Whole body protein turnover can be measured non-invasively in women using the end product method with [15N]glycine to show changes with the menstrual cycle and pregnancy

European Journal of Clinical Nutrition

Volumn 54, Issue 4, 2000, Pages 329-336

  Jackson, A A ^a   Duggleby, S L ^a   Grove, G ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

Author keywords

15N glycine; Menstruation; Pregnancy; Protein degradation; Protein synthesis; Protein turnover

Indexed keywords

AMMONIA; CARBON 13; GLYCINE N 15; HORMONE; LEUCINE; NITROGEN; ORAL CONTRACEPTIVE AGENT; PROTEIN; RADIOPHARMACEUTICAL AGENT; UNCLASSIFIED DRUG; UREA;

ADULT; ARTICLE; BODY WEIGHT; CONTROLLED STUDY; DIET; DOSE RESPONSE; FEMALE; GESTATIONAL AGE; HORMONE DETERMINATION; HUMAN; HUMAN EXPERIMENT; ISOTOPE LABELING; MENSTRUAL CYCLE; NITROGEN BALANCE; NON INVASIVE MEASUREMENT; NORMAL HUMAN; OVULATION; PREGNANCY; PROTEIN METABOLISM; PROTEIN SYNTHESIS; STANDARDIZATION; URINALYSIS; WHOLE BODY COUNTING;

ADMINISTRATION, ORAL; ADULT; BODY MASS INDEX; FEMALE; GLYCINE; HUMANS; MALE; MENSTRUATION; OVULATION; PREGNANCY; PROTEIN BIOSYNTHESIS; PROTEINS;

EID: 0034032684     PISSN: 09543007     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.ejcn.1600958     Document Type: Article

References (34)

1. Barker DJP (1998): Mothers, babies and health in later life. Churchill Livingstone: Edinburgh.
2. Bisdee JT, Garlick PJ & James WPT (1989a): Metabolic changes during the menstrual cycle. Br. J. Nutr. 61, 641-650.
3. Bisdee JT, James WPT & Shaw MA (1989b): Changes in energy expenditure during the menstrual cycle. Br. J. Nutr. 61, 187-199.
4. Calloway DH (1974): Nitrogen balance during pregnancy. In Nutrition and fetal development ed. 79-94. M Winnick Wiley: New York.
5. Calloway DH & Kurzer M (1982): Menstrual cycle and protein requirements of women. J. Nutr. 112, 356-366.
6. Crawley H (1992): Food portion sizes. HMSO: London.
7. Dalvit SP (1981): The effect of the menstrual cycle on patterns of food intake. Am. J. Clin. Nutr. 1811-1815.
8. de Benoist B, Jackson AA, Hall JStE & Persaud C (1985): Whole-body protein turnover in Jamaican women during normal pregnancy. Hum. Nutr.: Clin. Nutr. 39C, 167-179.
9. Duggleby SL & Jackson AA (1999): Whole body protein turnover in premium English women of different body mass index. Proc. Nutr. Soc. 58, 55A.
10. Duggleby SL & Jackson AA (2000): Whole body protein turnover during pregnancy, maternal body composition and fetal outcome. Proc. Nutr. Soc. (in the press).
11. Durnin JVGA & Womersley J (1974): Body fat assessed from total body density and its estimation from skinfold thickness: measurements on 481 men and women aged from 16 to 72 years. Br. J. Nutr. 32, 77-97.
12. FAO/WHO/UNU (1985): Energy and protein requirements. Report of a Joint FAO/WHO/UNU Meeting (WHO Technical Report Series No. 552). WHO: Geneva.
13. 15N]-glycine. Hum. Nutr. Clin. Nutr. 38C, 63-73.
14. Fern EB, Garlick PJ & Waterlow JC (1985a): The concept of the single body pool of metabolic nitrogen in determining the rate of whole-body nitrogen turnover. Hum. Nutr. Clin. Nutr. 39C, 85-99.
15. 15N. Clin. Sci. 271-282.
16. Godfrey K, Robinson S, Barker DJP, Osmond C & Cox V (1996): Maternal nutrition in early and late pregnancy in relation to placental and fetal growth. B. Med. J. 312, 410-414
17. 15N glycine. Ph. D. thesis, University of Southampton.
18. Grove G & Jackson AA (1995): Measurement of protein turnover in normal man using the end-product method with oral [15N]glycine: comparison of single dose and intermittent dose regimens. Br. J. Nutr. 74, 491-507.
19. Holland B, Welch AA, Unwin ID, Buss DH, Paul AA, Southgate DAT (1991): McCance and Widdowson's The Composition of Foods. Royal Society of Chemistry and Ministry of Agriculture Fisheries and Food: London.
20. Hytten FE & Leitch I (1971): The physiology of human pregnancy. Blackwell Scientific: Oxford.
21. Jackson AA (1987): Measurement of protein turnover during pregnancy. Hum. Nutr.: Clin. Nutr. 41C, 497-498.
22. Jackson AA, Golden MHN, Jahoor PF & Landman JP (1980): The isolation of urea-N and ammonia-N from biological samples for mass spectrometry. Anal. Biochem. 105, 14-17.
23. Kaplan A (1965): Urea nitrogen and ammonia nitrogen. In: Standard methods in Clinical chemistry, ed. S Meites, 245-256. Academic Press: New York.
24. Kramer MS (1987): Determinants of low birthweight: methodological assessment and meta-analysis. Bull. WHO 65, 663-737.
25. Langhoff-Roos J, Lindmark g & Gebre-Medhin M (1987): Maternal fat sotres and fat accretion during pregnancy in relation to infant birthweight. Br. J. Obstet. Gynaecol. 94, 1170-1177.
26. Mathews F, Yudkin P & Neil A (1999): Influence of maternal nutrition on outcome of pregnancy: prospective cohort study. B. Med. J. 319, 339-343.
27. Pannemans DLE, Halliday D, Westerterp KR & Kester ADM (1995): Effect of variable protein intake on whole-body protein turnover in young men and women. Am. J. Clin. Nutr. 61, 69-74.
28. Picou D & Taylor-Roberts T (1969): The measurement of total protein synthesis and catabolism and nitrogen turnover in infants in different nutritional states and receiving different amounts of dietary protein. Clin. Sci. 36, 283-296.
29. Solomon SJ, Mindy SK & Calloway DH (1982): Menstrual cycle and basal metabolic rate in women. Am. J. Clin. Nutr. 36, 611-616.
30. Thompson GN & Halliday D (1992): Protein turnover in pregnancy. Eur. J. Clin. Nutr. 46, 411-417.
31. Waterlow JC (1995): Whole-body protein turnover in humans - past, present, and future. A. Rev. Nutr. 15, 57-92.
32. 15N: comparison of end products and dose regimes. Am. J. Physiol. 235, E165-E174.
33. Watson PE, Watson ID & Batt RD (1980): Total body water volumes for adult males and females from simple anthropometric measurements. Am. J. Clin. Nutr. 33, 27-39.
34. Willommet L, Schutz Y, Whitehead R, Jequier E & Fern EB (1992): Whole body protein metabolism and resting energy expenditure in pregnant Gambian women. Am. J. Physiol. 263, E624-E631.