High-throughput method for isolating plasmid DNA with reduced lipopolysaccharide content

BioTechniques

Volumn 28, Issue 1, 2000, Pages 106-108

  Neudecker, Frank ^a   Grimm, Stefan ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; PLASMID DNA; SILICON DERIVATIVE;

ANALYTIC METHOD; ARTICLE; CONTROLLED STUDY; DNA BINDING; DNA PURIFICATION; EMBRYO; EMBRYO CELL; GENE ISOLATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL;

BACTERIA (MICROORGANISMS);

EID: 0033989420     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00281st06     Document Type: Article

References (7)

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3. Hansen, N. J., P. Kristensen, J. Lykke, K.K. Mortensen and B.F. Clark. 1995. A fast, economical and efficient method for DNA purification by use of a homemade bead column. Biochem. Mol. Biol. Int. 35:461-465.
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5. Vogelstein, B. and D. Gillespie. 1979. Preparative and analytical purification of DNA from agarose. Proc. Natl. Acad. Sci. USA 76:615-619.
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7. Wicks, I.P., M.L. Howell, T. Hancock, H. Kohsaka, T. Olee and D.A. Carson. 1995. Bacterial lipopolysaccharide copurifies with plasmid DNA: implications for animal models and human gene therapy. Hum. Gene Ther. 6:317-323.