Thermal stability of human haemoglobin in the presence of sarcosine and sorbitol

Biotechnology Letters

Volumn 22, Issue 5, 2000, Pages 335-339

  Di Domenico, Roberto ^a   Lavecchia, Roberto ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

Author keywords

Haemoglobin; Osmolytes; Protein denaturation; Stabilization

Indexed keywords

HEMOGLOBIN; SARCOSINE; SORBITOL;

ARTICLE; CHEMICAL REACTION KINETICS; ERYTHROCYTE; HUMAN; PROTEIN DENATURATION; PROTEIN STABILITY; THERMOSTABILITY;

EID: 0034022033     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1005665031667     Document Type: Article

References (19)

1. Adlercreutz P, Mattiasson B (1982) Oxygen supply by hemoglobin or emulsions of perfluorochemicals. Eur. J. Appl. Microbiol. Biotechnol. 16: 165-170.
2. Antonini E, Brunori M (1971) Hemoglobin and Myoglobin in their Reactions with Ligands. Amsterdam: North Holland.
3. Back JF, Oakenfull D, Smith MB (1979) Increased thermal stability of proteins in the presence of sugars and polyols. Biochemistry 18: 5191-5196.
4. Chang TMS, ed. (1993) Blood Substitutes and Oxygen Carriers. New York: Marcel Dekker.
5. Cioci F, Lavecchia R (1997) Molecular thermodynamics of heat-induced protein unfolding in aqueous media. AIChE J. 43: 525-534.
6. Cioci F, Lavecchia R (1998a) Thermostabilization of proteins by water-miscible additives. Chem. Biochem. Eng. Q. 12: 191-199.
7. Cioci F, Lavecchia R (1998b) Sorbitol-mediated stabilization of human IgG against thermal inactivation. Biotechnol. Tech. 11: 855-858.
8. Creighton TE (1993) Proteins: Structure and Molecular Properties. New York: Freeman, pp. 293-296.
9. Gekko K (1982) Calorimetric study on thermal denaturation of lysozyme in polyol-water mixtures. J. Biochem. (Tokyo) 91: 1197-1204.
10. Hand SC, Somero GN (1982) Urea and methylamine effects on rabbit muscle phosphofructokinase. Catalytic stability and aggregation state as a function of pH and temperature. J. Biol. Chem. 257: 734-741.
11. Lumry R, Eyring H (1954) Conformation changes of proteins. J. Phys. Chem. 58: 110-120.
12. Ogden JE (1992) Recombinant haemoglobin in the development of red-blood-cell substitutes. Trends Biotechnol. 10: 91-96.
13. Olsen KW (1994) Thermal denaturation procedures for hemoglobin. Meth. Enzymol. 231: 514-524.
14. Santoro MM, Liu Y, Khan MA, Hou L, Bolen DW (1992) Increased thermal stability of proteins in the presence of naturally occurring osmolytes. Biochemistry 31: 5278-5283.
15. Thakar M, Bilenko A, Becktel WJ (1994) Osmolyte mediation of T7 DNA polymerase and plasmid DNA stability. Biochemistry 33: 12255-12259.
16. Timasheff SN, Arakawa T (1989) Stabilization of protein structure by solvents. In: TE Creighton, ed. Protein Structure: A Practical Approach. Oxford: IRL Press, pp. 331-345.
17. Winslow RM, Chapman KW (1994) Pilot-scale preparation of hemoglobin solutions. Meth. Enzymol. 231: 3-16.
18. Xie G, Timasheff SN (1997) Mechanism of stabilization of ribonuclease A by sorbitol: preferential hydration is greater for the denatured than for the native protein. Protein Sci. 6: 211-221.
19. Yancey PH, Clark ME, Hand SC, Bowlus RD, Somero GN (1982) Living with water stress: evolution of osmolyte systems. Science 217: 1214-1222.