Evidence for an RNA-based catalytic mechanism in eukaryotic nuclear ribonuclease P

RNA

Volumn 6, Issue 4, 2000, Pages 554-562

  Thomas, Brian C ^a,c   Chamberlain, Joel ^b   Engelke, David R ^b   Gegenheimer, Peter ^a  

^a UNIVERSITY OF KANSAS   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Catalytic mechanism; Enzyme; Magnesium ion; Ribonuclease P; Ribozyme; Thiosubstitution; tRNA processing; Yeast

Indexed keywords

MAGNESIUM ION; RIBONUCLEASE P; RIBONUCLEOPROTEIN; RNA; TRANSFER RNA;

ARTICLE; CATALYSIS; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; RNA CLEAVAGE; RNA PROCESSING; SACCHAROMYCES CEREVISIAE; YEAST;

BASE SEQUENCE; CATALYSIS; CELL NUCLEUS; CHLORIDES; ENDORIBONUCLEASES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; EUKARYOTIC CELLS; MAGNESIUM; MANGANESE COMPOUNDS; MUTATION; NUCLEIC ACID CONFORMATION; ORGANOTHIOPHOSPHORUS COMPOUNDS; OXYGEN; RIBONUCLEASE P; RNA PRECURSORS; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, CATALYTIC; RNA, TRANSFER, PHE; SACCHAROMYCES CEREVISIAE; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); EUKARYOTA; SACCHAROMYCES CEREVISIAE;

EID: 0034004463     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838200991477     Document Type: Article

References (27)

1. Asp. Biochemistry 35:10493-10505.
2. Chamberlain JR, Lee Y, Lane WS, Engelke DR. 1998. Purification and characterization of the nuclear RNase P holoenzyme complex reveals extensive subunit overlap with RNase MRP. Genes & Dev 12:1678-1690.
3. Chamberlain JR, Pagan-Ramos E, Kindelberger DW, Engelke DR. 1996. An RNase P RNA subunit mutation affects ribosomal RNA processing. Nucleic Acids Res 24:3158-3166.
4. Chen JL, Pace NR. 1997. Identification of the universally conserved core of ribonuclease P RNA. RNA 3:557-560.
5. P oxygen of the scissile bond. Biochemistry 36:2425-2438.
6. Frank DN, Pace NR. 1998. Ribonuclease P: Unity and diversity in a tRNA processing ribozyme. Annu Rev Biochem 67:153-180.
7. Gardiner KJ, Marsh TL, Pace NR. 1985. Ion dependence of the Bacillus subtilis RNase P reaction. J Biol Chem 260:5415-5419.
8. Guerrier-Takada C, Gardiner KJ, Marsh TL, Pace NR, Altman S. 1983. The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme. Cell 35:849-857.
9. Guerrier-Takada C, Haydock K, Allen L, Altman S. 1986. Metal ion requirements and other aspects of the reaction catalyzed by M1 RNA, the RNA subunit of ribonuclease P from E. coli. Biochemistry 25:1509-1515.
10. Jarrous N, Eder PS, Guerrier-Takada C, Hoog C, Altman S. 1998. Autoantigenic properties of some protein subunits of catalytically active complexes of human ribonuclease P. RNA 4:407-417.
11. Asp. Biochemistry 37:2393-2400.
12. Lee JY, Engelke DR. 1989. Partial characterization of an RNA component that copurifies with Saccharomyces cerevisiae RNase P. Mol Cell Biol 9:2536-2543.
13. Lee JY, Rohlman CE, Molony LA, Engelke DR. 1991. Characterization of RPR1, an essential gene encoding the RNA component of Saccharomyces cerevisiae nuclear RNase P. Mol Cell Biol 11: 721-730.
14. 2+ ions in the E. coli RNase P reaction. Ph.D. Dissertation. University of Kansas-Lawrence, Lawrence, Kansas.
15. 2+ complexes of adenine nucleotides and thionucleotides and rate constants for formation and dissociation of MgATP and MgADP. Biochemistry 23:5262-5271.
16. Piccirilli JA, Vyle JS, Caruthers MH, Cech TR. 1993. Metal ion catalysis by the Tetrahymena ribozyme. Nature 361:85-88.
17. Pitulle C, Garcia-Paris M, Zamudio KR, Pace NR. 1998. Comparative structure analysis of vertebrate ribonuclease P RNA. Nucleic Acids Res 26:3333-3339.
18. Reich C, Olsen GJ, Pace B, Pace N. 1988. Role of the protein moiety of ribonuclease P, a ribonucleoprotein enzyme. Science 239:178-181.
19. Sampson JR, Uhlenbeck OC. 1988. Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro. Proc Natl Acad Sci USA 85:1033-1037.
20. Smith D, Burgin AB, Haas ES, Pace NR. 1992. Influence of metal ions on the ribonuclease P reaction. Distinguishing substrate binding from catalysis. J Biol Chem 267:2429-2436.
21. Tallsjo A, Kirsebom LA. 1993. Product release is a rate-limiting step during cleavage by the catalytic RNA subunit of Escherichia coli RNase P. Nucleic Acids Res 21:51-57. [published erratum appears in Nucleic Acids Res 21:1686]
22. Thomas BC, Li X, Gegenheimer P. 2000. Chloroplast ribonuclease P does not utilize the ribozyme-type pre-tRNA cleavage mechanism. RNA 6:545-553.
23. Tranguch AJ, Engelke DR. 1993. Comparative structural analysis of nuclear RNase P RNAs from yeast. J Biol Chem 268:14045-14055.
24. 32P-labeled RNA: Double digestion and pancreatic ribonuclease analysis. Anal Biochem 83:228-239.
25. 32P-labeled bacteriophage MS2 RNA by a mini-fingerprinting procedure. Anal Biochem 72:433-446.
26. Wang MJ, Davis NW, Gegenheimer PA. 1988. Novel mechanisms for maturation of chloroplast transfer RNA precursors. EMBO J 7:1567-1574.
27. 2+-ribozyme by a single Rp-phosphorothioate modification in the precursor tRNA at the RNase P cleavage site. Proc Natl Acad Sci USA 93:8924-8928.