Clinically applicable multiplex PCR for four middle ear pathogens

Journal of Clinical Microbiology

Volumn 38, Issue 1, 2000, Pages 125-132

  Hendolin, Panu H ^a   Paulin, Lars ^a   Ylikoski, Jukka ^b  

^a UNIVERSITY OF HELSINKI   (Finland)

^b HELSINKI UNIVERSITY CENTRAL HOSPITAL   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

DNA GLYCOSYLTRANSFERASE; UNCLASSIFIED DRUG; URACIL N GLYCOSYLASE;

ALLOICOCCUS OTITIDIS; ARTICLE; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DNA SEQUENCE; GENE AMPLIFICATION; HAEMOPHILUS INFLUENZAE; LABORATORY AUTOMATION; MORAXELLA CATARRHALIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OTITIS MEDIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STREPTOCOCCUS PNEUMONIAE;

CHILD; DNA GLYCOSYLASES; EAR, MIDDLE; FALSE POSITIVE REACTIONS; GRAM-NEGATIVE BACTERIAL INFECTIONS; GRAM-POSITIVE BACTERIAL INFECTIONS; HAEMOPHILUS INFLUENZAE; HUMANS; MORAXELLA (BRANHAMELLA) CATARRHALIS; N-GLYCOSYL HYDROLASES; OTITIS MEDIA WITH EFFUSION; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS PNEUMONIAE; URACIL-DNA GLYCOSIDASE; URIDINE TRIPHOSPHATE;

EID: 0033986597     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Ballagi-Pordany, A., and S. Belak. 1996. The use of mimics as internal standards to avoid false negatives in diagnostic PCR. Mol. Cell. Probes 10:159-164.
2. Barany, F. 1991. Genetic disease detection and DNA amplification using cloned thermostable ligase. Proc. Natl. Acad. Sci. USA 88:189-193.
3. Baron, H., S. Fung, A. Aydin, S. Bahring, F. C. Luft, and H. Schuster. 1996. Oligonucleotide ligation assay (OLA) for the diagnosis of familial hypercholesterolemia. Nat. Biotechnol. 14:1279-1283.
4. Bosley, G. S., A. M. Whitney, J. M. Pruckler, C. W. Moss, M. Daneshvar, T. Sih, and D. F. Talkington. 1995. Characterization of ear fluid isolates of Alloiococcus otitidis from patients with recurrent otitis media. J. Clin. Microbiol. 33:2876-2880.
5. Cimino, G. D., K. C. Metchette, J. W. Tessman, J. E. Hearst, and S. T. Isaacs. 1991. Post PCR sterilization: a method to control carry-over contamination for the polymerase chain reaction. Nucleic Acids Res. 19:99-107.
6. Cone, R. W., A. C. Hobson, and M. L. W. Huang. 1992. Coamplified positive control detects inhibition of polymerase chain reactions. J. Clin. Microbiol. 30:3185-3189.
7. Edwards, U., T. Rogall, H. Blöcker, M. Emde, and E. C. Böttger. 1989. Isolation and direct complete determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res. 17:7843-7853.
8. Faden, H., and D. Dryja. 1989. Recovery of a unique organism in human middle ear fluid and its possible role in chronic otitis media. J. Clin. Microbiol. 27:2488-2491.
9. Giebink, G. S. 1989. The microbiology of otitis media. Pediatr. Infect. Dis. J. 8(Suppl 1):18-20.
10. Hendolin, P. H., A. Markkanen, J. Ylikoski, and J. J. Wahlfors. 1997. Use of multiplex PCR for simultaneous detection of four bacterial species in middle ear effusions. J. Clin. Microbiol. 35:2854-2858.
11. Hendolin, P. H., U. Kärkkäinen, A. Markkanen, T. Himi, and J. Ylikoski. 1999. High incidence of Alloiococcus otitidis in otitis media with effusion. Pediatr. Infect. Dis. J., 18:860-865.
12. Higuchi, R. 1989. Simple and rapid preparation of samples for PCR, p. 31-38. In H. A. Ehrlich (ed.), PCR technology: principles and applications for DNA amplification. Stockton Press, New York, N.Y.
13. Hotomi, M., T. Tabata, H. Kakuchi, and M. Kunimoto. 1993. Detection of Haemophilus influenzae in middle ear of otitis media with effusion by polymerase chain reaction. Int. J. Pediatr. Otorhinolaryngol. 27:119-126.
14. Jero, J., A. Virolainen, P. Salo, M. Leinonen, J. Eskola, and P. Karma. 1996. PCR assay for detecting Streptococcus pneumoniae in the middle ear of children with otitis media with effusion. Acta Otolaryngol. 116:288-292.
15. Juhn, S. K., M. M. Paparella, C. S. Kim, M. V. Goycoolea, and G. S. Giebink. 1977. Pathogenesis of otitis media. Ann. Otol. Rhinol. Laryngol. 86:481-492.
16. Kebelmann-Betzing, C., K. Seeger, S. Dragon, G. Schmitt, A. Moricke, T. A. Schild, G. Henze, and B. Beyermann. 1998. Advantages of a new Taq DNA polymerase in multiplex and time-release PCR. BioTechniques 24:154-158.
17. Krenke, C., J. S. Stephensen, and E. R. Wald. 1988. Incidence of organisms in otitis media. Ann. Otol. Rhinol. Laryngol. 87(Suppl. 133):20-21.
18. Kunakorn, M., and R. B. Markham. 1995. Clinically practical seminested PCR for Burkholderia pseudomallei quantitated by enzyme immunoassay with and without solution hybridization. J. Clin. Microbiol. 33:2131-2135.
19. Kwok, S., and R. Higuchi. 1989. Avoiding false positives. Nature 339:237-238.
20. Lazar, J. G. 1994. Advanced methods in PCR product detection. PCR Methods Appl. 4:S1-S14.
21. Longo, M. C., M. S. Berninger, and J. L. Hartley. 1990. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reaction. Gene 93:125-128.
22. Matar, G. M., N. Sidani, M. Fayad, and U. Hadi. 1998. Two-step PCR-based assay for identification of bacterial etiology of otitis media with effusion in infected Lebanese children. J. Clin. Microbiol. 36:1185-1188.
23. Pallen, M. J., and P. D. Butcher. 1991. New strategies in microbial diagnosis. J. Hosp. Infect. 18(Suppl. A):147-158.
24. Pang, J., J. Modlin, and R. Yolken. 1992. Use of modified nucleotides and utacil-DNA glycosylase (UNG) for the control of contamination in the PCR-based amplification of RNA. Mol. Cell. Probes 6:251-256.
25. Post, J. C., R. A. Preston, J. J. Aul, M. Larkins-Pettigrew, J. Rydquist-White, K. W. Anderson, R. M. Wadowsky, D. R. Reagan, E. S. Walker, L. A. Kingsley, A. E. Magit, and G. D. Ehrlich. 1995. Molecular analysis of bacterial pathogens in otitis media with effusion. JAMA 273:1598-1604.
26. Rys, P. N., and D. H. Persing. 1993. Preventing false positives: quantitative evaluation of three protocols for inactivation of polymerase chain reaction amplification products. J. Clin. Microbiol. 31:2356-2360.
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
28. Sobek, H., M. Schmidt, and K. Kaluza. 1996. Heat-labile uracil-DNA glycosylase: purification and characterization. FEBS Lett. 388:1-4.
29. Thornton, C. G., J. L. Hartley, and A. Rashtchian. 1992. Utilizing uracil DNA glycosylasc to control carryover contamination in PCR: characterization of residual UDG activity following thermal cycling. BioTechniques 13:180-184.
30. Ueyama, T., Y. Kurono, K. Shirabe, M. Takeshita, and G. Mogi. 1995. High incidence of H. influenzae in nasopharyngeal secretions and middle ear effusions as detected by PCR. J. Clin. Microbiol. 33:1835-1838.