A simplified CsCl protocol for lambda DNA purification: No enzymatic treatment/one phenol extraction

Genetics and Molecular Biology

Volumn 23, Issue 1, 2000, Pages 65-66

  Santelli, Roberto V ^a   Navarro Cattapan, Lud Deise ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

CESIUM CHLORIDE; PHENOL; VIRUS DNA;

ARTICLE; BACTERIOPHAGE LAMBDA; DENSITY GRADIENT CENTRIFUGATION; EXTRACTION; LIGATION; MOLECULAR CLONING; NONHUMAN; TITRIMETRY;

BACTERIOPHAGE LAMBDA;

EID: 0034019388     PISSN: 14154757     EISSN: None     Source Type: Journal    
DOI: 10.1590/S1415-47572000000100011     Document Type: Article

References (6)

1. Kaiser, K., Murray, N.E. and Wittaker, P. (1995). Construction of representative genomic DNA libraries using phage lambda replacement vectors. In: DNA Cloning 1 - A Practical Approach (Glover, D.M. and Hames, B.D., eds.). Chapter 2. IRL-Press, Oxford University Press, New York, pp. 37-82.
2. Kirby, K.S. (1957). A new method for the isolation of deoxyribonucleic acid: Evidence on the nature of bonds between deoxyribonucleic acid and protein. Biochem. J. 66: 495-504.
3. Latchman, D.S. and Brickell, P. (1986). An improved method for the isolation of high yields of bacteriophage lambda DNA. Nucleic Acids Res. 14:5220.
4. Maniatis, T., Sambrook, J. and Fritsch, E.F. (1982). Molecular Cloning - A Laboratory Manual. Cold Spring Harbor Press, Cold Spring Harbor. New York, pp. 76-85.
5. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning - A Laboratory Manual. 2nd edn. Cold Spring Harbor Press, Cold Spring Harbor, New York (2.60-2.80).
6. Schleif, R.F. and Wensink, P. (1981). Lambda DNA purification. In: Practical Methods in Molecular Biology (Manor, E.P., ed.). Springer-Verlag, New York.