, Walt C ^d   Kumagai, Izumi ^c   Nagamune, Teruyuki ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b MEDICAL RESEARCH COUNCIL   (United Kingdom)

^c TOHOKU UNIVERSITY   (Japan)

^d Roche Diagnostics   (United States)

Author keywords

Antibody variable region; Chimeric protein; Fluorescence resonance energy transfer; Fluoroimmunoassay; Green fluorescent protein

Indexed keywords

ANTIBODY; CHIMERIC PROTEIN; GREEN FLUORESCENT PROTEIN; LYSOZYME; THIOREDOXIN;

AFFINITY CHROMATOGRAPHY; ANTIGEN BINDING; ARTICLE; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; FLUORESCENCE ANALYSIS; IMMUNOASSAY; IMMUNOGLOBULIN VARIABLE REGION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION;

EID: 0034043555     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/13.5.369     Document Type: Article

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