Purification and characterization of cationic chymotrypsin from the pancreas of the Arabian camel (Camelus dromedarius)

Molecular and Cellular Biochemistry

Volumn 203, Issue 1-2, 2000, Pages 73-78

  Al Ajlan, A ^a   Bailey, G S ^a  

^a UNIVERSITY OF ESSEX   (United Kingdom)

Author keywords

Camel cationic chymotrypsin; Characterization; Purification

Indexed keywords

APROTININ; AROMATIC AMINO ACID; CALCIUM CHLORIDE; CATION; CHYMOTRYPSIN; CHYMOTRYPSIN A; KININASE; TRYPSIN INHIBITOR;

AFFINITY CHROMATOGRAPHY; ANIMAL TISSUE; ANION EXCHANGE; ARTICLE; CATION EXCHANGE; ENZYME ACTIVITY; ENZYME PURIFICATION; GEL ELECTROPHORESIS; NONHUMAN; PANCREAS; PH;

EID: 0033999419     PISSN: 03008177     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1007097231327     Document Type: Article

References (22)

1. Yargil R: The Desert Camel. Karger, Basel, 1985, 186 pp
2. Bricteux-Gregoire S, Schyns R, Florkin M: Phylogeny of trypsinogen activation peptides. Comp Biochem Physiol 42B: 23-39, 1972
3. Welling GW, Groen G, Beintema JJ: The amino acid sequence of dromedary pancreatic ribonuclease. Biochem J 147: 505-511, 1975
4. Rafiq A, Bailey GS: Purification and characterization of cationic trypsin from the pancreas of the Arabian camel (Cornelius dromedarius). Comp Biochem Physiol 115B: 363-367, 1996
5. Al-Ajlan A, Bailey GS: Purification and partial characterization of camel anionic chymotrypsin. Arch Biochem Biophys 348: 363-368, 1997
6. Al-Ajlan A, Bailey GS: Purification and some properties of camel carboxypeptidase B. Mol Cell Biochem (in press)
7. Gratecos D, Guy O, Rovery M, Desnuelle P: On the two anionic chymotrypsinogens of porcine pancreas. Biochim Biophys Acta 175: 82-96, 1969
8. Wilcox PE: Chymotrysinogens-chymotrypsins. Meth Enzymol 19: 64-108, 1970
9. Folk JE, Schirmer WE: Chymotrypsin C. I. Isolation of the zymogen and the active enzyme: preliminary structure and specificity studies. J Biol Chem 240: 181-192, 1965
10. Keil-Dlouha V, Puigserver A Marie A, Keil B: On subunit II of bovine procarboxypeptidase A. Enzymatic specificity of the tryptic activation. Biochim Biophys Acta 276: 531-535, 1972
11. Peanasky RJ, Gratecos D, Baratti J, Rovery M: Mode of activation and N terminal sequence of subunit II in bovine procarboxypeptidase A and of porcine chymotrypsinogen C. Biochim Biophys Acta 181: 82-92, 1969
12. Rafiq A, Bailey G: A more sensitive Hummel assay for chymotrypsin. Anal Biochem 257: 233-234, 1998
13. Bieth J, Spiess B, Wermuth CG: The synthesis and analytical use of a highly sensitive and convenient substrate of elastase. Biochem Med 11:350-357, 1974
14. Walsh KA, Wilcox PE: Serine proteases. Meth Enzymol 19: 31-41, 1970
15. Folk JE, Schirmer EW: The porcine pancreatic carboxypeptidase A system. J Biol Chem 238: 3884-3894, 1963
16. Folk JE, Piez KA, Carroll WR, Gladner J: Carboxypeptidase B. IV. Purification and characterization of the porcine enzyme. J Biol Chem 235:2272-2277, 1960
17. Bailey GS, Al-Joufi A, Rawat S, Smith DC: Neutralization of kinin-releasing enzymes from crotalid snakes by monospecific and poly-specific antivenoms. Toxicon 29: 777-781, 1991
18. Eisenthal R, Cornish-Bowden A: The direct linear plot. Biochem J 139:715-720, 1974
19. i. Biochem J 129: 197-202, 1972
20. Stoscheck CMM: Quantitation of protein. Meth Enzymol 82: 50-68, 1990
21. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685, 1970
22. Sampaio CAM, Nunes ST, Gracia MD, Mazzacoratti M, Prado JL: Inactivation of kinins by chymotrypsin. Biochem Pharm 25: 2391-2394, 1976