Rainbow trout (Oncorhynchus mykiss) cystatin C: Expression in Escherichia coli and properties of the recombinant protease inhibitor

Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

Volumn 125, Issue 4, 2000, Pages 493-502

  Li, Fugen ^a   An, Haejung ^b   Seymour, Thomas A ^b   Barnes, David W ^c  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b OREGON STATE UNIVERSITY   (United States)

^c AMERICAN TYPE CULTURE COLLECTION   (United States)

Author keywords

Bacterial expression; Cystatin; Disulfide linkage; Protease; Protease inhibitor; Protein refolding; Salmonid; Trout

Indexed keywords

CYSTATIN C; PROTEINASE INHIBITOR;

ARTICLE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; RAINBOW TROUT;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GALLUS GALLUS; MYKISS; ONCORHYNCHUS MYKISS; SALMONIDAE;

EID: 0034007099     PISSN: 03050491     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0305-0491(00)00156-5     Document Type: Article

References (35)

1. Abrahamson, M., 1994. Cystatins. Methods Enzymol. 244, 685-700.
2. Abrahamson, M., Grubb, A., Olafsson, I., Lundwall, A., 1987a. Molecular cloning and sequence analysis of cDNA coding for the precursor of the human cysteine proteinase inhibitor cystatin C. FEBS Lett. 216, 229-233.
3. Abrahamson, M., Ritonja, A., Brown, M.A., Grubb, A., Machleidt, W., Barrett, A.J., 1987b. Identification of the probable inhibitory reactive sites for the cysteine proteinase inhibitors human cystatin C and chicken cystatin. J. Biol. Chem. 262, 9688-9694.
4. Abrahamson, M., Dalboge, H., Olafsson, I., Carlsen, S., Grubb, A., 1988. Efficient production of native, biologically active human cystatin C by Escherichia coli. FEBS Lett. 236, 14-18.
5. Allison, R.D., Purich, D.L., 1979. Practical consideration in the design of initial velocity enzyme rate assays. Methods Enzymol. 63, 3-22.
6. Auerswald, E.A., Genenger, G., Assfalg-Machleidt, I., Kos, J., Bode, W., 1989. Synthesis a (desSer1 Ile29 Leu89) chicken cystatin gene, expression in E. coli as fusion protein and its isolation. FEBS Lett. 243, 186-192.
7. Auerswald, E.A., Genenger, G., Mentele, R., Lenzen, S., Assfalg-Machleid, I., Mitschang, L., Oschkinat, H., Fritz, H., 1991. Purification and characterization of a chicken egg white cystatin variant expressed in an Escherichia coli pIN-III-ompA system. Eur. J. Biochem. 200, 131-138.
8. Barrett, A.J., 1972. A new assay for cathepsin B1 and other thiol proteinases. Anal. Biochem. 47, 280-293.
9. Barrett, A.J., Kirschre, H., 1981. Cathepsin B, cathepsin H, and cathepsin L. Methods Enzymol. 80, 535-539.
10. Barrett, A.J., Kembhavi, A.A., Brown, M.A., Kirschke, H., Knight, C.G., Tamai, M., Hanada, K., 1982. L-trans-Epoxysuccinyl-leucylamido(4-guanidino)butane (E-64) and its analogs as inhibitors of cysteine proteinases including cathepsins B, H and L. Biochem. J. 201, 189-198.
11. Bessette, P.H., Aslund, F., Beckwith, J., Georgiou, G., 1999. Efficient folding of proteins with multiple disulfide bonds in the Eschcrichia coli cytoplasm. Proc. Natl. Acad. Sci. USA 96, 13703-13708.
12. 2-microglobulin in rat brain. Eur. J. Biochem. 186, 35-42.
13. Colella, R., Sakaguchi, Y., Nagase, H., Bird, J.W.C., 1989. Chicken egg white cystatin-molecular cloning, nucleotide sequence, and tissue distribution. J. Biol. Chem. 264, 17164-17169.
14. Collodi, P., Kamei, Y., Sharps, A., Weber, D., Barnes, D., 1992. Fish embryo cell cultures for derivation of stem cells and transgenic chimeras. Mol. Mar. Biol. Biotech. 1, 257-265.
15. Dalboge, H., Jensen, E.B., Tottrup, H., Grubb, A., Abrahamson, M., Olafsson, I., Carlsen, S., 1989. High-level expression of active human cystatin C in Escherichia coli. Gene 79, 325-332.
16. Freije, J.P., Balbin, M., Abrahamson, M., Velasco, G., Dalboge, H., Grubb, A., Lopez-Otin, C., 1993. Human cystatin D: cDNA cloning, characterization of the Escherichia coli expressed inhibitor, and identification of the native protein in saliva. J. Biol. Chem. 268, 15737-15744.
17. Garcia-Carreno, F.L., Dimes, L.E., Haard, N.F., 1993. Substrate-gel electrophoresis for composition and molecular weight of proteinases or proteinaceous proteinase inhibitors. Anal. Biochem. 214, 65-69.
18. Gong, Z., Yan, T., Liao, J., Lee, S.E., He, J., Hew, C.L., 1997. ZF-A367 zebrafish adult danio rerio cDNA 5′ end similar to cystatin. GenBank, accession no. AA566881.
19. Hakansson, K., Huh, C., Grubb, A., Karlsson, S., Abrahamson, M., 1996. Mouse and rat cystatin C: Escherichia coli production, characterization and tissue distribution. Comp. Biochem. Physiol. B 114, 303-311.
20. Isemura, S., Saitoh, E., Sanada, K., Isemura, M., Ito, S., 1986. Cystatin S and the related cysteine proteinase inhibitors in human saliva. In: Turk, V. (Ed.), Cystatin Proteinases and Their Inhibitors. Walter de Gruyter, Berlin, pp. 497-505.
21. Izquierdo-Pulido, M.L., Haard, T.A., Hung, J., Haard, N.F., 1994. Oryzacystatin and other proteinase inhibitors in rice grain: potential use as a fish processing aid. J. Agric. Food Chem. 42, 616-622.
22. Kaiser, C.A., Preuss, D., Grisafi, P., Botstein, D., 1987. Many random sequences functionally replace the secretion signal sequence of yeast invertase. Science 235, 312-315.
23. Koide, Y., Noso, T., 1994. The complete amino acid sequence of pituitary cystatin from chum salmon. Biosci. Biotech. Biochem. 58, 164-169.
24. Kos, J., Dolinar, M., Turk, V., 1986. Isolation and characterization of chicken egg white low-Mr kininogen. In: Turk, V. (Ed.), Cysteine Proteinases and Their Inhibitors. Walter de Gruyter, Berlin, pp. 583-591.
25. Li, F., An, H., Seymour, T.A., Morrissey, M.T., Barnes, W.D., 1995. Oncorhynchus mykiss cystatin mRNA, complete cds. GenBank, accession no. U33555.
26. Li, F., An, H., Seymour, T.A., Bradford, C.S., Morrissey, M.T., Bailey, G.S., Barnes, D.W., 1998. Molecular cloning, sequence analysis and expression distribution of rainbow trout (Oncorhynchus mykiss) cystatin C. J. Comp. Biochem. Physiol. B 121, 135-143.
27. Lindahl, P., Abrahamson, M., Bjork, I., 1992. Interaction of recombinant human cystatin C with the cysteine proteinase papain and actinidin. Biochem. J. 281, 49-55.
28. Morrissey, M.T., Wu, J.W., Lin, D.D., An, H., 1993. Effect of food grade protease inhibitors on autolysis and gel strength of surimi. J. Food Sci. 58, 1050-1054.
29. Nicklin, M.J.H., Barrett, A.J., 1984. Inhibition of cysteine proteinases and dipeptidyl peptidase I by egg-white cystatin. Biochem. J. 223, 245-253.
30. Seymour, T.A., Morrissey, M.T., Peter, M.Y., An, H., 1994. Purification and characterization of whiting proteases. J. Agric. Food Chem. 42, 2421-2427.
31. Solem, M.L., Rawson, C., Lindburg, K., Barnes, D.W., 1990. Transforming growth factor b regulates cystatin C in serum-free mouse embryo (SFME) cells. Biochem. Biophys. Res. Commun. 172, 945-951.
32. The QIAexpressionist, 1992. The High Level Expression and Protein Purification System, pp. 45-46.
33. Tsai, Y.J., Chang, G.D., Huang, C.J., Chang, Y.S., Huang, F.L., 1996. Purification and molecular cloning of carp ovarian cystatin. Comp. Biochem. Physiol. B 113, 573-580.
34. Yamashita, M., Konagaya, S., 1991. Cysteine protease inhibitor in egg of chum salmon. J. Biochem. 110, 762-766.
35. Yamashita, M., Konagaya, S., 1996. Molecular cloning and gene expression of chum salmon cystatin. J. Biochem. 120, 483-487.