Quantitative assessment of marine sponge cells in vitro: Development of improved growth medium

In Vitro Cellular and Developmental Biology - Animal

Volumn 36, Issue 3, 2000, Pages 194-200

  Willoughby, Robin ^a,b   Pomponi, Shirley A ^a  

^a FLORIDA ATLANTIC UNIVERSITY   (United States)

^b Div Biomed Mar Res Harbor Br O   (United States)

Author keywords

Cell culture; Fluorescein diacetate; Hoechst; Invertebrate; Porifera; Sponge; Sulforhodamine B

Indexed keywords

DNA; ESTERASE; FLUORESCEIN DIACETATE; GLUTAMINE; M PROTEIN; SELENIUM; SULFORHODAMINE B;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DENSITY; CELL DIVISION; CELL GROWTH; CELL VIABILITY; CULTURE MEDIUM; DNA CONTENT; ENZYME ACTIVITY; IN VITRO STUDY; NONHUMAN; PROTEIN CONTENT; SPONGE (PORIFERA);

ANIMALS; CULTURE MEDIA; ESTERASES; GLUTAMINE; PORIFERA; SELENIUM;

ANIMALIA; INVERTEBRATA; MORCHELLA; PORIFERA; TEICHAXINELLA MORCHELLA;

EID: 0034034859     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1290/1071-2690(2000)036<0194:QAOMSC>2.3.CO;2     Document Type: Article

References (22)

1. Albitzi, K. F.; Faulkner, D. J. Stevensine, a novel alkaloid of an unidentified marine sponge. J. Org. Chem. 50:4163-4164; 1985.
2. Dorsey, J.; Yentsch, C. M.; Mayo, S.; McKenna, C. Rapid analytical technique for the assessment of cell metabolic activity in marine microalgae. Cytometry 10:622-628; 1989.
3. Freshney, R. I. Culture of animal cells. 3rd ed. New York, NY: Wiley-Liss; 1994.
4. Giano, E.; Magnino, G.; Burlando, B.; Sara, M. Morphological responses of dissociated sponge cells to different organic substrata. Tissue Cell 25: 333-341; 1993.
5. Gramzow, M.; Schroder, H. C.; Fritsche, U., et al. Role of phospholipase A2 in the stimulation of sponge cell proliferation by homologous lectin. Cell 59:939-948; 1989.
6. Graph Pad Software. InStat guide to choosing and interpreting statistical tests. San Diego, CA: GraphPad Software; 1989.
7. Ham, R. G.; McKeehan, W. L. Media and growth requirements. Methods Enzymol. 58:44-93; 1979.
8. Ilan, M.; Contini, H.; Carmeli, S.; Rinkevich, B. Progress towards cell cultures from a marine sponge that produces bioactive compounds. J. Mar. Biotechnol. 4:145-149; 1996.
9. Klautau, M.; Custudio, M. R.; Borjevic, R. In vitro culture of primary cell lines from marine sponges. In: van Soest, R. W. M.; van Kempen, Th. M. G.; Braekman, J. C., ed. Sponges in time and space, Proceedings of the 4th International Porifera Congress, April 19-23, 1993; Amsterdam. Netherlands; 1994:401-406.
10. Leys, S. P. Sponge cell culture; a comparative evaluation of adhesion to a native tissue extract and other culture substrates. Tissue Cell 29:77-87; 1997.
11. McCaffrey, T. A.; Agarwal, L. A.; Weksler, B. B. A rapid fluorimetric DNA assay for the measurement of cell density and proliferation in vitro. In Vitro Cell. Dev. Biol. 24:247-252; 1988.
12. Muller, W. E.; Ugarkovic, D.; Gamulin, V.; Weiler, B.; Schroder, H. C. Intracellular signal transduction pathways in sponges. Electron Microsc. Rev. 3:97-114; 1990.
13. Pomponi, S. A.; Willoughby, R. Sponge cell culture for production of bioactive metabolites. In: van Soest, R. W. M.; van Kempen, Th. M. G.; Braekman, J. C., ed. Sponges in time and space, Proceedings of the 4th International Porifera Congress, April 19-23, 1993; Amsterdam, Netherlands; 1994:395-400.
14. Pomponi, S. A.; Willoughby, R.; Kaighn, M. E.; Wright, A. E. Development of techniques for in vitro production of bioactive natural products from marine sponges. In: Maramorosch, K.; Mitsuhashi, J., ed. Invertebrate cell culture: novel directions and biotechnology applications. Enfield, NH: Science Publishers; 1997a:231-237.
15. Pomponi, S. A.; Willoughby, R.; Kelly-Borges, M. Sponge cell culture. In: Cooksey, K. E., ed. Molecular approaches to the study of the ocean. London, UK: Chapman & Hall; 1997b:423-429.
16. Rinkevich, B. Cell cultures from marine invertebrates: obstacles, new approaches and recent improvements. J. Biotechnol. 70(1-3):133-153; 1999.
17. Rinkevich, B.; Ilan, M.; Blisko, R. Further steps in the initiation of cell cultures from embryos and adult sponge colonies. In Vitro Cell. Dev. Biol. 34:753-756; 1998.
18. Schroder, H. C.; Amberger, V.; Renneisen, K.; Bachmann, M.; Kurelec, B.; Uhlenbruck, G.; Muller, W. E. Protein kinase C phosphorylates the sponge aggregation receptor after its binding to the homologous aggregation factor. Eur. J. Cell Biol. 48:142-149; 1989.
19. Skehan, P.; Storeng, R.; Scudiero, D., et al. New colorimetric assay for anticancer-drug screening. J. Natl. Cancer Inst. 82:1107-1112; 1990.
20. Sorokin, S. J.; Pomponi, S. A.; Willoughby, R.; Armstrong, J. A.; Mikhailov, V. V. Microbial contamination and cell nutrition in sponge cell culture. In: Sponges in time and space, Book of Abstracts, 4th International Porifera Congress. University of Amsterdam, Institute of Taxonomic Zoology; April 1993:1-149.
21. Yentsch, C. M.; Pomponi, S. A. Strategies for flow cytometric analysis of marine microalgae and sponge cells, Methods Cell Biol. 42:523-538; 1994.
22. Zar, J. H. Biostatistical analysis. Englewood Cliffs, NJ: Prentice-Hall; 1974: 1-620.