Desensitization characteristics of rat recombinant GABA(A) receptors consisting of α1β2γ(2S) and α1β2 subunits expressed in HEK293 cells

Neuroscience Letters

Volumn 278, Issue 1-2, 2000, Pages 21-24

  Krampfl, Klaus ^a   Bufler, Johannes ^a   Lepier, Alexandra ^b   Dudel, Josef ^b   Adelsberger, Helmuth ^b  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Aminobutyric acid; Desensitization; GABA(A) receptor; HEK293; Patch clamp; Rat; Subunit composition; Whole cell

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; RECEPTOR SUBUNIT;

ARTICLE; DESENSITIZATION; EMBRYO; GABAERGIC SYSTEM; GABAERGIC TRANSMISSION; HUMAN; HUMAN CELL; MEMBRANE CHANNEL; MEMBRANE CURRENT; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT;

ANIMALS; DOSE-RESPONSE RELATIONSHIP, DRUG; GAMMA-AMINOBUTYRIC ACID; KINETICS; PATCH-CLAMP TECHNIQUES; PROTEIN CONFORMATION; RATS; RECEPTORS, GABA-A; RECOMBINANT FUSION PROTEINS;

EID: 0033989858     PISSN: 03043940     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-3940(99)00888-5     Document Type: Article

References (13)

1. Colquhoun D., Sigworth F.J. The Minimum-χ2 Method. Sakmann B., Neher E. Single-Channel Recording. 1995;551-552 Plenum Press, New York.
2. Franke C., Hatt H., Dudel J. Liquid filament switch for ultra-fast exchanges of solutions at excised patches of synaptic membrane of crayfish muscle. Neurosci. Lett. 77:1987;199-204.
3. A receptor subunit gamma2 and delta subtypes confer unique kinetic properties on recombinant GABAA receptor currents in mouse fibroblasts. J. Physiol. 514.1:1999;27-45.
4. Heim R., Cubitt A.B., Tsien R.Y. Improved green fluorescence. Nature. 373:1995;663-664.
5. A receptors. Mol. Neurobiol. 18:1998;35-86.
6. A receptor channels. NeuroReport. 8:1997;3443-3446.
7. A channel responses to brief agonist pulses. Neuron. 15:1995;181-191.
8. Jones M.W., Westbrook G.L. The impact of receptor desensitization on fast synaptic transmission. Trends Neurosci. 19:1996;96-101.
9. A receptor subtypes really occur in the brain? Trends Neurosci. 19:1996;139-143.
10. A receptor determined by electron microscopic image analysis. J. Neurochem. 62:1994;815-818.
11. A receptor subtypes. Pharmacol. Rev. 47:1995;182-234.
12. 2L subunit. Neuron. 7:1991;27-33.
13. Whiting P., McKernan R.M., Iversen L.L. Another mechanism for creating diversity in γ-aminobutyrate type A receptors: RNA splicing directs expression of two forms of γ2 phosphorylation site. PNAS. 87:1990;9966-9970.