Quantitative analysis of cytomegalovirus load using a real-time PCR assay

Journal of Medical Virology

Volumn 60, Issue 4, 2000, Pages 455-462

  Tanaka, Naoko ^a   Kimura, Hiroshi ^a   Iida, Keiji ^b   Saito, Yumiko ^b   Tsuge, Ikuya ^a   Yoshimi, Ayami ^c   Matsuyama, Takaharu ^c   Morishima, Tsuneo ^a  

^a NAGOYA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b SRL INC   (Japan)

^c JAPANESE RED CROSS NAGOYA FIRST HOSPITAL   (Japan)

Author keywords

CMV; pp65 antigenemia assay; Quantitative PCR; TaqMan

Indexed keywords

VIRUS DNA;

ADOLESCENT; ADULT; ARTICLE; CHILD; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; FEMALE; HUMAN; IMMUNE STATUS; LEUKOCYTE; MAJOR CLINICAL STUDY; MALE; PLASMA; POLYMERASE CHAIN REACTION; QUANTITATIVE ASSAY; VIRUS DETECTION; VIRUS GENOME; VIRUS LOAD; VIRUS TITRATION;

ADOLESCENT; ADULT; ANTIGENS, VIRAL; CHILD; CHILD, PRESCHOOL; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA, VIRAL; FEMALE; HUMANS; IMMEDIATE-EARLY PROTEINS; INFANT; MALE; PHOSPHOPROTEINS; POLYMERASE CHAIN REACTION; VIRAL LOAD; VIRAL MATRIX PROTEINS;

CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS; DNA VIRUSES;

EID: 0033997572     PISSN: 01466615     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1096-9071(200004)60:4<455::AID-JMV14>3.0.CO;2-Q     Document Type: Article

References (32)

1. Akrigg A, Wilkinson GW, Oram JD. 1985. The structure of the major immediate early gene of human cytomegalovirus strain AD169. Virus Res 2:107-121.
2. Ando Y, Kimura H, Miwata H, Kudo T, Shibata M, Morishima T. 1993. Quantitative analysis of herpes simplex virus DNA in cerebrospinal fluid of children with herpes simplex encephalitis. J Med Virol 41:170-173.
3. Boeckh M, Gallez-Hawkins GM, Myerson D, Zaia JA, Bowden RA. 1997. Plasma polymerase chain reaction for cytomegalovirus DNA after allogeneic marrow transplantation: comparison with polymerase chain reaction using peripheral blood leukocytes, pp65 antigenemia, and viral culture. Transplantation 64:108-113.
4. Boeckh M, Boivin G. 1998. Quantitation of cytomegalovirus: methodological aspects and clinical applications. Clin Microbiol Rev 11: 533-554.
5. Boivin G, Handfield J, Murray G, Toma E, Lalonde R, Lazar JG, Bergeron MG. 1997. Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System. J Clin Microbiol 35:525-526.
6. Bossart W, Bienz K, Wunderli W. 1997. Surveillance of cytomegalovirus after solid-organ transplantation: comparison of pp65 antigenemia assay with a quantitative DNA hybridization assay. J Clin Microbiol 35:3303-3304.
7. Cope AV, Sweny P, Sabin C, Rees L, Griffiths PD, Emery VC. 1997. Quantity of cytomegalovirus viruria is a major risk factor for cytomegalovirus disease after renal transplantation. J Med Virol 52:200-205.
8. Gerna G, Revello MG, Percivalle E, Zavattoni M, Parea M, Battaglia M. 1990. Quantification of human cytomegalovirus viremia by using monoclonal antibodies to different viral proteins. J Clin Microbiol 28:2681-2688.
9. Gerna G, Zipeto D, Parea M, Revello MG, Silini E, Percivalle E, Zavattoni M, Grossi P, Milanesi G. 1991. Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia, and DNAemia. J Infect Dis 164:488-498.
10. Gerna G, Zavattoni M, Baldanti F, Sarasini A, Chezzi L, Grossi P, Revello MG. 1998. Human cytomegalovirus (HCMV) leukanemia correlates more closely with clinical symptoms than antigenemia and viremia in heart and heart-lung transplant recipients with primary HCMV infection. Transplantation 65:1378-1385.
11. Gleaves CA, Smith TF, Shuster EA, Pearson GR. 1985. Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens. J Clin Microbiol 21: 217-221.
12. Grossi P, Minoli L, Percivalle E, Irish W, Vigano M, Gerna G. 1995. Clinical and virological monitoring of human cytomegalovirus infection in 294 heart transplant recipients. Transplantation 59: 847-851.
13. Grundy JE, Lawson KM, MacCormac LP, Fletcher JM, Yong KL. 1998. Cytomegalovirus-infected endothelial cells recruit neutrophils by the secretion of C-X-C chemokines and transmit virus by direct neutrophil-endothelial cell contact and during neutrophil transendothelial migration. J Infect Dis 177:1465-1474.
14. Hebart H, Kanz L, Jahn G, Einsele H. 1998. Management of cytomegalovirus infection after solid-organ or stem-cell transplantation. Current guidelines and future prospects. Drugs 55:59-72.
15. Heid CA, Stevens J, Livak KJ, Williams PM. 1996. Real time quantitative PCR. Genome Res 6:986-994.
16. Kennedy MM, Cooper K, Howells DD, Picton S, Biddolph S, Lucas SB, McGee JO, O'Leary JJ. 1998. Identification of HHV8 in early Kaposi's sarcoma: implications for Kaposi's sarcoma pathogenesis. Mol Pathol 51:14-20.
17. Kimura H, Wang Y, Pesnicak L, Cohen JI, Hooks JJ, Straus SE, Williams RK. 1998. Recombinant varicella-zoster virus glycoproteins E and I: immunologic responses and clearance of virus in a guinea pig model of chronic uveitis. J Infect Dis 178:310-317.
18. Kimura H, Morita M, Yabuta Y, Kuzushima K, Kato K, Kojima S, Matsuyama T, Morishima T. 1999. Quantitative analysis of Epstein-Barr virus load by using a real-time PCR assay. J Clin Microbiol 37:132-136.
19. Lazzarotto T, Guerra B, Spezzacatene P, Varani S, Gabrielli L, Pradelli P, Ruinpianesi F, Banzi C, Bovicelli L, Landini MP. 1998. Prenatal diagnosis of congenital cytomegalovirus infection. J Clin Microbiol 36:3540-3544.
20. Limaye AP, Bowden RA, Myerson D, Boeckh M. 1997. Cytomegalovirus disease occurring before engraftment in marrow transplant recipients. Clin Infect Dis 24:830-835.
21. Mocarski ES. 1996. Cytomegalovirus and their replication. In: Fields BN, Knipe DM, Howley PM, editors. Fields virology. Philadelphia: Lippincott-Raven Publishers. p 2447-2492.
22. Morris T, Robertson B, Gallagher M. 1996. Rapid reverse transcription-PCR detection of hepatitis C virus RNA in serum by using the TaqMan fluorogenic detection system. J Clin Microbiol 34:2933-2936.
23. Negishi H, Yamada H, Hirayama E, Okuyama K, Sagawa T, Matsumoto Y, Fujimoto S. 1998. Intraperitoneal administration of cytomegalovirus hyperimmunoglobulin to the cytomegalovirus-infected fetus. J Perinatol 18:466-469.
24. Numazaki K, Chiba S. 1997. Current aspects of diagnosis and treatment of cytomegalovirus infections in infants. Clin Diagn Virol 8:169-181.
25. Shibata M, Terashima M, Kimura H, Kuzushima K, Yoshida J, Horibe K, Morishima T. 1992. Quantitation of cytomegalovirus DNA in lung tissue of bone marrow transplant recipients. Hum Pathol 23:911-915.
26. Shinkai M, Spector SA. 1995. Quantitation of human cytomegalovirus (HCMV) DNA in cerebrospinal fluid by competitive PCR in AIDS patients with different HCMV central nervous system diseases. Scand J Infect Dis 27:559-561.
27. Spector SA, Merrill R, Wolf D, Dankner WM. 1992. Detection of human cytomegalovirus in plasma of AIDS patients during acute visceral disease by DNA amplification. J Clin Microbiol 30:2359-2365.
28. Stagno S. 1995. Cytomegalovirus. In: Remington JS, Klein JO, editors. Infectious diseases of the fetus and newborn infant. Philadelphia: WB Saunders Co. p 312-353.
29. van der Meer JT, Drew WL, Bowden RA, Galasso GJ, Griffiths PD, Jabs DA, Katlama C, Spector SA, Whitley RJ. 1996. Summary of the International Consensus Symposium on advances in the diagnosis, treatment and prophylaxis and cytomegalovirus infection. Antiviral Res 32:119-140.
30. Veal N, Payan C, Fray D, Sarol L, Blanchet O, Kouyoumdjian S, Lunel F. 1996. Novel DNA assay for cytomegalovirus detection: comparison with conventional culture and pp65 antigenemia assay. J Clin Microbiol 34:3097-3100.
31. Whitley RJ, Jacobson MA, Friedberg DN, Holland GN, Jabs DA, Dieterich DT, Hardy WD, Polis MA, Deutsch TA, Feinberg J, Spector SA, Walmsley S, Drew WL, Powderly WG, Griffiths PD, Benson CA, Kessler HA. 1998. Guidelines for the treatment of cytomegalovirus diseases in patients with AIDS in the era of potent antiretroviral therapy: recommendations of an international panel. International AIDS Society-USA. Arch Intern Med 158:957-969.
32. Yamamoto M, Kimura H, Hironaka T, Hirai K, Hasegawa S, Kuzushima K, Shibata M, Morishima T. 1995. Detection and quantification of virus DNA in plasma of patients with Epstein-Barr virus-associated diseases. J Clin Microbiol 33:1765-1768.