An automated biological assay to determine levels of the trypanocidal drug melarsoprol in biological fluids

Acta Tropica

Volumn 74, Issue 1, 2000, Pages 95-100

  Onyango, J D ^a   Burri, C ^b   Brun, R ^b  

^a Kenya Trypanosomiasis Research Institute   (Kenya)

^b SWISS TROPICAL AND PUBLIC HEALTH INSTITUTE   (Switzerland)

Author keywords

Alamar blue; Bioassay; Fluorometry; Melarsoprol; Trypanosoma brucei

Indexed keywords

ALAMAR BLUE; FLUORESCENT DYE; MELARSOPROL; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; AUTOMATION; BIOASSAY; CONTROLLED STUDY; DRUG BLOOD LEVEL; DRUG CEREBROSPINAL FLUID LEVEL; DRUG DETERMINATION; FLUORESCENCE; HUMAN; HUMAN TISSUE; PARASITE CULTIVATION; QUANTITATIVE ASSAY; TECHNIQUE; TRYPANOSOMA RHODESIENSE;

ANIMALS; BIOLOGICAL ASSAY; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COLORING AGENTS; DRUG MONITORING; FLUOROMETRY; HUMANS; MALE; MELARSOPROL; OXAZINES; REPRODUCIBILITY OF RESULTS; TRYPANOCIDAL AGENTS; TRYPANOSOMA BRUCEI BRUCEI; XANTHENES;

TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0033990450     PISSN: 0001706X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0001-706X(99)00080-7     Document Type: Article

References (21)

1. Baltz T., Baltz D., Giroud C., Crockett J. Cultivation in a semi-defined medium of animal infective forms of Trypanosoma brucei, T. equiperdum, T. evansi, T. rhodesiense and T. gambiense. EMBO J. 4:1985;1273-1277.
2. Berger B.J., Fairlamb A.H. High-performance liquid chromatographic method for the separation and quantitative estimation of anti-parasitic melaminophenyl arsenical compounds. Trans. R. Soc. Trop. Med. Hyg. 88:1994;357-359.
3. Bienen E.J., Webster P., Fish W.R. Trypanosoma (Nannomonas) congolense: changes in respiratory metabolism during life cycle. Exp. Parasitol. 73:1991;403-412.
4. Bronner U., Brun R., Doua F., Ericsson O., Burri C., Keiser J., Miezan T.W., Boa Y.F., Rombo L., Gustafsson L.L. Discrepancy in plasma melarsoprol concentrations between HPLC and bioassay methods in patients with T. gambiense sleeping sickness indicates that melarsoprol is metabolized. Trop. Med. Int. Health. 3:1998;913-917.
5. Brun R., Kunz C. In vitro drug sensitivity test for Trypanosoma brucei subgroup bloodstream trypomastigotes. Acta Trop. 46:1989;361-368.
6. Burri C., Blum J., Brun R. Alternative application of melarsoprol for treatment of T. b. gambiense sleeping sickness. Preliminary results. Ann. Soc. Belge Med. Trop. 75:1995;65-71.
7. Burri C., Brun R. An in vitro bioassay for quantification of melarsoprol in serum and cerebrospinal fluid. Trop. Med. Parasitol. 43:1992;223-225.
8. Burri C., Onyango J.D., Auma J.E., Burudi E.M., Brun R. Pharmacokinetics of melarsoprol in uninfected vervet monkeys. Acta Trop. 58:1994;35-49.
9. Burri C., Giroud C., Doua F., Welker H.A., Brun R. Pharmacokinetic properties of the trypanocidal drug melarsoprol. Chemotherapy. 39:1993;225-234.
10. Ericsson O., Schweda E., Bronner U., Rombo L., Friden M., Gustafsson L.L. Determination of melarsoprol in biological fluids by high-performance liquid chromatography and characterisation of two stereoisomers by nuclear magnetic resonance spectroscopy. J. Chromatogr. B. 690:1997;243-251.
11. Friedheim E.A.H. Mel B in the treatment of human trypanosomiasis. Am. J. Trop. Med. Hyg. 29:1949;173-180.
12. 50 in studies of drug resistance of malaria parasites. Acta Trop. 55:1993;257-261.
13. Kaminsky, R., Chuma, F., Zweygart, E., 1989. Trypanosoma brucei brucei: expression of drug resistance in vitro. Exp. Parasitol. 281-289.
14. Kaminsky R., Zweygarth E. Effect of in vitro cultivation on the stability of resistance of Trypanosoma brucei brucei to diminazene, isometamidium, quinapyramine, and Mel B. J. Parasitol. 75:1989;42-45.
15. Kuzoe F.A.S. Current situation of African trypanosomiasis. Acta Trop. 54:1993;153-162.
16. Obexer W., Schmid C., Brun R. A novel in vitro screening assay for trypanocidal activity using the fluorescent dye BCECF-AM. Trop. Med. Parasitol. 46:1995;45-48.
17. Pepin J., Milord F., Guerin C., Schechter P.J. Difluoromethylornithine for arseno-resistant Trypanosoma brucei gambiense sleeping sickness. Lancet. 81:1987;1431-1433.
18. Räz B., Iten M., Grether-Buhler Y., Kaminsky R., Brun R. The Alamar blue assay to determine drug sensitivity of African trypanosomes (T.b. rhodesiense and T.b. gambiense) in vitro. Acta Trop. 68:1997;139-147.
19. Wellde B.T., Chumo D.A., Reardon M.J., Abinya A., Wanyama L., Dola S., Mbwabi D., Smith D.H., Siongok T.A. Treatment of Rhodesian sleeping sickness in Kenya. Ann. Trop. Med. Parasitol. 83(Suppl. 1):1989;99-109.
20. WHO, 1986. Epidemiology and control of African trypanosomiasis. Technical Report Series no. 739.
21. Zinsstag J., Brun R., Craciunescu D.G., Parrondo Iglesias E. In vitro activity of organometallic complexes of Ir, Pt and Rh on Trypanosoma b. gambiense, T. b. rhodesiense and T. b. brucei. Trop. Med. Parasitol. 42:1991;41-44.