Flow cytometry for determination of the efficacy of contact lens disinfecting solutions against Acanthamoeba spp.

Applied and Environmental Microbiology

Volumn 66, Issue 3, 2000, Pages 1057-1061

  Borazjani, Roya N ^a,b   May, Lauren L ^a   Noble, Judith A ^a   Avery, Simon V ^a   Ahearn, Donald G ^a  

^a GEORGIA STATE UNIVERSITY   (United States)

^b BAUSCH AND LOMB   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORHEXIDINE GLUCONATE; CONTACT LENS SOLUTION; DISINFECTANT AGENT; FLUORESCEIN DIACETATE; METHYLENE BLUE; POLY(HEXAMETHYLENEBIGUANIDE); PROPIDIUM IODIDE;

ACANTHAMOEBA; ANTIMICROBIAL ACTIVITY; ARTICLE; CONTACT LENS; CONTROLLED STUDY; DRUG EFFECT; DRUG EFFICACY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; NONHUMAN;

ACANTHAMOEBA; ANIMALS; BIGUANIDES; CHLORHEXIDINE; CONTACT LENS SOLUTIONS; FLOW CYTOMETRY; METHYLENE BLUE; STAINING AND LABELING;

ACANTHAMOEBA; ACANTHAMOEBA CASTELLANII; ACANTHAMOEBA POLYPHAGA; PROTOZOA;

EID: 0034011782     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.66.3.1057-1061.2000     Document Type: Article

References (17)

1. Ahearn, D. G., and M. M. Gabriel. 1997. Contact lenses, disinfectants, and Acanthamoeba keratitis. Adv. Appl. Microbiol. 43:35-56.
2. Buck, S. L., and R. A. Rosenthall. 1996. A quantitative method to evaluate neutralizer toxicity against Acanthamoeba castellanii. Appl. Environ. Microbiol. 62:3521-3526.
3. Burger, R. M., R. J. Franco, and K. Drlica. 1994. Killing acanthamoebae with polyaminopropyl biguanide: quantitation and kinetics. Antimicrob. Agents Chemother. 38:886-888.
4. Davies, D. J. G., Y. Anthony, B. J. Meakin, S. Kilvington, and C. B. Anger. 1990. Evaluation of the anti-acanthamoebal activity of five contact lens disinfectants. Int. Contact Lens Clin. 17:14-20.
5. Hay, J., C. M. Kirkness, D. V. Seal, and P. Wright. 1994. Drug resistance and Acanthamoeba keratitis: the quest for alternative antiprotozoal chemotherapy. Eye 8:555-563.
6. Khunkitti, W., S. V. Avery, D. Lloyd, J. R. Furr, and A. D. Russell. 1997. Effects of hiocides on Acanthamoeba castellanii as measured by flow cytometry and plaque assay. J. Antimicrob. Chemother. 40:227-233.
7. Khunkitti, W., D. Lloyd, J. R. Furr, and A. D. Russell. 1998. Acanthamoeba castellanii: growth, encystment, excystment and biocide susceptibility. J. Infect. 36:43-48.
8. Kilvington, S., and D. G. White. 1994. Acanthamoeba: biology, ecology, and human disease. Rev. Med. Microbiol. 5:12-20.
9. Kirn, T. F. 1987. As the number of contact lens users increases, research seeks to determine risk factors and how best to prevent potential eye infections. JAMA 258:17-18.
10. Ledee, D. R., J. Hay, T. J. Byers, D. V. Seal, and C. M. Kirkness. 1996. Acanthamoeba griffini. Molecular characterization of a new corneal pathogen. Investig. Ophthalmol. Vis. Sci. 37:544-550.
11. Meisler, D. M., I. H. Ludwig, and I. Rutherford. 1991. Acanthamoeba and disinfection of soft contact lenses. Rev. Infect. Dis. 13:390-391.
12. Modi, P. A., W. Gresh, and K. Shih. 1995. Antimicrobial efficacy of two commercial rgp contact lens care systems. CLAO J. 21:242-246.
13. Moore, M. B., J. Ubelaker, and R. Silvany. 1985. Scanning electron microscopy of Acanthamoeba castellanii: adherence to surfaces of new and used contact lenses and to human corneal button epithelium. Rev. Infect. Dis. 13:423-424.
14. Talamo, J. D., and D. S. Larkin. 1993. Bilateral Acanthamoeba keratitis and gas-permeable contact lenses. Am. J. Ophthalmol. 116:651.
15. Tirado-Angel, J., M. M. Gabriel, L. A. Wilson, and D. G. Ahearn. 1996. Effects of polyhexamethylene biguanide and chlorhexidine on four species of Acanthamoeba in vitro. Curr. Eye Res. 15:225-228.
16. Ubelaker, J. E. 1991. Acanthamoeba spp.: opportunistic pathogens. Trans. Am. Microsc. Soc. 110:289-299.
17. Wilson, L. A., A. D. Sawant, R. B. Simmons, and D. G. Ahearn. 1990. Microbial contamination of contact lens storage cases and solutions. Am. J. Ophthalmol. 110:193-198.