Influence of preservatives on conjunctival cells in vitro

Journal of Toxicology - Cutaneous and Ocular Toxicology

Volumn 19, Issue 1, 2000, Pages 79-88

  Debbasch, Caroline ^a   De Saint Jean, Magda ^b   Pisella, Pierre Jean ^b   Rat, Patrice ^a   Warnet, Jean Michel ^a   Baudouin, Christophe ^b  

^a CENTRE HOSPITALIER NATIONAL D OPHTALMOLOGIE DES QUINZE VINGTS   (France)

^b HÔPITAL AMBROISE PARÉ   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BENZALKONIUM CHLORIDE; PRESERVATIVE;

APOPTOSIS; ARTICLE; CELL VIABILITY; CHROMATIN CONDENSATION; CONJUNCTIVA; CONTROLLED STUDY; CYTOTOXICITY; EYE INJURY; EYE TOXICITY; HUMAN; HUMAN CELL;

EID: 0033995279     PISSN: 07313829     EISSN: None     Source Type: Journal    
DOI: 10.3109/15569520009051480     Document Type: Article

References (18)

1. W. N. Wilson, A. J. Duncan, and J. L. Jay, Effects of benzalkonium chloride on the stability of the precorneal tear film in rabbit and man, Br. J. Ophthalmol. 105: 670-673, 1988.
2. M. S. Norn, and A. Opauszki, Effects of ophthalmic vehicles on the stability of the precorneal film, Acta Ophthalmol. 55: 23-34, 1977.
3. C. Baudouin, Side effects of antiglaucomatous drugs on the ocular surface, Curr. Opin. Ophthalmol. 7: 80-86, 1996.
4. F. Becquet, M. Goldschild, M. S. Moldovan, M. Ettaiche, P. Gastaud, and C. Baudouin, Histopathological effects of topical ophthalmic preservatives on rat corneoconjunctival surface, Curr. Eye Res. 17: 419-425, 1998.
5. P. Rat, M. O. Christen, M. Thevenin, J.-M. Warnet, and M. Adolphe, Cold light fluorimetry: A microtitration technology for cell culture to evaluate anethole dithiolethione and other biothiols, Methods Enzymol. 252: 331-341, 1995.
6. M. Balls and J.-H. Fentem, The use of basal cytotoxicity and target organ toxicity tests in hazard identification and risk assessment, ATLA 20: 368-388, 1992.
7. E. Borenfreund and J. A. Puerner, A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/NR-90), J. Tissue Cult. Methods, 9: 7-9, 1984.
8. P. Rat, C. Korwin-Zmilowska, J. M. Warnet, and M. Adolphe, New in vitro fluorimetric microtitration assays for toxicological screening of drugs, Cell Biol. Toxicol. 10: 329-337, 1994.
9. Z. Darzynkiewicz, G. Juan, X. Li, W. Gorczyca, T. Murakami, and F. Traganos, Cytometry in cell necrobiology: Analysis of apoptosis and accidental cell death (necrosis), Cytometry 27: 1-20, 1997.
10. P. Rat, R. Osseni, S. Toure, M. O. Christen, M. Thevenin, J. M. Warnet, M. Adolphe, Microtitration fluorimetric assays on alive cells (MiFAAC tests) used for tacrine cytotoxicity evaluation. International Congress on Hepatocytes. (Tübingen, Germany, Sept. 1996), Cell Biol. Toxicol. S1: 38, 1996.
11. Z. Darzynkiewicz, S. Bruno, G. Del Bino, W. Gorczyca, M. A. Hotz., P. Lassota, and F. Trigands, Features of apoptotic cells measured by flow cytometry, Cytometry 13: 795-808, 1992.
12. Y. Hirabayashi, S. Tanuichi, and M. S. Kaboyashi, A quantitative assay of oxidative metabolism by neutrophils in whole blood using flow cytometry, J. Immunol. Methods 82: 253-259, 1985.
13. G. C. Rothe, and G. Valet, Flow cytometric assays of oxidative burst activity in phagocytes, Methods Enzymol. 233: 539-548, 1990.
14. D. Han, C. K. Sen, S. Roy, M. S. Kobayashi, H. J. Tritschler, and L. Packer, Protection against glutamate-induced cytotoxicity in C6 glial cells by thiol antioxidants, Am. J. Physiol. 273: 177-178, 1997.
15. S. Tan, Y. Sagara, Y. Liu, P. Maher, and D. Schubert, The regulation of oxygen species production during programmed cell death, J. Cell. Biol. 141: 1423-1432, 1998.
16. C. Baudouin, P. J. Pisella, K. Fillacier, M. Goldschild, F. Becquet, M. De Saint Jean, and A. Bechetoille, Ocular surface inflammatory changes induced by topical antiglaucoma drugs. Human and animal studies, Ophthalmology 106: 556-563, 1999.
17. N. L. Burstein, The effects of topical drugs and preservatives on the tears and corneal epithelium in dry eye, Trans. Ophthalmol. Soc. UK 104: 402-409, 1985.
18. F. Belloc, P. Dumain, M. R. Boisseau, C. Jalloustre, J. Reiffers, P. Bernard, and F. Lacombe, A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle and apoptosis determination in unfixed cells, Cytometry 17: 59-65, 1994.