The gene pvaB encodes oxidized polyvinyl alcohol hydrolase of Pseudomonas sp. strain VM15C and forms an operon with the polyvinyl alcohol dehydrogenase gene pvaA

Microbiology

Volumn 146, Issue 3, 2000, Pages 649-657

  Shimao, Masayuki ^a   Tamogami, Tsuyoshi ^a   Kishida, Shinsuke ^a   Harayama, Shigeaki ^b  

^a TOTTORI UNIVERSITY   (Japan)

^b MARINE BIOTECHNOLOGY INSTITUTE   (Japan)

Author keywords

Oxidized polyvinyl alcohol hydrolase; PQQ dependent polyvinyl alcohol dehydrogenase; Pseudomonas sp. VM15C; pva operon

Indexed keywords

BACTERIAL ENZYME; HYDROLASE; OXIDOREDUCTASE; POLYVINYL ALCOHOL; SERINE DEHYDRATASE; SIGNAL PEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; CONSENSUS SEQUENCE; DNA FLANKING REGION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PRIORITY JOURNAL; PSEUDOMONAS;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; POTATO VIRUS A; PROKARYOTA; PSEUDOMONAS; PSEUDOMONAS SP.;

EID: 0034017730     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-146-3-649     Document Type: Article

References (21)

1. Altschul, S. F., Gish, W., Miller, M., Myers, E. W. & Lipman, D. J. (1990). Basic local alignment search tool. J Mol Biol 215, 403-410.
2. Bairoch, A. & Bucher, P. (1994). PROSITE: recent developments. Nucleic Acids Res 22, 3583-3589.
3. Blout, E. R., Eager, V. W. & Silverman, D. C. (1946). Absorption spectra. III. Cyclic β-diketones. J Am Chem Soc 68, 566-571.
4. Blow, D. M. (1976). Structure and mechanism of chymotrypsin. Acc Chem Res 9, 145-152.
5. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248-254.
6. Briese, B. H., Schmidt, B. & Jendrossek, D. (1994). Pseudomonas lemoignei has five poly(hydroxyalkanoic acid) (PHA) depolymerase genes: a comparative study of bacterial and eucaryotic PHA depolymerases. J Environ Polym Degrad 2, 75-87.
7. Ferreira, L. M., Wood, T. M., Williamson, G., Faulds, C., Hazlewood, G. P., Black, G. W. & Gilbert, H. J. (1993). A modular esterase from Pseudomonas fluorescens subsp. cellulosa contains a non-catalytic cellulose-binding domain. Biochem J 294, 349-355.
8. Harley, C. B. & Reynolds, R. P. (1987). Analysis of E. coli promoter sequences. Nucleic Acids Res 15, 2343-2361.
9. Jendrossek, D., Müller, B. & Schlegel, H. G. (1993). Cloning and characterization of the poly(hydroxyalkanoic acid)-depolymerase gene locus, phaZ1, of Pseudomonas lemoignei and its gene product. Eur J Biochem 218, 701-710.
10. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
11. Messing, J. (1983). New M13 vectors for cloning. Methods Enzymol 101, 20-78.
12. Pearson, W. R. & Lipman, D. J. (1988). Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85, 2444-2448.
13. Sakazawa, C., Shimao, M., Taniguchi, Y. & Kato, H. (1981). Symbiotic utilization of polyvinyl alcohol by mixed cultures. Appl Environ Microbiol 41, 261-267.
14. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
15. Schirmer, A. & Jendrossek, D. (1994). Molecular characterization of the extracellular poly(3-hydroxyoctanoic acid) [P(3HO)] depolymerase gene of Pseudomonas fluorescens GK13 and of its gene product. J Bacteriol 176, 7065-7073.
16. Shimao, M., Yamamoto, H., Ninomiya, K., Kato, N., Adachi, O., Ameyama, M. & Sakazawa, C. (1984). Pyrroloquinoline quinone as an essential growth factor for a poly(vinyl alcohol)-degrading symbiont, Pseudomonas sp. VM15C. Agric Biol Chem 48, 2873-2876.
17. Shimao, M., Ninomiya, K., Kuno, O., Kato, N. & Sakazawa, C. (1986). Existence of a novel enzyme, pyrroloquinolme quinone-dependent polyvinyl alcohol dehydrogenase, in a bacterial symbiont, Pseudomonas sp. strain VM15C. Appl Environ Microbiol 51, 268-275.
18. Shimao, M., Tamogami, T., Nishi, K. & Harayama, S. (1996). Cloning and characterization of the gene encoding pyrroloquinoline quinonone-dependent poly(vinyl alcohol) dehydrogenase of Pseudomonas sp. strain VM15C. Biosci Biotechnol Biochem 60, 1056-1062.
19. Shine, J. & Dalgarno, L. (1975). Determinant of cistron specificity in bacterial ribosomes. Nature 254, 34-38.
20. Silverstein, R. M., Basler, G. C. & Morrill, T. C. (1991). Spectrometric Identification of Organic Compounds, 5th edn. New York: Wiley.
21. Takeshita, S., Sato, M., Toba, M., Masahashi, W. & Hashimoto-Gotoh, T. (1987). High-copy-number and low-copy-number plasmid vectors for lacZ alpha-complementation and chloramphenicol-or kanamycin-resistance selection. Gene 61, 63-74.