Effect of dicyclohexylcarbodiimide (DCCD) on transport parameters in the frog cornea epithelium

Journal of Membrane Biology

Volumn 174, Issue 2, 2000, Pages 97-103

  Carrasquer, G ^a   Li, M ^a   Dinno, M A ^b  

^a University of Louisville School of Medicine   (United States)

^b EAST CAROLINA UNIVERSITY   (United States)

Author keywords

Dicyclohexylcarbodiimide; Frog cornea (R. catesbeiana); K+ conductance; Microelectrode technique; Na+ K+ ATPase; Short circuit current

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); DICYCLOHEXYLCARBODIIMIDE;

ANIMAL TISSUE; ARTICLE; CHLORIDE CONDUCTANCE; CORNEA EPITHELIUM; MEMBRANE CONDUCTANCE; MEMBRANE RESISTANCE; MEMBRANE TRANSPORT; NONHUMAN; POTASSIUM CONDUCTANCE; SHORT CIRCUIT CURRENT;

AMPHOTERICIN B; ANIMALS; BIOLOGICAL TRANSPORT; CHLORIDES; DICYCLOHEXYLCARBODIIMIDE; DOSE-RESPONSE RELATIONSHIP, DRUG; EPITHELIUM, CORNEAL; NA(+)-K(+)-EXCHANGING ATPASE; POTASSIUM; RANA CATESBEIANA; SOLUTIONS;

ANIMALIA; ANURA; RANA CATESBEIANA;

EID: 0034021990     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002320001035     Document Type: Article

References (22)

1. 3 cotransporter VI: The effect of chemical modification in cotransporter activity. J. Membrane Biol. 158:49-57
2. - transport across the isolated bullfrog cornea. Exp. Eye Res. 15:375-382
3. Candia, O.A. 1982. The active translocation of Cl and Na by the frog cornea epithelium: cotransport or separate pumps? In: Chloride Transport in Biological Membranes. J.A. Zadunaisky, editor. p. 223-242. Academic, New York
4. Candia, O.A., Bentley, P.J., Cook, P.I. 1974. Stimulation by amphotericin B of active Na transport across amphibian cornea. Am. J. Physiol. 226(6):1438-1444
5. + flux ratio in frog corneal epithelium. Am. J. Physiol. 247:C454-C461
6. Carrasquer, G., Ahn, S., Schwartz, M, Rehm, W.S. 1985. Electrogenicity of the Na-K ATPase pump in bullfrog cornea. Am. J. Physiol. 249:F185-F191
7. + in bullfrog cornea. Biochim. Biophys. Acta 900:258-266
8. + conductance in frog corneal epithelium. Biochim. Biophys. Acta 1069:181-186
9. Fillingame, R.H. 1976. Purification of the carbodiimide-reactive protein component of the ATP energy-transducing system of Escherichia coli. J. Biol. Chem. 251:6630-6637
10. Forgac, M. 1989. Structure and function of vacuolar class ATP-driven proton pumps. Physiol. Rev. 69:765-796
11. + exchanger with N,N'-diclohexylcarbo diimide (DCCD) and amiloride analogues. J. Membrane Biol. 94:253-266
12. Frizzell, R.A., Field, M., Schultz, S.G. 1979. Sodium-coupled chloride transport by epithelial tissues. Am. J. Physiol. 236:F1-F8
13. Hoare, D.G., Koshland, Jr., D.E. 1967. A method for the quantitative modification and estimation of carboxylic acid groups in proteins. J. Biol. Chem. 242:2447-2453
14. Khorana, H.G. 1953. The chemistry of carbodiimides. Chem. Rev. 53:145-166
15. Nagel, W. 1976. The intracellular electrical profile of the frog skin epithelium. Pfluegers Arch. 365:135-143
16. Nagel, W., Carrasquer, G. 1989. Effect of loop diuretics on bullfrog cornea epithelium. Am. J. Physiol. 256:C750-C755
17. Nagel, W., Reinach, P. 1980. Mechanism of stimulation by epinephrine of active transepithelial Cl transport in isolated frog cornea. J. Membrane Biol. 56:73-79
18. Reus, L., Reinach, P.S., Weinman, S.A., Grady, T.P. 1983. Intracellular ion activities and Cl transport mechanisms in bullfrog corneal epithelium. Am. J. Physiol. 244:C336-C347
19. + translocation: regional localization, kinetic characteristics, and chloride dependence. Proc. Soc. Exp. Biol. And Med. 200(4):562-570
20. Sheehan, J.C., Hess, G.P. 1955. A new method of forming peptide bonds. J. Am. Chem. Soc. 77:1067-1068
21. Ussing, H.H., Zehran, K. 1951. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin. Acta Physiol. Scand. 23:110-
22. Zadunaisky, J.A. 1972. Sodium activation of chloride transport in the frog cornea. Biochim. Biophys. Acta 282:255-257