Differences in substrate specificity and kinetic properties of the recombinant hexokinases HXK1 and HXK2 from Entamoeba histolytica

Molecular and Biochemical Parasitology

Volumn 105, Issue 1, 2000, Pages 71-80

  Kroschewski, Helga ^a   Ortner, Stephan ^a   Steipe, Boris ^b   Scheiner, Otto ^a   Wiedermann, Gerhard ^a   Duchêne, Michael ^a  

^a UNIVERSITY OF VIENNA   (Austria)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

Entamoeba histolytica; Enzyme kinetics; Enzyme substrates; Fructose; Glucose; Hexokinase; Mannose

Indexed keywords

FRUCTOKINASE; FRUCTOSE; GALACTOSE; GLUCOKINASE; HEXOKINASE;

AMINO ACID SUBSTITUTION; ARTICLE; COLITIS; ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME KINETICS; ENZYME PHOSPHORYLATION; ENZYME SPECIFICITY; LIVER ABSCESS; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENTAMOEBA HISTOLYTICA; HEXOKINASE; KINETICS; MODELS, MOLECULAR; MUTATION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

ENTAMOEBA DISPAR; ENTAMOEBA HISTOLYTICA; PROTOZOA;

EID: 0033990619     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(99)00164-4     Document Type: Article

References (23)

1. Walsh J.A. Problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and mortality. Rev Infect Dis. 8:1986;228-238.
2. Clark C.G., Roger A.J. Direct evidence for secondary loss of mitochondria in Entamoeba histolytica. Proc Natl Acad Sci USA. 92:1995;6518-6521.
3. Reeves R.E. Metabolism of Entamoeba histolytica Schaudinn, 1903. Adv Parasitol. 23:1984;105-142.
4. Reeves R.E., Montalvo F., Sillero A. Glucokinase from Entamoeba histolytica and related organisms. Biochemistry. 6:1967;1752-1760.
5. Bracha R., Chayen A., Rosenberg I., Warren L.G., Mirelman D. Isolation and partial characterisation of the hexokinase isoenzymes from pathogenic and non-pathogenic strains of Entamoeba histolytica. Mol Biochem Parsitol. 25:1987;203-212.
6. Ortner S., Plaimauer B., Binder M., Scheiner O., Wiedermann G., Duchêne M. Molecular analysis of two hexokinase isoenzymes from Entamoeba histolytica. Mol Biochem Parasitol. 73:1995;189-198.
7. Diamond L.S., Clark C.G. A redescription of Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911) separating it from Entamoeba dispar Brumpt, 1925. J Euk Microbiol. 40:1993;340-344.
8. Farri T.A., Sargeaunt P.G., Warhurst D.C., Williams J.E., Bhojnani R. Electrophoretic studies of the hexokinase of Entamoeba histolytica groups I to IV. Trans R Soc Trop Med Hyg. 74:1980;672-673.
9. Ortner S., Clark C.G., Binder M., Scheiner O., Wiedermann G., Duchêne M. Molecular biology of the hexokinase isoenzyme pattern that distinguishes pathogenic Entamoeba histolytica from nonpathogenic Entamoeba dispar. Mol Biochem Parasitol. 86:1997;85-94.
10. Wilson J.E. Hexokinases. Rev Physiol Biochem Pharmacol. 126:1995;65-198.
11. Cárdenas M.L., Cornish-Bowden A., Ureta T. Evolution and regulatory role of the hexokinases. 1401:1998;242-264.
12. Kunkel T.A., Roberts J.D., Zakour R.A. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154:1987;367-382.
13. Tabor S., Richardson C.C. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA. 82:1985;1074-1078.
14. α in Escherichia coli. Purification and properties of two forms of the protein. J Biol Chem. 264:1989;409-418.
15. Schupp J.M., Travis S.E., Price L.B., Shand R.F., Keim P. Rapid bacterial permeabilization reagent useful for enzyme assays. BioTechniques. 19:1995;18-20.
16. Froehlich K.U., Entian K.D., Mecke D. The primary structure of the yeast hexokinase PII gene (HXK2) which is responsible for glucose repression. Gene. 36:1985;105-111.
17. Anderson C.M., Stenkamp R.E., Steitz T.A. Sequencing a protein by X-ray crystallography. II. Refinement of yeast hexokinase B co-ordinates and sequence at 2.1 Å resolution. J Mol Biol. 123:1978;15-33.
18. Tielens A.G.M., van den Heuvel J.M., van Mazijk H.J., Wilson J.E., Shoemaker C.B. The 50-kDa glucose 6-phosphate sensitive hexokinase of Schistosoma mansoni. J Biol Chem. 269:1994;24736-24741.
19. Barnard E.A. Hexokinases from yeast. Methods Enzymol. 42:1975;6-20.
20. Villagómez-Castro J.C., Calvo-Méndez C., Vargas-Rodrígues L., Flores-Carreón A., López-Romero E. Entamoeba histolytica: solubilization and biochemical characterization of dolichol phosphate mannose synthase, an essential enzyme in glycoprotein biosynthesis. Exp Parasitol. 88:1998;111-120.
21. Marinets A., Zhang T., Guillén N., Gounon P., Bohle B., Vollmann U., Scheiner O., Wiedermann G., Stanley S.L., Duchêne M. Protection against invasive amebiasis by a single monoclonal antibody directed against a lipophosphoglycan antigen localized on the surface of Entamoeba histolytica. J Exp Med. 186:1997;1557-1565.
22. Weinbach E.C., Diamond L.S. Entamoeba histolytica: I. Aerobic metabolism. Exp Parasitol. 35:1974;232-243.
23. Aleshin A.E., Zeng C., Bourenkov G.P., Bartunik H.D., Fromm H.J., Honzatko R.B. The mechanism of regulation of hexokinase: new insights from the crystal structure of recombinant human brain hexokinase complexed with glucose and glucose-6-phosphate. Structure. 6:1998;39-50.