The use of flow cytometry for the detection of CMV-specific antigen (pp65) in leukocytes of kidney recipients

Clinical Transplantation

Volumn 14, Issue 2, 2000, Pages 147-151

  Essa, Sahar ^a,b   Pacsa, Alexander S ^a   Al Attiyah, Raja'a ^a   El Shazly, Amany ^a   Raghupathy, Raj ^a   Said, Tareq ^b  

^a MINISTRY OF HEALTH   (Kuwait)

^b KUWAIT UNIVERSITY   (Kuwait)

Author keywords

CMV; Flow cytometry; pp65 assay; Renal transplants

Indexed keywords

ANTIGEN PP65; AZATHIOPRINE; CYCLOSPORIN A; STEROID; UNCLASSIFIED DRUG; VIRUS ANTIGEN;

ADULT; ARTICLE; BIOASSAY; CONTROLLED STUDY; CORRELATION FUNCTION; CYTOMEGALOVIRUS INFECTION; EMBRYO; FEMALE; FIBROBLAST; FLOW CYTOMETRY; HUMAN; HUMAN CELL; KIDNEY GRAFT REJECTION; KIDNEY TRANSPLANTATION; LEUKOCYTE; MAJOR CLINICAL STUDY; MALE; PRIORITY JOURNAL; VIRUS LOAD;

ADOLESCENT; ADULT; ANTIGENS, VIRAL; CELLS, CULTURED; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; FEMALE; FIBROBLASTS; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; HUMANS; IMMUNOSUPPRESSIVE AGENTS; KIDNEY; KIDNEY TRANSPLANTATION; MALE; MIDDLE AGED; NEUTROPHILS; PHOSPHOPROTEINS; PREDICTIVE VALUE OF TESTS; VIRAL MATRIX PROTEINS;

EID: 0034020596     PISSN: 09020063     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-0012.2000.140208.x     Document Type: Article

References (17)

1. BREININ MK, ZITELLI B, STARZL T, HO M. Epstein-Barr virus, cytomegalovirus and other viral infections in children after liver transplantation. J Infect Dis 1987: 156: 27.
2. DREW WL, MILLS J, LEVVY J et al. Cytomegalovirus infection and abnormal T-lymphocyte subset ratios in homosexual men. Annu Intern Med 1985: 103: 61.
3. DUMMER JS, HARDY A, POORSATTAR A, HO M. Early infections in kidney, heart, and liver transplant recipients on cyclosporine. Transplantation 1983: 36: 259.
4. DUMMER JS, WHITE LT, HO M, GRIFFITH R, HARDESTY RL, BAHNSON HT. Morbidity of cytomegalovirus infection in recipients of heart or heart-lung transplants who received cyclosporine. J Infect Dis 1985: 152: 1182.
5. ELMENDORF S, MCSHARRY J, LAFFIN J, FOGLEMAN D, LEHMAN J. Detection of an early cytomegalovirus antigen with two-color quantitative flow cytometry. Cytometry 1988: 9: 254.
6. GRIFFITH PD. Diagnostic techniques for cytomegalovirus infection. Clin Haematol 1984: 13: 631.
7. GREENBERG SB, LINDER S, BAXTER B, PARIS E, MARCUS DM, DAESSMAN G. Lymphocyte subsets and urinary excretion of cytomegalovirus among homosexual men attending a clinic for sexually transmitted diseases. J Infect Dis 1984: 150: 330.
8. GERNA G, REVELLO MG, PERCIVALLE E, MORINI F. Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia. J Clin Microbiol 1992: 30: 1232.
9. GHISETTI V, BARBUI A, DONEGANI E et al. Comparison of polymerase chain reaction and pp65 antigen test for early detection of human cytomegalovirus in blood leukocytes of cardiac transplant recipients. Clin Microbiol Infect 1996: 1: 195.
10. LASKIN OL, STAHL-BAYLISS CM, KALMAN CM, ROSECAN LR. Use of ganciclovir to treat serious cytomegalovirus infection in patients with AIDS. J Infect Dis 1987: 155: 323.
11. MAR EC, CHENY YC, HUANG ES. Effect of 9-(1,3-dihydroxy-22-proxy-methyl) guanine on human cytomegalovirus replication in vitro. Antimicrob Agents Chemother 1983: 24: 518.
12. MCSHARRY JJ. Uses of flow cytometry in virology. Clin Microbiol Rev 1994: 7: 576.
13. PLOEM-ZAAIJAER JJ, MESKER WE, BOLAND GJ et al. Automated image cytometry for detection of rare viral antigen-positive cells in peripheral blood. Cytometry 1994: 15: 199.
14. IMPERT-MARCILLE B-M, NELLY R, ANNE-SOPHIE P et al. Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry. Clin Microbiol Infect 1997: 10: 2665.
15. EMANUEL D, PEPPARD J, STOVER D, GOLD J, ARMSTRONG D, HAMMERLING V. Rapid immunodiagnosis of cytomegalovirus pneumonia by bronchoalveolar lavage using human and murine monoclonal antibodies. Annu Intern Med 1986: 104: 476.
16. JIWA N, MESKER W, PLOEM-ZAAIJER J, VAN DROP W, THE T, RAAP A. Quantification of low frequency white blood cells expressing human cytomegalovirus antigen by image cytometry. Cytometry 1994: 16: 69.
17. LJUNGMAN P, PLOTKIN SA. Workshop on CMV disease: definitions, clinical severity scores, and new syndromes. Scand J Infect Dis 1995: Suppl. 99: 87.