The effect of mutations in EF-Tu on its affinity for tRNA as measured by two novel and independent methods of general applicability

Journal of Biochemical and Biophysical Methods

Volumn 42, Issue 1-2, 2000, Pages 1-14

  Vorstenbosch, Erik L H ^a   Potapov, Anatolij P ^a   De Graaf, J Martien ^a   Kraal, Barend ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

Author keywords

Competition assay; Dissociation rate constant; Equilibrium dissociation constant; His tag; Kirromycin resistance; Protection assay

Indexed keywords

AMINOACYL TRANSFER RNA; ANTIBIOTIC AGENT; ELONGATION FACTOR TU; GUANOSINE TRIPHOSPHATE; HISTIDINE; MOCIMYCIN; MUTANT PROTEIN; PHENYLALANINE TRANSFER RNA; RIBONUCLEASE A; TRANSFER RNA;

ANTIBIOTIC RESISTANCE; ARTICLE; BINDING AFFINITY; BINDING COMPETITION; CONTROLLED STUDY; DISSOCIATION CONSTANT; INTERMETHOD COMPARISON; MUTATION; PRIORITY JOURNAL; PROTECTION; PROTEIN SYNTHESIS; SCREENING; STRUCTURE ACTIVITY RELATION; TECHNIQUE; TEMPERATURE;

BINDING, COMPETITIVE; BIOCHEMISTRY; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; HISTIDINE; MODELS, CHEMICAL; MUTATION; PEPTIDE ELONGATION FACTOR TU; PEPTIDES; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; RIBONUCLEASE, PANCREATIC; RNA, TRANSFER, AMINO ACYL; RNA, TRANSFER, PHE;

EID: 0033990904     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(99)00032-9     Document Type: Article

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