Mutational analyses of restriction endonuclease - HindIII mutant E86K with higher activity and altered specificity

Protein Engineering

Volumn 13, Issue 4, 2000, Pages 283-289

  Tang, Dahai ^a   Ando, Shoji ^a   Takasaki, Yozo ^a   Tadano, Jutaro ^a  

^a SAGA UNIVERSITY   (Japan)

Author keywords

HindIII endonuclease; His tag sequence; Site directed mutagenesis; Star activity; Transition metal

Indexed keywords

AMINO ACID; MANGANESE; MOLYBDENUM; RESTRICTION ENDONUCLEASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; DNA BINDING; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; MUTATION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS;

ESCHERICHIA COLI;

EID: 0034020687     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/13.4.283     Document Type: Article

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