A method for measuring colocalization of presynaptic markers with anatomically labeled axons using double label immunofluorescence and confocal microscopy

Journal of Neuroscience Methods

Volumn 94, Issue 2, 2000, Pages 205-215

  Silver, Michael A ^a   Stryker, Michael P ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Colocalization; Confocal microscope; Immunofluorescence; Lateral geniculate nucleus; Presynaptic terminal; Synaptic vesicle protein; Synaptophysin; Visual cortex

Indexed keywords

MARKER; MEMBRANE PROTEIN; SYNAPTOPHYSIN;

ANIMAL CELL; ANIMAL TISSUE; ANTIGEN EXPRESSION; ARTICLE; CAT; CONFOCAL MICROSCOPY; CONTROLLED STUDY; IMMUNOFLUORESCENCE; NERVE CELL PLASTICITY; NERVE ENDING; NERVE FIBER; NEUROTRANSMISSION; NONHUMAN; PRESYNAPTIC NERVE; PRIORITY JOURNAL; PROTEIN LOCALIZATION; STAINING; VISUAL CORTEX;

ANIMALS; ARTIFACTS; AXONS; BIOLOGICAL MARKERS; CATS; DIFFERENTIAL THRESHOLD; FLUORESCENT ANTIBODY TECHNIQUE; MICROSCOPY, CONFOCAL; NEUROTRANSMITTER AGENTS; PHYTOHEMAGGLUTININS; PRESYNAPTIC TERMINALS; SYNAPTOPHYSIN; TISSUE DISTRIBUTION;

EID: 0033991595     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(99)00145-4     Document Type: Article

References (21)

1. Antonini A., Stryker M.P. Development of individual geniculocortical arbors in cat striate cortex and effects of binocular impulse blockade. J Neurosci. 13:1993;3549-3573.
2. Antonini A., Stryker M.P. Rapid remodeling of axonal arbors in the visual cortex. Science. 260:1993;1819-1821.
3. Calhoun M.E., Jucker M., Martin L.J., Thinakaran G., Price D.L., Mouton P.R. Comparative evaluation of synaptophysin-based methods for quantification of synapses. J Neurocytol. 25:1996;821-828.
4. Carrington W.A., Fogarty K.E., Lifschitz L., Fay F.S. Three-dimensional imaging on confocal and wide-field microscopes. Pawley J.B. Handbook of Biological Confocal Microscopy. revised edition:1990;151-161 Plenum, New York.
5. Chang Y.-C., Gottlieb D.I. Characterization of the proteins purified with monoclonal antibodies to glutamic acid decarboxylase. J Neurosci. 8:1988;2123-2130.
6. Esclapez M., Tillakaratne N.J.K., Kaufman D.L., Tobin A.J., Houser C.R. Comparative localization of two forms of glutamic acid decarboxylase and their mRNAs in rat brain supports the concept of functional differences between the forms. J Neurosci. 14:1994;1834-1855.
7. Freund T.F., Martin K.A.C., Whitteridge D. Innervation of cat visual areas 17 and 18 by physiologically identified X- and Y-type thalamic afferents. I. Arborization patterns and quantitative distribution of postsynaptic elements. J Comp Neurol. 242:1985;263-274.
8. Friedlander M.J., Martin K.A., Wassenhove-McCarthy D. Effects of monocular visual deprivation on geniculocortical innervation of area 18 in cat. J Neurosci. 11:1991;3268-3288.
9. Garey L.J., Powell T.P.S. An experimental study of the termination of the lateral geniculo-cortical pathway in the cat and monkey. Proc R Soc London B. 179:1971;41-63.
10. Hamos J.E., Van Horn S.C., Raczkowski D., Sherman S.M. Synaptic circuits involving an individual retinogeniculate axon in the cat. J Comp Neurol. 259:1987;165-1692.
11. Hooper J.E., Carlson S.S., Kelly R.B. Antibodies to synaptic vesicles purified from Narcine electric organ bind a subclass of mammalian nerve terminals. J Cell Biol. 87:1980;104-113.
12. Humphrey A.L., Sur M., Uhlrick D.J., Sherman S.M. Projection patterns of individual X- and Y-cell axons from the lateral geniculate nucleus to cortical area 17 in the cat. J Comp Neurol. 223:1985;159-189.
13. Kaufman D.L., Houser C.R., Tobin A.J. Two forms of the gamma-aminobutyric acid synthetic enzyme glutatmate decarboxylase have distinct intraneuronal distributions and cofactor interactions. J Neurochem. 56:1991;720-723.
14. LeVay S., Stryker M.P. The development of ocular dominance columns in the cat. Ferrendelli J.A. Society for Neuroscience Symposia: Aspects of Developmental Neurobiology. 4:1979;83-98 Society for Neuroscience, Bethesda, MD.
15. Matthew W.D., Tsavaler L., Reichardt L.F. Identification of a synaptic vesicle-specific membrane protein with a wide distribution in neuronal and neurosecretory tissue. J Cell Biol. 91:1981;257-269.
16. McCarty J.H., Feinstein S.C. Activation loop tyrosines contribute varying roles to TrkB autophosphorylation and signal transduction. Oncogene. 16:1998;1691-1700.
17. Pinches E.M., Cline H.T. Distribution of synaptic vesicle proteins within single retinotectal axons of Xenopus tadpoles. J Neurobiol. 35:1998;426-434.
18. Russ J. The Image Processing Handbook. 2nd edition:1995;CRC Press, Boca Raton, FL.
19. Südhof T.C. The synaptic vesicle cycle: a cascade of protein-protein interactions. Nature. 375:1995;645-653.
20. r 38,000 characteristic of presynaptic vesicles. Cell. 41:1985;1017-1028.
21. Yan Q., Matheson C., Sun J., Radeke M.J., Feinstein S.C., Miller J.A. Distribution of intracerebral ventricularly administered neurotrophins in rat brain and its correlation with Trk receptor expression. Exp Neurol. 127:1994;23-36.