Factor analysis of confocal image sequences of human papillomavirus DNA revealed with Fast Red in cervical tissue sections stained with TOTO-iodide

Analytical and Quantitative Cytology and Histology

Volumn 22, Issue 2, 2000, Pages 168-174

  Kahn, Edmond ^a   Lizard, Gérard ^b   Frouin, Frédérique ^b   Roignot, Patrick ^b   Chardonnet, Yvette ^b   Di Paola, Robert ^b  

^a PITIÉ SALPÊTRIÈRE HOSPITAL   (France)

^b NONE

Author keywords

Cervix neoplasms; Confocal; Factor analysis; Fast Red; Laser scanning; Microscopy; Papillomavirus, human

Indexed keywords

IODINE; VIRUS DNA;

ARTICLE; CELL NUCLEUS; CONFOCAL LASER MICROSCOPY; DNA PROBE; DNA SEQUENCE; FACTORIAL ANALYSIS; FLUORESCENCE IN SITU HYBRIDIZATION; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL; UTERINE CERVIX TUMOR; WART VIRUS;

AZO COMPOUNDS; BIOPSY; CELL NUCLEUS; CERVICAL INTRAEPITHELIAL NEOPLASIA; CERVIX UTERI; DNA, VIRAL; FACTOR ANALYSIS, STATISTICAL; FEMALE; FLUORESCENT DYES; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; IN SITU HYBRIDIZATION, FLUORESCENCE; MICROSCOPY, CONFOCAL; PAPILLOMAVIRIDAE; QUINOLINIUM COMPOUNDS; THIAZOLES; UTERINE CERVICAL NEOPLASMS;

ANIMALIA; DNA VIRUSES; HUMAN PAPILLOMAVIRUS; HUMAN PAPILLOMAVIRUS TYPES; PAPILLOMAVIRUS; TUMOR VIRUS;

EID: 0034003007     PISSN: 08846812     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Butor C, Duquenne O, Mignon GK, Mogin C, Guillet JG: Solid phase cytometry allows rapid in situ quantification of human papillomavirus infection in biopsy material. Cytometry 1997;29:292-297
2. Di Paola R, Bazin JP, Aubry F, Aurengo A, Cavaillolès F, Herry JY, Kahn E: Handling of dynamic sequences in nuclear medecine. IEEE Trans Nucl Sci 1982;29:1310-1321
3. Frouin F, Cinotti L, Benali H, Buvat I, Bazin JP, Millet P, Di Paola R: Extraction of functional volumes from medical dynamic volumetric datasets. Comput Med Imaging Graph 1993;17:397-404
4. Harman HH: Modern Factor Analysis. Chicago, University of Chicago Press, 1960
5. Kahn E, Frouin F, Hotmar J, Di Paola R, Bernheim A: Confocal fluorescence analysis of the depth and focus of cytogenetic preparations. Analyt Quant Cytol Histol 1997;19: 404-412
6. Kahn E, Hotmar J, Frouin F, Di Paola M, Bazin JP, Di Paola R, Bernheim A: Spectral and dynamic confocal fluorescence characterization of cytogenetic preparations. Anal Cell Pathol 1996;12:45-54
7. Kahn E, Lizard G, Frouin F, Roignot P, Chardonnet Y, Di Paola R: Laser scanning confocal microscopy and factor analysis of biomedical image sequences (FAMIS) to detect and characterise HPV DNA sequences by FISH in HeLa cells. Cytometry 1997;28:269-279
8. Kahn E, Lizard G, Pélégrini M, Frouin F, Roignot P, Chardonnet Y, Di Paola R: Four-dimensional factor analysis of confocal image sequences (4D-FAMIS) to detect and characterise low copy numbers of human papillomavirus DNA by FISH in HeLa and SiHa cells. J Microsc 1999;193:227-243
9. Lizard G, Chignol MC, Chardonnet Y, Souchier C, Bordes M, Schmitt D, Revillard JP: Detection of human papillomavirus DNA in CaSki and HeLa cells by fluorescent in situ hybridization: Analysis by flow cytometry and confocal laser scanning microscopy. J Immunol Methods 1993;157:31-38
10. Lizard G, Chignol MC, Souchier C, Chardonnet Y, Schmitt D: Combination of laser scanning confocal microscopy improves detection of human papillomavirus DNA revealed by fluorescence in situ hybridization. Histochemistry 1994; 101:303-310
11. Lizard G, Chignol MC, Souchier C, Roignot P, Chardonnet Y, Schmitt D: Detection of low copy numbers of HPV DNA by fluorescent in situ hybridization combined with confocal microscopy as an alternative to in situ polymerase chain reaction. J Virol Methods 1998;72:15-25
12. Lizard G, Usson Y, Chignol MC, Chardonnet Y: Improvement in visualisation and localisation of human papillomavirus DNA in CaSki cells by fluorescence in situ hybridisation, laser scanning confocal microscopy and three dimensional image reconstruction. Anal Cell Pathol 1994;7: 53-61
13. Oldenbourg R, Terada H, Tiberios R, Inoué S: Image sharpness and contrast transfer in coherent confocal microscopy. J Microsc 1993;172:31-39
14. Poljak M, Seme K, Gale N: Detection of human papillomavirus in tissue specimens. Adv Anat Pathol 1998;5: 216-234
15. Raap AK: Localization properties of fluorescence cytochemical enzyme procedures. Histochemistry 1986;84:317-321
16. Rose A, Weimer P: Physical limits to the performance of imaging systems. Physics Today 1989;42:24-32
17. Shotton DM: Electronic Light Microscopy: Techniques in Modern Biomedical Microscopy. New York, Wiley-Liss, Inc, 1993
18. Speel EJM, Schutte B, Ramaekers FCS, Hopman HN: The effect of avidin-biotin interactions in detection systems for in situ hybridization. J Histochem Cytochem 1992;40:135-151
19. Speel EJM, Schutte B, Wiegant J, Ramaekers FCS, Hopman AHN: A novel fluorescence detection method for in situ hybridization, based on the alkaline phosphatase-Fast Red reaction. J Histochem Cytochem 1992;40:1299-1308
20. Suzuki T, Fujikura K, Higashiyama T, Takata K: DNA staining for fluorescence and laser confocal microscopy. J Histochem Cytochem 1997;45:49-53
21. Tekola P, Baak JPA, Beliën JAM, Bruggle J: Highly sensitive, specific, and stable new fluorescent DNA stains for confocal laser microscopy and image processing of normal paraffin sections. Cytometry 1994;17:191-195