Immunological detection of alkaline-diaminobenzidine-negative peroxisomes of the nematode Caenorhabditis elegans: Purification and unique pH optima of peroxisomal catalase

European Journal of Biochemistry

Volumn 267, Issue 5, 2000, Pages 1307-1312

  Togo, Summanuna H ^a   Maebuchi, Motohiro ^a   Yokota, Sadaki ^b   Bun ya, Masanori ^a   Kawahara, Akira ^a   Kamiryo, Tatsuyuki ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

^b UNIVERSITY OF YAMANASHI   (Japan)

Author keywords

3, 3' diaminobenzidine; Caenorhabditis elegans; Catalase; Peroxidase; Peroxisomes

Indexed keywords

CATALASE; DIAMINOBENZIDINE; PEROXIDASE;

ARTICLE; CAENORHABDITIS ELEGANS; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INACTIVATION; ENZYME LOCALIZATION; ENZYME PURIFICATION; IMMUNOCYTOCHEMISTRY; MEMBRANE VESICLE; NONHUMAN; NUCLEOTIDE SEQUENCE; PEROXISOME; PH; PRIORITY JOURNAL;

3,3'-DIAMINOBENZIDINE; AMINO ACID SEQUENCE; ANIMALS; CAENORHABDITIS ELEGANS; CATALASE; HYDROGEN-ION CONCENTRATION; IMMUNE SERA; MOLECULAR WEIGHT; PEROXISOMES;

CAENORHABDITIS ELEGANS; NEMATODA;

EID: 0034010963     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2000.01091.x     Document Type: Article

References (25)

1. 1. Bun-ya, M., Maebuchi, M., Hashimoto, T., Yokota, S. & Kamiryo, T. (1997) A second isoform of 3-ketoacyl-CoA thiolase found in Caenorhabditis elegans, which is similar to sterol carrier protein x but lacks the sequence of sterol carrier protein 2. Eur. J. Biochem. 245, 252-259.
2. 2. Antonenkov, V.D., Van Veldhoven, P.P., Waelkens, E. & Mannaerts, G.P. (1997) Substrate specificities of 3-oxoacyl-CoA thiolase A and sterol carrier protein 2/3-oxoacyl-CoA thiolase purified from normal rat liver peroxisomes. Sterol carrier protein 2/3-oxoacyl-CoA thiolase is involved in the metabolism of 2-methyl-branched fatty acids and bile acid intermediates. J. Biol Chem. 272, 26023-26031.
3. 3. Wanders, R.J.A., Denis, S., Wouters, F., Wirtz, K.W.A. & Seedorf, U. (1997) Sterol carrier protein x (SCPx) is a peroxisomal branchedchain β-ketothiolase specifically reacting with 3-oxo-pristanoyl-CoA: a new, unique role for SCPx in branched-chain fatty acid metabolism in peroxisomes. Biochem. Biophys. Res. Commun. 236, 565-569.
4. 4. Bun-ya, M., Maebuchi, M., Kamiryo, T., Kurosawa, T., Sato, M., Tohma, M., Jiang, L.L. & Hashimoto, T. (1998) Thiolase involved in bile acid formation. J. Biochem. (Tokyo) 123, 347-352.
5. 5. Seedorf, U., Raabe, M., Ellinghaus, P., Kannenberg, F., Fobker, M., Engel, T., Denis, S., Wouters, F., Wirtz, K.W.A., Wanders, R.J.A., Maeda, N. & Assmann, G. (1998) Defective peroxisomal catabolism of branched fatty acyl coenzyme A in mice lacking the sterol carrier protein-2/sterol carrier protein-x gene function. Genes Dev. 12, 1189-1201.
6. 6. Seedorf, U. & Assmann, G. (1991) Cloning, expression, and nucleotide sequence of rat liver sterol carrier protein 2 cDNAs. J. Biol. Chem. 266, 630-636.
7. 7. Mori, T., Tsukamoto, T., Mori, H., Tashiro, Y. & Fujiki, Y. (1991) Molecular cloning and deduced amino acid sequence of nonspecific lipid transfer protein (sterol carrier protein 2) of rat liver: a higher molecular mass (60 kDa) protein contains the primary sequence of nonspecific lipid transfer protein as its C-terminal part. Proc. Natl Acad. Sci. USA 88, 4338-4342.
8. 8. Seedorf, U., Brysch, P., Engel, T., Schrage, K. & Assmann, G. (1994) Sterol carrier protein x is peroxisomal 3-oxoacyl coenzyme A thiolase with intrinsic sterol carrier and lipid transfer activity. J. Biol. Chem. 269, 21277-21283.
9. 9. Angermüller, S. & Fahimi, H.D. (1981) Selective cytochemical localization of peroxidase, cytochrome oxidase and catalase in rat liver with 3,3′-diaminobenzidine. Histochemistry 71, 33-44.
10. 10. de Duve, C. & Baudhuin, P. (1996) Peroxisomes (microbodies and related particles). Physiol. Rev. 46, 323-357.
11. 11. Clare, D.A., Duong, M.N., Darr, D., Archibald, F. & Fridovich, I. (1984) Effects of molecular oxygen on detection of superoxide radical with nitroblue tetrazolium and on activity stains for catalase. Anal. Biochem. 140, 532-537.
12. 12. Fahimi, H.D. (1969) Cytochemical localization of peroxidatic activity of catalase in rat hepatic microbodies (peroxisomes). J. Cell. Biol. 43, 275-288.
13. 13. Vigil, E.L. (1970) Cytochemical and developmental changes in microbodies (glyoxysomes) and related organelles of castor bean endosperm. J. Cell Biol. 46, 435-454.
14. 14. Osumi, M., Miwa, N., Teranishi, Y., Tanaka, A. & Fukui, S. (1974) Ultrastructure of Candida yeasts grown on n-alkanes. Appearance of microbodies and its relationship to high catalase activity. Arch. Microbiol. 99, 181-201.
15. 15. Yokota, S., Himeno, M., Roth, J., Brada, D. & Kato, K. (1993) Formation of autophagosomes during degradation of excess peroxisomes induced by di-(2-ethylhexyl) phthalate treatment. II. Immunocytochemical analysis of early and late autophagosomes. Eur. J. Cell Biol. 62, 372-383.
16. 16. Sulston, J. & Hodgkin, J. (1988) Methods. In The Nematode Caenorhabditis Elegans (Wood, W.B., ed.), pp. 587-606. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
17. 17. Niki, T., Bun-ya, M., Hiraga, Y., Muro, Y. & Kamiryo, T. (1994) Nearstoichiometric interaction between the non-specific lipid-transfer protein of the yeast Candida tropicalis and peroxisomal acylcoenzyme A oxidase prevents the thermal denaturation of the enzyme in vitro. Yeast 10, 1467-1476.
18. 18. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
19. 19. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
20. 20. Tan, H., Bun-ya, M., Hirata, A. & Kamiryo, T. (1994) Predominant localization of non-specific lipid-transfer protein of the yeast Candida tropicalis in the matrix of peroxisomes. Yeast 10, 1065-1074.
21. 21. Adams, L.D. & Gallagher, S.R. (1995) Two-dimensional gel electrophoresis using the O'Farrell system. In Current Protocols in Molecular Biology (Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. & Struhl, K., eds), pp. 10.4.1-10.4.13. John Wiley & Sons, Chichester.
22. 22. Maebuchi, M., Togo, S.H., Yokota, S., Ghenea, S., Bun-ya, M., Kamiryo, T. & Kawahara, A. (1999) Type-II 3-oxoacyl-CoA thiolase of the nematode Caenorhabditis elegans is located in peroxisomes, highly expressed during larval stages and induced by clofibrate. Eur. J. Biochem. 264, 509-515.
23. 23. Kamiryo, T., Abe, M., Okazaki, K., Kato, S. & Shimamoto, N. (1982) Absence of DNA in peroxisomes of Candida tropicalis. J. Bacteriol. 152, 269-274.
24. 24. Herzog, V. & Fahimi, H.D. (1973) A new sensitive colorimetric assay for peroxidase using 3,3′-diaminobenzidine as hydrogen donor. Anal. Biochem. 55, 554-562.
25. 25. Taub, J., Lau, J.F., Ma, C., Hahn, J.H., Hoque, R., Rothblatt, J. & Chalfie, M. (1999) A cytosolic catalase is needed to extend adult lifespan in C. elegans daf-C and clk-I mutants. Nature 399, 162-166.