Reversible protection of disulfide bonds followed by oxidative folding render recombinant hCGβ highly immunogenic

Vaccine

Volumn 18, Issue 17, 2000, Pages 1802-1810

  Mukhopadhyay, Asok ^a  

^a NATIONAL INSTITUTE OF IMMUNOLOGY   (India)

Author keywords

Immunogenicity; Recombinant CG ; Refolding

Indexed keywords

CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN VACCINE;

ACTIVE IMMUNIZATION; ANIMAL CELL; ANIMAL EXPERIMENT; ANTIGEN PRESENTATION; ARTICLE; DISULFIDE BOND; FEMALE; IMMUNOCONTRACEPTION; IMMUNOGENICITY; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; ANTIBODIES; CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN; CONTRACEPTION, IMMUNOLOGIC; CYSTAMINE; CYSTEAMINE; DISULFIDES; DNA, BACTERIAL; ENDOTOXINS; ESCHERICHIA COLI; FEMALE; HAPLORHINI; HUMANS; INCLUSION BODIES; LYMPHOCYTE ACTIVATION; OXIDATION-REDUCTION; PROTEIN DENATURATION; PROTEIN FOLDING; RATS; RATS, WISTAR; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; SEQUENCE ANALYSIS, PROTEIN; SULFITES; TETRATHIONIC ACID;

EID: 0033999554     PISSN: 0264410X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0264-410X(99)00482-X     Document Type: Article

References (24)

1. Talwar G.P., Sharma N.C., Dubey S.K., Salahuddin M., Das C., Ramakrishna S., Kumar S., Hingorani V. Isoimmunization against human chorionic gonadotropin with conjugates of processed β-subunit of the hormone and tetanus toxoid. Proc. Natl. Acad. Sci. USA. 73:1976;213-222.
2. Thau R.B., Sundaram K., Thorton Y.S., Seidman L.S. Effects of immunization with β-subunit of ovine luteinizing hormone on corpus luteum functions in the rhesus monkey. Fert. Steril. 31:1979;200-204.
3. Singh O., Rao L.V., Gaur A., Sharma N.C., Alam A., Talwar G.P. Antibody response and characteristics of antibodies in women immunized with three contraceptive vaccines inducing antibodies against human chorionic gonadotropin. Fert. Steril. 52:1989;739-744.
4. Jones W.R., Judd S.J., Ing R.M.Y., Griffin P.D., Stevens V.C. Phase-I clinical trials on a World Health Organization birth control vaccine. Lancet. 1:1988;1295-1298.
5. Talwar G.P., Singh O., Pal R., Chatterjee N., Sahai P., Dhall K., Kaur J., Das S.K., Suri S., Buckshee K., Saraya L., Saxena B.N. A vaccine that prevents pregnancy in women. Proc. Natl. Acad. Sci. USA. 91:1994;8532-8536.
6. Stevens V.C. Progress in the development of human chrionic gonadotropin antifertility vaccines. Am. J. Reprod. Immunol. 35:1996;148-155.
7. Reddy V.B., Becy A.K., Garramore A.J., Velluci V., Lustbadar L., Bernstine E.G. Expression of human chorionic gonadotropin in monkey cells using a single simian virus 40 vector. Proc. Natl. Acad. Sci. USA. 8:1985;3644-3648.
8. Lustbader J., Birken S., Pollock S., Levinson L., Berstein E., Hoiung N., Canfield R. Characterization of the expression of products of recombinant human chorionic gonadotropin and subunits. J. Bio. Chem. 262:1987;14204-14212.
9. Chen W., Shen Q.W., Bahl O.P. Carbohydrate varient of the recombinant β-subunit of human chorionic gonadotropin expressed in baculovirus expression system. J. Biol. Chem. 266:1991;4082-4087.
10. Srinivasan J., Singh O., Paul R., Lall L., Chakrobarty S., Talwar G.P. Griffine P.D., Johnson P.M. Local immunity in reproductive tract tissues. 1993;477-481 Oxford University Press, New Delhi.
11. Huth J.R., Norton S.E., Lockridge O., Shikone T., Hsueh A.J.W., Ruddon R.W. Bacterial expression and in vitro folding of the β-subunit of human gonadotropin (hCGβ) and functional assembly of recombinant hCGβ with hCGα Endocrinol. 135:1994;911-918.
12. Mukhopadhyay A., Bhatia P.K., Majumdar S.S. Preliminary studies with recombinant chorionic gonadotropin β-subunit produced in Escherichia coli for use as an antigen in a birth control vaccine. Am. J. Reprod. Immunol. 39:1998;172-182.
13. Studier F.W., Moffatt B.A. J. Mol. Biol. 189:1986;113-130.
14. Panda A., Gorpade A., Mukhopadhyay A., Talwar G.P., Garg L. High cell density fermentation of recombinant Vibrio cholerae for the production of B-subunit of E. coli enterotoxin. Biotechnol. Bioeng. 45:1995;245-250.
15. Mukhopadhyay A. Inclusion bodies and purification of proteins in biologically active forms. Scheper T. Adv. Biochem. Engg./Biotechnol. 56:1997;61-110 Springer, Berlin.
16. Khandjian E.W. Optimized hybridization of DNA blotted and fixed to nitrocellulose and nylon membrane. Bio/Technol. 5:1987;165-167.
17. Maggio E.T. Enzyme Immunoassay. 1981;CRC Press, Boca Raton, Fla.
18. Dighe R.R., Moudgal N.R. Use of α- and β-specific antibodies in studying interaction of hCG with Leydig cell receptors. Arch. Biochem. Biophys. 225:1983;490-499.
19. Shastri N., Dubey S.K., Ragavan V., Salahuddin M., Talwar G.P. Differential affinity of auto Pr-βhCG-TT antibodies for hCG and hLH. Contraception. 18:1978;23-28.
20. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
21. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
22. Lapthorn A.J., Harris D.C., Littlejohn A., Lustbader J.W., Canfield R.E., Machin K.J., Morgan F.J., Issaacs N.W. Nature. 369:1994;455-461.
23. Ben-Bassat A. Method for removing N-terminal methionine from recombinant protein. Seethuram R., Sharma S.K. Purification and analysis of recombinant proteins. 1991;147 Marcel Dekkar Inc, New York.
24. Dirnhofer S., Madersbacher S., Bidart J.M., Ten-kortenaar P.B., Spottl G., Mann K., Wick G., Berger P. The molecular basis for epitopes on the free β-subunit of human chorionic gonadotropin, its carboxyl-terminal peptide and the hCGβ-core fragment. J. Endocrinol. 141:1994;153-162.