Tn917-lac mutagenesis of Streptococcus mutans to identify environmentally regulated genes

FEMS Microbiology Letters

Volumn 182, Issue 1, 2000, Pages 149-154

  Cvitkovitch, Dennis G ^a   Gutierrez, Juan A ^b   Behari, Jaideep ^c   Youngman, Philip J ^c   Wetz Jr , James E ^b   Crowley, Paula J ^b   Hillman, Jeffrey D ^b   Brady, L Jeannine ^b   Bleiweis, Arnold S ^b  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF FLORIDA   (United States)

^c University of Georgia ^*  (United States)

Author keywords

Reporter gene; Streptococcus mutans; Tn917 lac; Transposon mutagenesis

Indexed keywords

ABC TRANSPORTER; BETA GALACTOSIDASE;

AMINO ACID SEQUENCE; ARTICLE; GENE CONTROL; MUTAGENESIS; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; REPORTER GENE; SEQUENCE ANALYSIS; STREPTOCOCCUS MUTANS; TRANSPOSON;

AMINO ACID SEQUENCE; BETA-GALACTOSIDASE; CULTURE MEDIA; DNA TRANSPOSABLE ELEMENTS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; LAC OPERON; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; OPEN READING FRAMES; SEQUENCE ANALYSIS, DNA; STREPTOCOCCUS MUTANS; TEMPERATURE;

ESCHERICHIA COLI; POSIBACTERIA; STREPTOCOCCUS MUTANS;

EID: 0033987150     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(99)00579-0     Document Type: Article

References (14)

1. Cowman R.A., Baron S.S., Fitzgerald R.J. Cysteine toxicity for oral streptococci and effect of branched-chain amino acids. Infect. Immun. 39:1983;1107-1113.
2. Gutierrez J.A., Crowley P.J., Brown D.P., Hillman J.D., Youngman P., Bleiweis A.S. Insertional mutagenesis and recovery of interrupted genes of Streptococcus mutans using transposon Tn917: preliminary characterization of mutants displaying acid sensitivity and nutritional requirements. J. Bacteriol. 178:1996;4166-4175.
3. Hueck C.J., Hillen W., Saier M.H. Jr. Analysis of a cis-active sequence mediating catabolite repression in Gram-positive bacteria. Res. Microbiol. 145:1994;503-518.
4. Jagusztyn-Krynicka E.K., Hansen J.B., Crow V.L., Thomas T.D., Honeyman A.L., Curtiss R. III. Streptococcus mutans serotype c tagatose 6-phosphate pathway gene cluster. J. Bacteriol. 174:1992;6152-6158.
5. Lane M.A., Bayles K.W., Yasbin R.E. Identification and characterization of glucose-repressible promoters of Streptococcus mutans. Gene. 100:1991;225-229.
6. Maloney P.C. Bacterial transporters. Curr. Opin. Cell Biol. 6:1994;571-5821.
7. Morency H., Trahan L., Lavoie M.C. Preliminary grouping of mutacins. Can. J. Microbiol. 41:1995;826-831.
8. Peschel A., Götz F. Analysis of the Staphylococcus epidermidis genes epiF, -E, and G involved in epidermin immunity. J. Bacteriol. 178:1996;531-536.
9. Podlesek Z., Comino A., Herzog-Velikonja B., Zgur-Bertok D., Komel R., Grabnar M. Bacillus lichenoformis bacitracin-resistance ABC transporter: relationship to mammalian multidrug resistance. Mol. Microbiol. 16:1995;969-976.
10. Reizer J., Deutscher J., Sutrina S., Thompson J., Saier M. Jr. Sugar accumulation in Gram-positive bacteria: exclusion and expulsion mechanisms. Trends Biochem. Sci. 10:1985;32-35.
11. Rubens C.E., Heggen L.M., Kuypers J.M. IS861, a group B Streptococcal insertion sequence related to IS150 and IS3 of Escherichia coli. J. Bacteriol. 171:1989;5531-5535.
12. Schenk-Groninger R., Becker J., Brendel M. Cloning, sequencing, and characterization of the Lactobacillus leichmannii pyrC gene encoding dihydroorotase. Biochimie. 77:1995;265-272.
13. Simpson C.L., Russell R.R.B. Identification of a homolog of CcpA catabolite repressor protein in Streptococcus mutans. Infect. Immun. 66:1998;2085-2092.
14. Youngman, P. (1987) Plasmid vectors for recovering and exploiting Tn917 transpositions in Bacillus and other gram-positive bacteria. In: Plasmids, a Practical Approach (Hardy, K.G., Ed.), pp. 79-104. IRL Press Ltd., Oxford.