Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates

World Journal of Gastroenterology

Volumn 6, Issue 3, 2000, Pages 365-370

  Gao, Yi ^a   Hu, Huan Zhang ^a   Chen, Ke ^a   Yang, Ji Zhen ^a  

^a SOUTHERN MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMATERIAL;

ARTICLE; ARTIFICIAL LIVER; CELL CULTURE; LIVER CELL; NONHUMAN; PORTAL VEIN BLOOD; SWINE;

EID: 0034039550     PISSN: 10079327     EISSN: None     Source Type: Journal    
DOI: 10.3748/wjg.v6.i3.365     Document Type: Article

References (13)

1. Chen K, Gao Y, Pan YX, Yang JZ. An effective system for culturing primary porcine hepatocytes. Shijie Huaren Xiaohua Zazhi , 1999;7:206-209
2. Kimura M, Ogihara M. Proliferation of adult rat hepatocytes in primary culture induced by insulin is potentiated by cAMP-elevating agents. Eur J Pharmacol , 1997;327:87-95
3. Kono Y, Yang S, Roberts EA. Extended primary culture of human hepatocytes in a collagen gel sandwich system. In Vitro Cell Dev Biol Anim , 1997;33:467-472
4. Taguchi K, Matsushita M, Takahashi M, Uchino J. Development of a bioartificial liver with sandwiched-cultured hepatocytes between two collagen gel layers. Artif Organs , 1996;20:178-185
5. Koike M, Matsushita M, Taguchi K, Uchino J. Function of culturing monolayer hepatocytes by collagen gel coating and co-culture with nonparenchymal cells. Artif Organs , 1996;20:186-192
6. Schuppan D, Schmid M, Somasundaram R, Ackermann R, Ruehl M, Nakamura T, Riecken EO. Collagens in the liver extracellular matrix bind hepatocyte growth factor. Gastroenterology , 1998;114:139-152
7. Hu MY, Cipolle M, Sielaff T, Lovdahl MJ, Mann HJ, Remmel RP, Cerra FB. Effect of hepatocytes growth factor on viability and biotransformation function of hepatocytes in gel entrapped and monolayer culture. Crit Care Med , 1995;23:1237-1242
8. Flendrig LM, Te Velde AA, Chamuleau RA. Semipermeable hollow fiber membranes in hepatocyte bioreactors a prerequisite for a successful bioartificial liver? Artif Organs , 1997;21:1177-1181
9. Sielaff TD, Myberg SL, Rollins MD, Hu MY, Amiot B, Lee A, Wu FJ, Hu WS, Cerra FB. Characterization of the three-compartment gelentrapment porcine hepatocytes bioartificial liver. Cell Biol Toxicol , 1997;13:357-364
10. Landry J, Bernier D, Quellet C, Goyette R, Marceau N. Spheroidal aggregate culture of rat liver cells: histotypic reorganization, biomatrix deposition and maintenance of functional activities. J Cell Biol , 1985; 101:914-923
11. Clayton DF, Harrelson AL, Darnell JE Jr. Dependence of liver specific transcription on tissue organization. Mol Cell Biol , 1985;5:2623-2632
12. Sawamoto K, Takahashi N. Modulation of hepatocyte function by changing the cell shape in primary culture. In Vitro Cell Dev Biol Anim , 1997;33:569-574
13. Sakai Y, Naruse K, Nagashima I, Muto T, Suzuki M. Large-scale preparation and function of procine hepatocytes spheroids, Int J Artif Organs , 1996;19:294-301