Digital analysis of light microscope immunofluorescence: High-resolution co-localization of synaptic proteins in cultured neurons

Journal of Neuroscience Methods

Volumn 96, Issue 1, 2000, Pages 1-9

  Hutcheon, B ^a   Brown, L A ^a   Poulter, M O ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Cortex; Deconvolution; GABAA receptor; Imaging; Synapse

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; MONOCLONAL ANTIBODY; RECEPTOR PROTEIN; RECEPTOR SUBUNIT; SYNAPTOPHYSIN;

ANIMAL CELL; ARTICLE; BRAIN CORTEX; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; EMBRYO; IMAGE RECONSTRUCTION; NERVE CELL CULTURE; NEUROTRANSMISSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT; SYNAPSE;

ANIMALS; ANTIBODIES; CEREBRAL CORTEX; FLUORESCENT ANTIBODY TECHNIQUE; IMAGE PROCESSING, COMPUTER-ASSISTED; MICROSCOPY, FLUORESCENCE; MICROSPHERES; NEURONS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, GABA-A; SYNAPSES;

EID: 0033995111     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(99)00148-X     Document Type: Article

References (8)

1. Baude A., Nusser Z., Molnar E., McIlhinney R.A., Somogyi P. High-resolution immunogold localization of AMPA type glutamate receptor subunits at synaptic and non-synaptic sites in rat hippocampus. Neuroscience. 69:1995;1031-1055.
2. De Felipe J., Marco P., Fairen A., Jones E.G. Inhibitory synaptogenesis in mouse somatosensory cortex. Cereb. Cortex. 7:1997;619-634.
3. Koch C., Segev I. Methods in neuronal modeling: from ions to networks. 2nd ed:1998;MIT Press, Cambridge, MA. 671 pp.
4. Mailly P., Gastard M., Cupo A. Subcellular distribution of δ-opioid receptors in the rat spinal cord: an approach using the three-dimensional reconstruction of confocal series of immunolabelled neurons. J. Neurosci. Methods. 87:1999;17-24.
5. McNally J.G., Preza C., Conchello J.A., Thomas L.J. Artifacts in computational optical-sectioning microscopy. J. Opt. Soc. Am. A. 11:1994;1056-1067.
6. A receptor in relation to specific GABAergic synapses in the dentate gyrus. Eur. J. Neurosci. 7:1995;630-646.
7. Shaw P. Comparison of wide-field/deconvolution and confocal microscopy for 3D imaging. Pawley J.B. Handbook of biological and confocal microscopy. 1995;373-387 Plenum, New York.
8. Tamas G., Buhl E.H., Somogyi P. Fast IPSPs elicited via multiple synaptic release sites by different types of GABAergic neurone in the cat visual cortex. J. Physiol. (Lond.). 500:1997;715-738.