Molecular detection of Gluconacetobacter sacchari associated with the pink sugarcane mealybug Saccharicoccus sacchari (Cockerell) and the sugarcane leaf sheath microenvironment by FISH and PCR

FEMS Microbiology Ecology

Volumn 31, Issue 1, 2000, Pages 61-71

  Franke, Ingrid H ^a   Fegan, Mark ^a   Hayward, Chris ^a   Leonard, Graham ^b   Sly, Lindsay I ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b BSES LIMITED   (Australia)

Author keywords

Fluorescence in situ hybridization; Gluconacetobacter sacchari; PCR; Pink sugarcane mealybug

Indexed keywords

BACTERIUM; DETECTION METHOD; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION;

ARTICLE; BACTERIUM DETECTION; EUBACTERIUM; FLUORESCENCE IN SITU HYBRIDIZATION; GENE AMPLIFICATION; GENE SEQUENCE; GLUCONACETOBACTER SACCHARI; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; PLANT LEAF; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHARICOCCUS SACCHARI; TEMPERATURE;

GLUCONACETOBACTER DIAZOTROPHICUS; GLUCONACETOBACTER LIQUEFACIENS; GLUCONACETOBACTER SACCHARI; SACCHARICOCCUS SACCHARI;

EID: 0033988336     PISSN: 01686496     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-6496(99)00082-3     Document Type: Article

References (35)

1. Boddey R.M., Urquiaga S., Reis V., Döbereiner J. Biological nitrogen fixation associated with sugarcane. Plant Soil. 137:1991;111-117.
2. Dong Z., Canny M.J., McCully M.E., Regla Roboredo M., Fernandez Cabadilla C., Ortega E., Rodes R. A nitrogen-fixing endophyte of stems. Plant Physiol. 105:1994;1139-1147.
3. Neyra C.A., Döbereiner J. Nitrogen fixation in grasses. Adv. Agron. 29:1977;1-38.
4. Urquiaga S., Cruz K.H.S., Boddey R.M. Contribution of nitrogen fixation to sugarcane: nitrogen-15 and nitrogen-balance estimates. Soil Sci. Soc. Am. J. 56:1992;105-114.
5. Gillis M., Kersters K., Hoste B., Janssens D., Kroppenstedt M., Stephan M.P., Teixeira K.R.S., Döbereiner J., De Ley J. Acetobacter diazotrophicus sp. nov., a nitrogen-fixing acetic acid bacterium associated with sugarcane. Int. J. Syst. Bacteriol. 39:1989;361-364.
6. Li R., MacRae I.C. Specific association of diazotrophic acetobacters with sugarcane. Soil Biol. Biochem. 23:1991;999-1002.
7. Ashbolt N.J., Inkerman P.A. Acetic acid bacterial biota of the pink sugarcane mealybug, Saccharococcus sacchari, and its environs. Appl. Environ. Microbiol. 56:1990;707-712.
8. Santo Domingo J.W. Use of 16S rDNA community fingerprints to study cricket hindgut microbial communities. J. Gen. Appl. Microbiol. 44:1998;119-127.
9. Amann R.I., Ludwig W., Schleifer K.H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:1995;143-169.
10. Amann R., Binder B.J., Olson B.J., Chisholm S.W., Devereux R., Stahl D.A. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analysing mixed microbial populations. Appl. Environ. Microbiol. 56:1990;1919-1925.
11. Amann R.I., Springer N., Ludwig W., Gortz H.D., Schleifer K.H. Identification in situ and phylogeny of uncultured bacterial endosymbionts. Nature. 351:1991;161-164.
12. Santo Domingo J.W., Kaufman M.G., Klug M.J., Tiedje J.M. Characterisation of the cricket hindgut microbiota with fluorescently labeled rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 64:1998;752-755.
13. Langendijk P.S., Schut F., Jansen G.J., Raangs G.C., Kamphuis G.R., Wilkinson M.H.F., Welling G.W. Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Appl. Environ. Microbiol. 61:1995;3069-3075.
14. Wagner M., Assmus B., Hartmann A., Hutzler P., Amann R. In situ analysis of microbial consortia in activated sludge using fluorescently labelled, rRNA-targeted oligonucleotide probes and confocal scanning laser microscopy. J. Microsc. 176:1994;181-187.
15. AL. In: Bergey's Manual of Systematic Bacteriology (Krieg, N.R. and Holt, J.G., Eds.), vol 1, pp. 268-274. The Williams and Wilkins Co., Baltimore, MD.
16. Maidak B.L., Olsen G.J., Larsen N., Overbeek R., McCaughey R., Woese C.R. The RDP (Ribosomal Database Project). Nucleic Acids Res. 25:1997;109-110.
17. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215:1991;403-410.
18. Lane, D.J. (1991) 16S/23S rRNA sequencing. In: Nucleic acid techniques in bacterial systematics (Stackebrandt, E. and Goodfellow, M., Eds.), pp. 115-147. John Wiley and Sons, Chichester.
19. Manz W., Amann R., Ludwig W., Wagner M., Schleifer K.H. Phylogenetic oligonucleotide probes for the major subclasses of Proteobacteria: Problems and solutions. Syst. Appl. Microbiol. 15:1992;593-600.
20. Manz W., Wagner M., Amann R., Schleifer K.H. In situ characterisation of the microbial consortia active in two wastewater treatment plants. Water Res. 28:1994;1715-1723.
21. Lathe R. Synthetic oligonucleotide probes deduced from amino acid sequence data. Theoretical and practical considerations. J. Mol. Biol. 183:1985;1-12.
22. Herrman B., Nystrom T., Wessel H. Detection of Neisseria gonorrhoeae from air-dried genital samples by single-tube nested PCR. J. Clin. Microbiol. 34:1996;2548-2551.
23. Juck D., Ingram J., Prevost M., Coallier J., Greer C. Nested PCR protocol for the rapid detection of Escherichia coli in potable water. Can. J. Microbiol. 42:1996;862-866.
24. Shaio M.-F., Lin P.-R., Liu J.-Y. Colorimetric one-tube nested PCR for detection of Trichomonas vaginalis in vaginal discharge. J. Clin. Microbiol. 35:1997;132-138.
25. Picken M.M., Picken R.N., Han D., Cheng Y., Strle F. Single-tube nested polymerase chain reaction assay based on flagellin gene sequences for detection of Borrelia burgdorferi sensu lato. Eur. J. Clin. Microbiol. Infect. Dis. 15:1996;489-498.
26. Amann R., Krumholz L., Stahl D.A. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic and environmental studies in microbiology. J. Bacteriol. 172:1990;762-770.
27. DeLong E., Wickham G.S., Pace N.R. Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells. Science. 243:1989;1360-1363.
28. Hahn D., Amann R.I., Zeyer J. Whole cell hybridization of Frankia strains with fluorescence- or digoxigenin-labeled, 16S rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 59:1993;2753-2757.
29. Hahn D., Amann R.I., Ludwig W., Akkermans A.D.L., Schleifer K.-H. Detection of micro-organisms in soil after in situ hybridization with rRNA-targeted, fluorescently labelled oligonucleotides. J. Gen. Microbiol. 138:1992;879-887.
30. Assmuss B., Hutzler P., Kirchhof G., Amann R., Lawrence J.R., Hartmann A. In situ location of Azospirillum brasilense in the rhizosphere of wheat with fluorescently labeled, rRNA-targeted oligonucleotide probes and scanning confocal laser microscopy. Appl. Environ. Microbiol. 61:1995;1013-1019.
31. Lloyd D., Williams A.G., Amann R., Hayes A.J., Durrant L., Ralphs J.R. Intracellular prokaryotes in rumen ciliate protozoa: Detection by confocal laser scanning microscopy after in situ hybridization with fluorescent 16S rRNA probes. Eur. J. Protistol. 32:1996;523-531.
32. Tekola P., Zhu Q., Baak J.P.A. Confocal laser microscopy and image processing for three-dimensional microscopy. Hum. Pathol. 25:1994;12-21.
33. Poulsen L.K., Ballard G., Stahl D.A. Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl. Environ. Microbiol. 59:1993;1354-1360.
34. Blakeman, J.P. and Brodle, I.D.S. (1976) Inhibition of pathogens by epiphytic bacteria on aerial plant surfaces. In: Microbiology of aerial plant surfaces (Dickinson, C.H. and Preece, T.F., Eds.), pp. 529-557. Academic Press Inc., London.
35. Woese C.R., Gutell R., Gupta R., Noller H.F. Detailed analysis of the higher-order structure of 16S-like ribosomal ribonucleic acids. Microbiol. Rev. 47:1983;621-669.