Image analysis method to assess adhesion of Helicobacter pylori to gastric epithelium using confocal laser scanning microscopy

Journal of Microbiological Methods

Volumn 39, Issue 3, 2000, Pages 179-187

  Reinhard, Joscha ^a   Basset, Christelle ^c   Holton, John ^b   Binks, Michael ^b   Youinou, Pierre ^c   Vaira, Dino ^d  

^a UNIVERSITY HOSPITAL DÜSSELDORF   (Germany)

^b ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^c BREST UNIVERSITY HOSPITAL   (France)

^d UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

Gastric mucosal cells; Helicobacter pylori; Image analysis

Indexed keywords

FLUORESCEIN ISOTHIOCYANATE; OLIGOSACCHARIDE;

ARTICLE; BACTERIUM ADHERENCE; BLOOD GROUP LEWIS SYSTEM; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; HELICOBACTER PYLORI; HUMAN; HUMAN TISSUE; IMAGE ANALYSIS; INTERNET; NONHUMAN; PRIORITY JOURNAL; STOMACH EPITHELIUM; TECHNIQUE;

BACTERIAL ADHESION; GASTRIC MUCOSA; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LEWIS BLOOD-GROUP SYSTEM; MICROSCOPY, CONFOCAL;

HELICOBACTER PYLORI;

EID: 0033989617     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(99)00118-9     Document Type: Article

References (13)

1. Alkout A.M., Blackwell C.C., Weir D.M., Poxton I.R., Elton R.A., Luman W., Palmer K. Isolation of a cell surface component of H. pylori that binds H Type 2 LewisA and LewisB antigens. Gastroenterology. 112:1997;1179-1187.
2. Angstrom J., Teneberg S., Milh M.A., Larsson T., Leonardsson I., Olsson B.M., Halvarsson J., Danielsson D., Aslund N., Ljungh A., Wadstrom T., Karlsson K.A. The lactosylceramide binding specificity of H. pylori. Glycobiology. 8:1998;297-309.
3. Boren T., Falk P., Roth K.A., Larson G., Normark S. Attachment of H. pylori to human gastric epithelium is mediated by blood group antigens. Science. 262:1993;1892-1895.
4. Chmiela M., Lawnik M., Czkwianianc E., Rechcinski T., Planetmalecka I., Wadstrom T., Rudnicka W. Attachment of Helicobacter pylori strains to human epithelial cells. J. Physiol. Pharmacol. 48:1997;393-404.
5. Clyne M., Thomas J., Weaver L., Drumm B. In vitro evaluation of the role of antibodies against H. pylori in inhibiting adherence of the organism to gastric cells. Gut. 40:1997;731-738.
6. Clyne M., Drumm B. Absence of effect of Lewis A and B expression on adherence of H. pylori to human gastic cells. Gastroenterology. 113:1997;72-80.
7. Schistosomes, liver flukes and Helicobacter pylori. IARC-Monogr.-Eval.-Carcinog.-Risks-Hum. 61:1994;1-241.
8. Ilver D., Arnqvist A., Ogren J., Frick I.M., Kersulyte D., Incecik E.T., Berg D.E., Covacci A., Engstrand L., Boren T. H. pylori adhesin binding fucosylated histo-blood group antigens revealed by retagging. Science. 279:1998;373-377.
9. Lee A., O'Rourke J., De-Ungria M.C., Robertson B., Daskalopoulos G., Dixon M.F. A standardized mouse model of Helicobacter pylori infection: Introducing the Sydney strain. Gastroenterology. 112:1997;1386-1397.
10. Miller-Podraza H., Milh M.A., Bergstrom J., Karlsson K.A. Recognition of glycoconjugates by H. pylori: An apparently high-affinity binding of human polyglycosylceramides, a second sialic acid-based specificity. J. Glycononj. 13:1996;453-460.
11. Moran A.P., Wadstrom T. Pathogenesis of Helicobacter pylori. Current Opinion in Gastroenterology. 14:1998;S9-S14.
12. Helicobacter pylori in peptic ulcer disease. NIH Consensus Development Panel on Helicobacter pylori in Peptic Ulcer Disease. J. Am. Med. Assoc. 272:1994;65-69.
13. Simon P.M., Goode P.L., Mobasseri A., Zopf D. Inhibition of Helicobacter pylori binding to gastrointestinal epithelial cells by sialic acid-containing oligosaccharides. Infect-Immun. 65:1997;750-757.