Performance of an automated system for quantification of hepatitis C virus RNA

Journal of Virological Methods

Volumn 86, Issue 1, 2000, Pages 55-60

  Afonso, Anne Marie Roque ^a   Didier, Josiane ^a   Plouvier, Elisabeth ^b   Falissard, Bruno ^c   Ferey, Marie Pierre ^a   Bogard, Marc ^b   Dussaix, Elisabeth ^a  

^a HÔPITAL PAUL BROUSSE   (France)

^b CENTRE HOSPITALIER DE MEAUX   (France)

^c INSERM   (France)

Author keywords

Automation; Hepatitis C virus; Quantitative polymerase chain reaction; Routine diagnostic laboratory

Indexed keywords

VIRUS RNA;

ARTICLE; AUTOANALYSIS; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENOTYPE; HEPATITIS C VIRUS; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SERODIAGNOSIS; TECHNIQUE;

AUTOMATION; EVALUATION STUDIES; HEPACIVIRUS; HEPATITIS C; HUMANS; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; REPRODUCIBILITY OF RESULTS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

HEPATITIS C VIRUS;

EID: 0034010376     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(99)00179-2     Document Type: Article

References (12)

1. Albaladejo, J., Alonso, R., Antinozzi, R., et al., 1998. Multicenter evaluation of the COBAS AMPLICOR HCV assay, an integrated PCR system for rapid detection of hepatitis C virus RNA in the diagnostic laboratory. J. Clin. Microbiol. 36, 862-865.
2. Colucci, G., Gutekunst, K., 1997. Development of a quantitative PCR assay for monitoring HCV viraemia levels in patients with chronic hepatitis C. J. Viral Hepatitis. 4 (Suppl. 1), 00-00.
3. Damen, M., Cuypers, H.T.M., Zaaijer, H.L., et al., 1996. International collaborative study on the second EUROHEP HCV-RNA reference panel. J. Virol. Methods 58, 175-185.
4. DiDomenico, N., Link, H., Knobel, R., Caratsch, T., Weschler, W., Loewy, Z.G., Rosenstrauss, M., 1996. COBAS AMPLICOR: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR. Clin. Chem. 42, 1915-1923.
5. Doglio, A., Laffont, C., Caroli-Bosc, F.X., Rochet, P., Lefebvre, J.C., 1999. Second generation of the automated COBAS AMPLICOR HCV assay improves sensitivity of hepatitis C virus RNA detection and yields results that are more clinically relevant. J. Clin. Microbiol. 37, 1567-1569.
6. Hawkins, A., Davidson, F., Simmonds, P., 1997. Comparison of plasma viras loads among individuals infected with hepatitis C virus (HCV) genotypes 1, 2, and 3 by quantiplex HCV RNA assay versions 1 and 2, Roche MONITOR assay, and in-house limiting dilution method. J. Clin. Microbiol. 35, 187-192.
7. Kessler, H.H., Dragon, E.A., Pierer, K., Santner, B.I., Liao, Y., Stünzner, D., Stelzl, E., Marth, E., 1997. Performance of the automated COBAS AMPLICOR system for the detection of hepatitis C virus RNA. Clin. Diagn. Virol. 7, 139-145.
8. Lunel, F., Cresta, P., Vitour, D., et al., 1999. Comparative evaluation of hepaitis C virus RNA quantification by branched DNA, NASBA, and MONITOR assays. Hepatology 29, 528-535.
9. Martinot-Peignoux, M., Boyer, N., Pouteau, M., et al., 1998. Predictors of sustained response to alpha interferon therapy in chronic hepatitis C. J. Hepatol. 29, 214-223.
10. Tong, C.Y., Hollingsworth, R.C., Williams, H., Irving, W.L., Gilmore, I.T., 1998. Effects of genotypes on the quantification of hepatitis C virus (HCV) RNA in clinical samples using the Amplicor HCV MONITOR Test and the Quantiplex HCV RNA 2.0 assay (bDNA). J. Med. Virol. 55, 191-196.
11. Shiratori, Y., Kato, N., Yokosuka, O., et al., 1997. Predictors of the efficacy of interferon therapy in chronic hepatitis C virus infection. Gastroenterology 113, 558-566.
12. Young, K.K.Y., Resnick, R.M., Myers, T.W., 1993. Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay. J. Clin. Microbiol. 31, 882-886.