Functional expression in Escherichia coli and membrane topology of porin HopE, a member of a large family of conserved proteins in Helicobacter pylori

Journal of Bacteriology

Volumn 182, Issue 9, 2000, Pages 2370-2375

  Bina, Jim ^a   Bains, Manjeet ^a   Hancock, Robert E W ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

OUTER MEMBRANE PROTEIN; PORIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; ESCHERICHIA COLI; GENE CONSTRUCT; GENE INACTIVATION; GENE INSERTION; GENETIC CONSERVATION; HELICOBACTER PYLORI; MEMBRANE MODEL; MEMBRANE STRUCTURE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; HELICOBACTER PYLORI; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; PORINS;

ESCHERICHIA COLI; HELICOBACTER PYLORI;

EID: 0034004724     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.9.2370-2375.2000     Document Type: Article

References (21)

1. Agterberg, M., H. Adsriaanse, E. Tijhaar, A. Resnick, and J. Tommassen. 1989. Role of the cell surface-exposed regions of outer membrane protein PhoE of Escherichia coli K-12 in the biogenesis of the protein. Europ. J. Biochem. 185:365-370.
2. Aim, R. A., L. S. Ling, D. T. Moir, B. L. King, E. D. Brown, P. C. Doig, D. R. Smith, B. Noonan, B. C. Guild, B. L. dejonge, G. Carmel, P. J. Tummino, A. Caruso, M. Uria-Nickelsen, D. M. Mills, C. Ives, R. Gibson, D. Merberg, S. D. Mills, Q. Jiang, D. E. Taylor, G. F. Vovis, and T. J. Trust. 1999. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397:176-180.
3. Bosch, D., and J. Tommassen. 1987. Effects of linker insertions on the biogenesis and functioning of the Escherichia coli outer membrane pore protein PhoE. Mol. Gen. Genet. 208:485-489.
4. Cowan, S. W., T. Schirmer, G. Rummel, M. Steiert, R. Ghosh, R. A. Pauptit, J. N. Jansonius, and J. P. Rosenbusch. 1992. Crystal structures explain functional properties of two E. coli porins. Nature 358:727-733.
5. Cowan, S. W., R. M. Garavito, J. N. Jansonius, J. A. Jenkins, R. Karlsson, N. König, E. F. Pai, R. A. Pauptit, P. J. Rizkallah, and J. P. Rosenbusch. 1995. The structure of OmpF porin in a tetragonal crystal form. Structure 3:1041-1050.
6. Doig, P., J. W. Austin, M. Kostrzynska, and T. J. Trust. 1992. Production of a conserved adhesin by the human gastroduodenal pathogen Helicobacter pylori. J. Bacteriol. 174:2539-2547.
7. Doig, P., and T. J. Trust. 1994. Identification of surface-exposed outer membrane antigens of Helicobacter pylori. Infect. Immun. 62:4526-4533.
8. Doig, P., M. M. Exner, R. E. Hancock, and T. J. Trust. 1995. Isolation and characterization of a conserved porin protein from Helicobacter pylori. J. Bacteriol. 177:5447-5452.
9. Exner, M. M., P. Doig, T. J. Trust, and R. E. Hancock. 1995. Isolation and characterization of a family of porin proteins from Helicobacter pylori. Infect. Immun. 63:1567-1572.
10. Hancock, R. E. W., R. Alm, J. Bina, and T. Trust. 1998. Helicobacter pylori: a surprisingly conserved bacterium Nat. Biotechnol. 16:216-217.
11. Hancock, R. E. W., and A. M. Carey. 1979. Outer membrane of Pseudomonas aeruginosa. Heat-and 2-mercaptoethanol-modifiable proteins. J. Bacteriol. 140:902-910.
12. 26. FEMS Microbiol. Lett. 6:147-150.
13. Huang, H. 1995. Molecular studies of the structure and function of Pseudomonas aeruginosa OprD: an imipenem specific porin. Ph.D. thesis. University of British Columbia, Vancouver, British Columbia, Canada.
14. Huang, H., D. Jeanteur, F. Pattus, and R. E. W. Hancock. 1995. Membrane topology and site-specilic mutagenesis of Pseudomonas aeruginosa porin OprD. Mol. Microhiol. 16:931-941.
15. Huang, H., and R. E. W. Hancock. 1996. The role of specific surface loop regions in determining the function of the imipenem-specific pore protein OprD of Pseudomonas aeruginoa. J. Bacteriol. 178:3085-3090.
16. Ilver, D., A. Arnqvist, J. Ogren, I. M. Frick, D. Kersulyte, E. T. Incecik, D. E. Berg, A. Covacci, L. Engstrand, and T. Borén. 1998. Helicobacter pylori adhesin binding fucosylated histo-hlood group antigens revealed by retagging. Science 279:373-377.
17. Jeanteur, D., J. H. Lakey, and F. Pattus. 1991. The bacterial porin superfamily: sequence alignment and structure prediction. Mol. Microbiol. 5:2153-2164.
18. Pauptit, R. A., T. Schirmer, J. N. Jansonius, J. P. Rosenbusch, M. W. Parker, A. D. Tucker, D. Tsernoglou, M. S. Weiss, and G. E. Schultz. 1991. A common channel-forming motif in evolutionarily distant porins. J. Struct. Biol. 107:136-145.
19. Rawling, E. G., F. S. L. Brinkman, and R. E. W. Hancock. 1998. Roles of the carboxy-terminal half of Pseudomonas aeruginosa major outer membrane protein OprF in cell shape, growth in low-osmolarity medium, and peptidoglycan association. J. Bacteriol, 180:3556-3562.
20. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
21. Tomb, J. F., O. White, A. R. Kerlavage, R. A. Clayton, G. G. Sutton, R. D. Fleischmann, K. A. Kelchum, H. P. Klenk, S. Gill, B. A. Dougherty, K. Nelson, J. Quackenbush, L. Zhou, E. F. Kirkness, S. Peterson, B. Loftus, D. Richardson, R. Dodson, H. G. Khalak, A. Glodek, K. McKenney. L. M. Fitzgerald, N. Lee, M. D. Adams, and J. C. Venter. 1997. The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388: 539-547.