Improving protein crystal quality by decoupling nucleation and growth in vapor diffusion

Protein Science

Volumn 9, Issue 4, 2000, Pages 755-757

  Saridakis, Emmanuel ^a   Chayen, Naomi E ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Crystallogenesis; Equilibration kinetics; Hanging drop; Nucleation; Protein crystallization; Vapor diffusion

Indexed keywords

HUMAN SERUM ALBUMIN; LYSOZYME; PROTEIN; THAUMATIN;

ARTICLE; CRYSTALLIZATION; DIFFUSION; PRIORITY JOURNAL; PROTEIN CONFORMATION; VAPOR;

EID: 0034006471     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.9.4.755     Document Type: Article

References (10)

1. Carter DC, Chang B, Ho JX, Keeling K, Krishnasami Z. 1994. Preliminary crystallographic studies of four crystal forms of serum albumin. Eur J Biochem 226:1049-1052.
2. Curry S, Mandelkow H, Brick P, Franks N. 1998. Crystal structure of human serum albumin complexed with fatty acids reveals an asymmetric distribution of binding sites. Nat Struct Biol 5:827-835.
3. He XM, Carter DC. 1992. Atomic structure and chemistry of human serum albumin. Nature 358:209-215.
4. Luft JR, DeTitta GT. 1995. Chaperone salts, polyethylene glycol and rates of equilibration in vapor-diffusion crystallization. Acta Crystallogr D51:780-785.
5. Mikol V, Giege R. 1992. The physical chemistry of protein crystallization. In: Ducruix A, Giege R, eds. Crystallisation of nucleic acids and proteins. New York: Oxford University Press. pp 219-239.
6. Mikol V, Rodeau J-L, Giege R. 1990. Experimental determination of water equilibration rates in the hanging drop method of protein crystallization. Anal Biochem 186:332-339.
7. Przybylska M. 1989. A double cell for controlling nucleation and growth of protein crystals. J Appl Cryst 22:115-118.
8. 2. Acta Crystallogr D50:293-297.
9. Stura EA. 1999. Seeding. In: Bergfors TM, ed. Protein crystallization: Techniques, strategies, and tips. La Jolla, California: International University Line. pp 141-153.
10. Stura EA, Wilson IA. 1992. Seeding techniques. In: Ducruix A, Giege R, eds. Crystallisation of nucleic acids and proteins. New York: Oxford University Press. pp 99-126.