The Bacillus subtilis cell division protein FtsL localizes to sites of septation and interacts with DivIC

Molecular Microbiology

Volumn 36, Issue 4, 2000, Pages 846-855

  Sievers, Jörg ^a   Errington, Jeff ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; MEMBRANE PROTEIN;

ARTICLE; BACILLUS SUBTILIS; CELL DIVISION; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; SPOROGENESIS;

ANIMALS; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CELL CYCLE PROTEINS; CELL DIVISION; ESCHERICHIA COLI PROTEINS; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MEMBRANE PROTEINS; RABBITS; RECOMBINANT FUSION PROTEINS; SPORES, BACTERIAL;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS);

EID: 0034029042     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.01895.x     Document Type: Article

References (42)

1. Anagnostopoulos, C., and Spizizen, J. (1961) Requirements for transformation in Bacillus subtilis. J Bacteriol 81: 741-746.
2. Barak, I., Behari, J., Olmedo, G., Guzman, P., Brown, D.P., and Youngman, P. (1996) Structure and function of the Bacillus subtilis SpoIIE protein and its localisation at the sites of septum assembly. Mol Microbiol 19: 1047-1060.
3. Beall, B., and Lutkenhaus, J. (1989) Nucleotide sequence and insertional activation of a Bacillus subtilis gene that affects cell division, sporulation and temperature sensitivity. J Bacteriol 171: 6821-6834.
4. Beall, B., and Lutkenhaus, J. (1991) FtsZ in Bacillus subtilis is required for vegetative septation and for asymmetric septation during sporulation. Genes Dev 5: 447-455.
5. Beall, B., and Lutkenhaus, J. (1992) Impaired cell division and sporulation of a Bacillus subtilis strain with the ftsA gene deleted. J Bacteriol 174: 2398-2403.
6. Cormack, B.P., Valdivia, R.H., and Falkow, S. (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173: 33-38.
7. Daniel, R.A., and Errington, J. (2000) Intrinsic instability of the essential cell division protein FtsL of Bacillus subtilis, and a role for DivIB protein in FtsL turnover. Mol Microbiol 36: 278-289.
8. Daniel, R.A., Williams, A.M., and Errington, J. (1996) A complex four-gene operon containing essential cell division gene pbpB in Bacillus subtilis. J Bacteriol 178: 2343-2350.
9. Daniel, R.A., Harry, E.J., Katis, V.L., Wake, R.G., and Errington, J. (1998) Characterisation of the essential cell division gene ftsL (yllD) of Bacillus subtilis and its role in assembly of the division apparatus. Mol Microbiol 29: 593-604.
10. Daniel, R.A., Harry, E.J., and Errington, J. (2000) Role of penicillin-binding protein PBP 2B in assembly and functioning of the division machinery of Bacillus subtilis. Mol Microbiol 35: 299-311.
11. Erickson, H.P. (1997) FtsZ, a tubulin homologue in prokaryote cell division. Trends Cell Biol 7: 362-367.
12. Errington, J., and Mandelstam, J. (1983) Variety of sporulation phenotypes resulting from mutations in a single regulatory locus, spoIIA, in Bacillus subtilis. J Gen Microbiol 129: 2091-2101.
13. Feucht, A., Daniel, R.A., and Errington, J. (1999) Characterization of a morphological checkpoint coupling cell-specific transcription to septation in Bacillus subtilis. Mol Microbiol 33: 1015-1026.
14. Ghigo, J.-M., Weiss, D.S., Chen, J.C., Yarrow, J.C., and Beckwith, J. (1999) Localisation of FtsL to the Escherichia coli septal ring. Mol Microbiol 31: 725-737.
15. C. Mol Microbiol 10: 371-384.
16. Glaser, P., Sharpe, M.E., Raether, B., Perego, M., Ohlsen, K., and Errington, J. (1997) Dynamic, mitotic-like behavior of a bacterial protein required for accurate chromosome partitioning. Genes Dev 11: 1160-1168.
17. Guzman, L.M., Weiss, D.S., and Beckwith, J. (1997) Domain-swapping analysis of Ftsl, FtsL, and FtsQ, bitopic membrane proteins essential for cell division in Escherichia coli. J Bacteriol 179: 5094-5103.
18. Harlow, E., and Lane, D.P. (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
19. Harry, E.J., and Wake, R.G. (1997) The membrane-bound cell division protein DivIB is localised to the division site in Bacillus subtilis. Mol Microbiol 25: 275-283.
20. Harry, E.J., Stewart, B.J., and Wake, R.G. (1993) Characterization of mutations in divIB of Bacillus subtilis and cellular localization of the DivIB protein. Mol Microbiol 7: 611-621.
21. Harry, E.J., Partridge, S.R., Weiss, A.S., and Wake, R.G. (1994) Conservation of the 168 divIB gene in Bacillus subtilis W23 and B. licheniformis, and evidence for homology to ftsQ of Escherichia coli. Gene 147: 85-89.
22. Itaya, M., Kondo, K., and Tanaka, T. (1989) A neomycin resistance gene cassette selectable in a single copy state in the Bacillus subtilis chromosome. Nucleic Acids Res 17: 4410.
23. Jenkinson, H.F. (1983) Altered arrangement of proteins in the spore coat of a germination mutant of Bacillus subtilis. J Gen Microbiol 129: 1945-1958.
24. Katis, V.L., and Wake, R.G. (1999) Membrane-bound division proteins DivIB and DivIC of Bacillus subtilis function solely through their external domains in both vegetative and sporulation division. J Bacteriol 181: 2710-2718.
25. Katis, V.L., Harry, E.J., and Wake, R.G. (1997) The Bacillus subtilis division protein DivIC is a highly abundant membrane-bound protein that localises to the division site. Mol Microbiol 26: 1047-1055.
26. Kunst, F., and Rapoport, G. (1995) Salt stress is an environmental signal affecting digestive enzyme synthesis in Bacillus subtilis. J Bacteriol 177: 2403-2407.
27. Levin, P.A., and Losick, R. (1994) Characterisation of a cell division gene from Bacillus subtilis that is required for vegetative and sporulation septum formation. J Bacteriol 176: 1451-1459.
28. Levin, P.A., and Losick, R. (1996) Transcription factor Spo0A switches the localisation of the cell division protein FtsZ from a medial to a bipolar pattern. Genes Dev 10: 478-488.
29. Lewis, P.J., and Errington, J. (1997) Direct evidence for active segregation of oriC regions of the Bacillus subtilis chromosome and co-localisation with the Spo0J partitioning protein. Mol Microbiol 25: 945-954.
30. Lewis, P.J., and Marston, A.L. (1999) GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis. Gene 227: 101-109.
31. Lutkenhaus, J., and Addinall, S.G. (1997) Bacterial cell division and the Z ring. Annu Rev Biochem 66: 93-116.
32. Marston, A.L., Thomaides, H.B., Edwards, D.H., Sharpe, M.E., and Errington, J. (1998) Polar localisation of the MinD protein of Bacillus subtilis and its role in selection of the mid-cell division site. Genes Dev 12: 3419-3430.
33. Miroux, B., and Walker, J.E. (1996) Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels. J Mol Biol 260: 289-298.
34. Partridge, S.R., and Errington, J. (1993) The importance of morphological events and intercellular interactions in the regulation of prespore-specific gene expression during sporulation in Bacillus subtilis. Mol Microbiol 8: 945-955.
35. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
36. Sanchez, M., Valencia, A., Ferrandiz, M.-J., Sander, C., and Vincente, M. (1994) Correlation between the structure and biochemical activities of FtsA, an essential cell division protein of the actin family. EMBO J 13: 4919-4925.
37. Sterlini, J.M., and Mandelstam, J. (1969) Commitment to sporulation in Bacillus subtilis and its relationship to the development of actinomycin resistance. Biochem J 113: 29-37.
38. Sun, Q., and Margolin, W. (1998) FtsZ dynamics during the division cycle of live Escherichia coli cells. J Bacteriol 180: 2050-2056.
39. Thornewell, S.J., East, A.K., and Errington, J. (1993) An efficient expression and secretion system based on Bacillus subtilis phage φ105 and its use for the purification of B. cereus β-lactamase I. Gene 133: 47-53.
40. Wang, X., Huang, J., Mukherjee, A., Cao, C., and Lutkenhaus, J. (1997) Analysis of the interaction of FtsZ with itself, GTP and FtsA. J Bacteriol 179: 5551-5559.
41. Wu, L.J., and Errington, J. (1994) Bacillus subtilis SpoIIIE protein required for DNA segregation during asymmetric cell division. Science 264: 572-575.
42. Yanouri, A., Daniel, R., Errington, J., and Buchanan, C.E. (1993) Cloning and sequencing of the cell division gene pbpB which encodes penicillin-binding protein 2B in Bacillus subtilis. J Bacteriol 175: 7604-7616.